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TaqMan Probe Synthesis and Purification Service
In Cooperation with Biosearch Technologies
TaqMan® Probes are dual-labeled optimized FRET probes incorporating a Black Hole Quencher dye and a fluorophore reporter molecule covalently linked to either the 3´ or 5´ end of a 20 to 30 bp oligo.
TaqMan® probes are routinely used to detect the presence and quantify the amount of specific target sequences by employing the 5´→3´ exonuclease activity of Taq polymerase and the subsequent increase in fluorescence detected during repeated amplification cycles. The TaqMan® 5´ nuclease assay offers a sensitive method to determine the presence or absence of specific target sequences and has become a staple of real-time PCR quantification techniques. In the 5´ nuclease assay, fluorescent signals are generated from the exponential range of the reaction, where component concentrations are not limiting. As a result, initial template levels can be determined with high accuracy.
BioCat offers Biosearch Technologies´ large selection of fluorophores, e.g. CAL Fluor® dyes and Quasar® dyes and BHQ dark quenchers to help you to optimize the performance of your thermocyler.
Biosearch Technologies´ fluorescent dyes such as CAL Fluor, Quasar and the BHQ Dark Quencher dyes
are fully compatible with the range of real-time PCR instruments including the Applied Bioystems´ and Stratagene real-time machines, the Corbett RotorGen 3000™, the Bio-Rad iCycler™, Cepheid SmartCycler™, Roche LightCycler™, and others.
Use the order form below to order your custom TaqMan® Probes
You can also order a primer set along with your TaqMan probe, see link below
Multiplex assays using TaqMan probes are now possible that can detect several targets simultaneously using multiple spectrally resolved fluorescent probes. BioCat is pleased to offer Biosearch Technologies´ large selection of fluorophores and dark quenchers to help you to optimize performance of your multiplex assays.
Selection Guide:
See links below for the highly stable SuperRox passive reference dye and for calibration dye standards
Use the order form below to order your custom TaqMan® Probes
Related Links
SuperRox Highly Stable Passive Reference Dye
Calibration Dyes
PDF-Downloads - Will open in new browser window
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| Custom Synthesis and Cartridge Purification of Primer (up to 30 bases) with TaqMan Probe Purchase | PPC-1-BS | 1 umol Scale (provides 225-450 nmol pure primer) | 88 € | DETAILS | Add to Cart |
| Custom Synthesis and Cartridge Purification of Primer (up to 30 bases) with TaqMan Probe Purchase | PPC-2-BS | 200 nmol Scale (provides 45-90 nmol pure primer) | 79 € | DETAILS | Add to Cart |
| Custom Synthesis and Cartridge Purification of Primer (up to 30 bases) with TaqMan Probe Purchase | PPC-5-BS | 50 nmol Scale (provides 11,25-22,5 nmol pure primer) | 77 € | DETAILS | Add to Cart |
| Custom Synthesis and Desalting of Primer (up to 30 bases) with TaqMan Probe Purchase | PP-1-BS | 1 umol Scale (provides 400-800 nmol pure primer) | 82 € | DETAILS | Add to Cart |
| Custom Synthesis and Desalting of Primer (up to 30 bases) with TaqMan Probe Purchase | PP-2-BS | 200 nmol Scale (provides 80-160 nmol pure primer) | 73 € | DETAILS | Add to Cart |
| Custom Synthesis and Desalting of Primer (up to 30 bases) with TaqMan Probe Purchase | PP-5-BS | 50 nmol Scale (provides 20-40 nmol pure primer) | 69 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-CGB1-1-BS | 1 umol Scale (provides > 60 nmol pure probe) | 702 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-CGB1-2-BS | 200 nmol Scale (provides > 25 nmol pure probe) | 460 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-CGB1-5-BS | 50 nmol Scale (provides > 10 nmol pure probe) | 340 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQplus (Black Hole Quencher) | DLO-GBP-1-BS | 1 umol (provides 60 nmol pure probe) | 1031 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQplus (Black Hole Quencher) | DLO-GBP-2-BS | 200 nmol (provides 20 nmol pure probe) | 551 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQplus (Black Hole Quencher) | DLO-GBP-5-BS | 50 nmol (provides 10 nmol pure probe) | 345 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-COB1-1-BS | 1 umol Scale (provides > 60 nmol pure probe) | 702 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-COB1-2-BS | 200 nmol Scale (provides > 25 nmol pure probe) | 460 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-COB1-5-BS | 50 nmol Scale (provides > 10 nmol pure probe) | 340 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQplus (Black Hole Quencher) | DLO-CBP-1-BS | 1 umol (provides 60 nmol pure probe) | 1031 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQplus (Black Hole Quencher) | DLO-CBP-2-BS | 200 nmol (provides 20 nmol pure probe) | 551 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQplus (Black Hole Quencher) | DLO-CBP-5-BS | 50 nmol (provides 10 nmol pure probe) | 345 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 590 Reporter (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-C590B2-1-BS | 1 umol Scale (provides > 60 nmol pure probe) | 685 