BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516
Home -> Genomics -> PiggyBac Transposon System -> Donor Vectors for Homologous Recombination

Donor Vectors for Homologous Recombination

The PiggyBac HR Vector is designed for use as a donor vector for homologous recombination applications, such as targeted gene editing using TALEN and Cas9 technology. The basic premise of this donor vector is to be able to clone in regions homologous to upstream and downstream of the cut site as to replace (or correct) those regions with a different sequence (e.g. to remove single or multiple bases).

The PiggyBac vector contains MCS sites at the 5´ and 3´ ends of the vector that allow cloning in of homologous sequences at those ends ("HR arms"). Once these regions are cloned in, it can be transfected, along with a suitable TALEN pair, into cells for correction of the DNA sequence. Once homologous recombination has occurred in the cells, the entire construct between the 5´ and 3´ homology regions can be excised out by addition of the Super PiggyBac Transposase (see related links below), leaving only those sequences that are necessary for homologous recombination in a footprint-free manner.

Please note:
The vector is suitable for use with TALEN- and Cas9- based methods that involve homologous recombination of host genomic sequence, so the necessary TALEN pairs or Cas9 cosntructs for a given gene target must have been designed before.

Related Links

Cas9 Technology for gene editing
TALEN Technology for gene editing
Super PiggyBac Transposase Expression Vector

 

 

Description Cat# Size Price    
piggyBac HR Vector (5'MCS-EF1-GFP-Puro-TK-3'MCS) PBHR100A-1-SBI 10 ug 1296 € DETAILS   Add to Cart 

BioCat Summer Special

Summer Special

10% OFF
Norgen Sample Preparation Kits

Electrophoresis

30% OFF BioCat Universal Agarose

DNA and RNA Purification

Bioline

Use ISOLATE II Nucleic Acid Isolation Kits for the purification of high-quality DNA and RNA.

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!