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EvaGreen qPCR Kits

High-performance real-time PCR using BrightGreen 2X qPCR MasterMix
• Strong signals and high sensitivity due to EvaGreen fluorescent dye
• High specificity - no primer dimers, no NTC signal
• Antibody hot-start technology provides superior specificity
• Enable streamlined protocol in a simple reaction set-up
• Allow accurate quantification of a variety of gene targets
• Reduce pipetting steps to minimize the risk of contamination
• Compatible with most real-time PCR instruments

BrightGreen 2X qPCR MasterMix developed by abm provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Available with the option of ROX or fluorescein as the internal passive reference dye, BrightGreen 2X qPCR MasterMix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers.
Another advantage is the elimination of non-specific signal in the ´no template control´ (NTC) -often associated with other qPCR Mastermix formulations or miRNA qPCR amplification.

Applications
• Gene-expression analysis
• Gene knockdown validation
• Microarray validation
• High throughput applications
• Virus detection and quantification

BrightGreen Method
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When the DNA polymerase synthesizes the complementary strand to the template, the intercalating dye, BrightGreen, incorporates itself into the dsDNA and emits a strong fluorescent signal which can be detected by the thermal cycler.

High Sensitivity and High Specificity
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Compared to qPCR master mixes from other suppliers, abm’s BrightGreen 2X qPCR MasterMix is the ultimate solution for reducing non-specific amplification in qPCR, see melting curves above. The optimized buffer system works in tandem with a highly efficient HotStart DNA polymerase to minimize signal-to-noise ratio.


Please refer to the qPCR MasterMix Selection Guide (see link below) for selecting the appropriate qPCR formulation applicable to your particular instrument model.

Please note: The abm qPCR MasterMixes have been re-branded from EvaGreen to BrightGreen to reflect the renowned ISO quality standard. The catalog numbers for the qPCR MasterMixes remained the same, and there have been no changes to the proprietary, superior formulation.
Related Links

EvaGreen qRT-PCR Kits
Probe-based qPCR Kits
Probe-based qRT-PCR Kits


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Selection Guide: Find the right mix for your qPCR machine

 

 

Description Cat# Size Price    
2 x BrightGreen qPCR Mastermix with Regular ROX (4 x 1.25 ml) MasterMix-R-ABM 500 rxns (in 20 ul volumes) 267 € DETAILS   Add to Cart 
2 x BrightGreen qPCR Mastermix with Regular ROX (16 x 1.25 ml) MasterMix-R-XL-ABM 2000 rxns (in 20 ul volumes) 678 € DETAILS   Add to Cart 
5 x BrightGreen qPCR Mastermix with Regular ROX (4 x 1 ml) MasterMix-5R-ABM 1000 rxns (in 20 ul volumes) 378 € DETAILS   Add to Cart 
2 x BrightGreen qPCR Mastermix with Low ROX (4 x 1.25 ml) MasterMix-LR-ABM 500 rxns (in 20 ul volumes) 267 € DETAILS   Add to Cart 
2 x BrightGreen qPCR Mastermix with Low ROX (16 x 1.25 ml) MasterMix-LR-XL-ABM 2000 rxns (in 20 ul volumes) 678 € DETAILS   Add to Cart 
5 x BrightGreen qPCR Mastermix with Low ROX (4 x 1 ml) MasterMix-5L-ABM 1000 rxns (in 20 ul volumes) 378 € DETAILS   Add to Cart 
2 x BrightGreen qPCR Mastermix for iCycler (4 x 1.25 ml) MasterMix-iC-ABM 500 rxns (in 20 ul volumes) 267 € DETAILS   Add to Cart 
2 x BrightGreen qPCR Mastermix for iCycler (16 x 1.25 ml) MasterMix-iC-XL-ABM 2000 rxns (in 20 ul volumes) 678 € DETAILS   Add to Cart 
5 x BrightGreen qPCR Mastermix for iCycler (4 x 1 ml) MasterMix-5C-ABM 1000 rxns (in 20 ul volumes) 378 € DETAILS   Add to Cart 
2 x BrightGreen qPCR Mastermix without ROX (4 x 1.25 ml) MasterMix-S-ABM 500 rxns (in 20 ul volumes) 267 € DETAILS   Add to Cart 
2 x BrightGreen qPCR Mastermix without ROX (16 x 1.25 ml) MasterMix-S-XL-ABM 2000 rxns (in 20 ul volumes) 678 € DETAILS   Add to Cart 
5 x BrightGreen qPCR Mastermix without ROX (4 x 1 ml) MasterMix-5S-ABM 1000 rxns (in 20 ul volumes) 378 € DETAILS   Add to Cart 

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