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 590 Reporter (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-C590B2-2-BS | 200 nmol Scale (provides > 25 nmol pure probe) | 452 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 590 Reporter (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-C590B2-5-BS | 50 nmol Scale (provides > 10 nmol pure probe) | 340 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' Cal Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' BHQplus (Black Hole Quencher) | DLO-RBP-1-BS | 1 umol (provides 60 nmol pure probe) | 1031 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' Cal Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' BHQplus (Black Hole Quencher) | DLO-RBP-2-BS | 200 nmol (provides 20 nmol pure probe) | 551 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' Cal Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' BHQplus (Black Hole Quencher) | DLO-RBP-5-BS | 50 nmol (provides 10 nmol pure probe) | 345 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 610 Reporter (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-CAB2-1-BS | 1 umol Scale (provides > 60 nmol pure probe) | 815 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 610 Reporter (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-CAB2-2-BS | 200 nmol Scale (provides > 25 nmol pure probe) | 510 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 610 Reporter (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-CAB2-5-BS | 50 nmol Scale (provides > 10 nmol pure probe) | 364 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 635 Reporter (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-C635B2-1-BS | 1 umol Scale (provides > 40 nmol pure probe) | 927 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 635 Reporter (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-C635B2-2-BS | 200 nmol Scale (provides > 10 nmol pure probe) | 590 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' CAL Fluor Red 635 Reporter (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-C635B2-5-BS | 50 nmol Scale (provides > 3 nmol pure probe) | 411 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) / Internal T-BHQ-1 (Black Hole Quencher) / 3' C3 | DLO-F(T-B1)-1-BS | 1 umol Scale (provides > 30 nmol pure probe) | 874 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) / Internal T-BHQ-1 (Black Hole Quencher) / 3' C3 | DLO-F(T-B1)-2-BS | 200 nmol Scale (provides > 10 nmol pure probe) | 485 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-FB1-1-BS | 1 umol Scale (provides > 60 nmol pure probe) | 648 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-FB1-2-BS | 200 nmol Scale (provides > 25 nmol pure probe) | 426 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-FB1-5-BS | 100 nmol Scale (provides > 10 nmol pure probe) | 300 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQplus (Black Hole Quencher) | DLO-FBP-1-BS | 1 umol (provides 60 nmol pure probe) | 1031 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQplus (Black Hole Quencher) | DLO-FBP-2-BS | 200 nmol (provides 20 nmol pure probe) | 551 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQplus (Black Hole Quencher) | DLO-FBP-5-BS | 50 nmol (provides 10 nmol pure probe) | 345 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' TAMRA (Quencher Emission 583 nm) | DLO-FT-1-BS | 1 umol Scale (provides > 60 nmol pure probe) | 648 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' TAMRA (Quencher Emission 583 nm) | DLO-FT-2-BS | 200 nmol Scale (provides > 25 nmol pure probe) | 426 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' TAMRA (Quencher Emission 583 nm) | DLO-FT-5-BS | 100 nmol Scale (provides > 10 nmol pure probe) | 300 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-HB1-1-BS | 1 umol Scale (provides > 60 nmol pure probe) | 776 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-HB1-2-BS | 200 nmol Scale (provides > 25 nmol pure probe) | 505 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-HB1-5-BS | 50 nmol Scale (provides > 10 nmol pure probe) | 351 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' JOE Fluorophore Reporter (Emission 555 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-JB1-1-BS | 1 umol Scale (provides > 30 nmol pure probe) | 894 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' JOE Fluorophore Reporter (Emission 555 nm) 3' BHQ-1 (Black Hole Quencher) | DLO-JB1-2-BS | 200 nmol Scale (provides > 10 nmol pure probe) | 680 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' Quasar 570 Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-Q5B2-1-BS | 1 umol Scale (provides > 60 nmol pure probe) | 776 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' Quasar 570 Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-Q5B2-2-BS | 200 nmol Scale (provides > 25 nmol pure probe) | 510 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' Quasar 570 Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) | DLO-Q5B2-5-BS | 50 nmol Scale (provides > 10 nmol pure probe) | 364 € | DETAILS | Add to Cart |
| Dual Labeled Probe: 5' Quasar 670 Fluorophore Reporter (Emission 667 nm) 3' BHQplus (Black Hole Quencher) | DLO-QBP-1-BS | 1 umol (provides 60 nmol pure probe) | 1031 € | DETAILS | Add to Cart |
