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Molecular Beacon Synthesis and Purification Service

In Cooperation with Bioresearch Technologies
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Molecular Beacons (produced by Biosearch Technologies under license from the Public Health Research Insitutue, Newark, NJ) are dual-labeled FRET probes incorporating a Black Hole Quencher dye and a fluorphore reporter molecule. Molecular Beacons differ from TaqMan probes, however, due to the incorporation of a short (~5 b.p.) complementary sequence of bases at the 3´ and 5´ ends (the "stem" structure). Prior to amplification, the complementary bases of the stem hybridize forming the basic “stem-loop” structure of the Beacon. The stem raises the probe Tm and consequently molecular beacons can routinely discriminate between target sequences differing by a single nucleotide.

For detailed information see Molecular Beacon Website - Link below.

BioCat offers Biosearch Technologies´ large selection of fluorophores, e.g. CAL Fluor® dyes and Quasar® dyes and BHQ dark quenchers to help you to optimize the performance of your thermocyler.

Biosearch Technologies´ fluorescent dyes such as CAL Fluor, Quasar and the BHQ Dark Quencher dyes
are fully compatible with the range of real-time PCR instruments including the Applied Bioystems´ and Stratagene real-time machines, the Corbett RotorGen 3000™, the Bio-Rad iCycler™, Cepheid SmartCycler™, Roche LightCycler™, and others.

Use the order form below to order your custom Molecular Beacons

Multiplex assays using Molecular Beacons are now possible that can detect several targets simultaneously using multiple spectrally resolved fluorescent probes. BioCat is pleased to offer Biosearch Technologies´ large selection of fluorophores and dark quenchers to help you to optimize performance of your multiplex assays.


Selection Guide:

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See links below for calibration dye standards and SuperRox Passive Reference dye

Use the order form below to order your custom Molecular Beacons

Related Links

Molecular Beacon Website
SuperRox Passive Reference Dye
Calibration Dyes


PDF-Downloads - Will open in new browser window

Molecular Beacon Order Form

 

 

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Description Cat# Size Price    
Molecular Beacon: 3' Pulsar 650 (Emission 650 nm) 5' BHQ-2 (Black Hole Quencher) MBO-B2P6-1-BS 1 umol Scale (provides > 30 nmol pure probe) 905 € DETAILS   Add to Cart 
Molecular Beacon: 3' Pulsar 650 (Emission 650 nm) 5' BHQ-2 (Black Hole Quencher) MBO-B2P6-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 666 € DETAILS   Add to Cart 
Molecular Beacon: 3' Pulsar 650 (Emission 650 nm) 5' BHQ-2 (Black Hole Quencher) MBO-B2P6-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 624 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) MBO-CGB1-1-BS 1 umol Scale (provides > 30 nmol pure probe) 713 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) MBO-CGB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 546 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) MBO-CGB1-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 510 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-1-BS 1 umol Scale (provides > 30 nmol pure probe) 713 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 546 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 510 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) MBO-COB1-1-BS 1 umol Scale (provides > 30 nmol pure probe) 713 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) MBO-COB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 546 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) MBO-COB1-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 510 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-1-BS 1 umol Scale (provides > 30 nmol pure probe) 713 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 546 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 510 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 590 (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C590B2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 702 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 590 (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C590B2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 535 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 590 (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C590B2-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 499 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 610 (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) MBO-CAB2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 749 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 610 (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) MBO-CAB2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 578 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 610 (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) MBO-CAB2-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 535 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-1-BS 1 umol Scale (provides > 30 nmol pure probe) 749 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 578 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-5-BS 50 nmol (provides > 2 nmol pure probe) 535 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 635 (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C635B2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 749 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 635 (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C635B2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 624 € DETAILS   Add to Cart 
Molecular Beacon: 5' CAL Fluor Red 635 (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C635B2-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 535 € DETAILS   Add to Cart 
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) MBO-FB1-1-BS 1 umol Scale (provides > 40 nmol pure probe) 687 € DETAILS   Add to Cart 
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) MBO-FB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 530 € DETAILS   Add to Cart 
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) MBO-FB1-5-BS 50 nmol Scale (provides > 3 nmol pure probe) 494 € DETAILS   Add to Cart 
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-1-BS 1 umol Scale (provides > 40 nmol pure probe) 687 € DETAILS   Add to Cart 
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 530 € DETAILS   Add to Cart 
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-5-BS 50 nmol Scale (provides > 3 nmol pure probe) 494 € DETAILS   Add to Cart 
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) MBO-HB1-1-BS 1 umol Scale (provides > 30 nmol pure probe) 743 € DETAILS   Add to Cart 
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) MBO-HB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 573 € DETAILS   Add to Cart 
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) MBO-HB1-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 530 € DETAILS   Add to Cart 
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' DABCYL (Black Hole Quencher) MBO-HD-1-BS 1 umol Scale (provides > 30 nmol pure probe) 743 € DETAILS   Add to Cart 
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' DABCYL (Black Hole Quencher) MBO-HD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 573 € DETAILS   Add to Cart 
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' DABCYL (Black Hole Quencher) MBO-HD-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 530 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 570 Fluorophore Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) MBO-Q5B2-1-BS 1 umol Scale (provides > 40 nmol pure probe) 743 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 570 Fluorophore Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) MBO-Q5B2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 573 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 570 Fluorophore Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) MBO-Q5B2-5-BS 50 nmol Scale (provides > 3 nmol pure probe) 530 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 570 Fluorophore Reporter (Emission 566 nm) 3' DABCYL (Black Hole Quencher) MBO-Q5D-1-BS 1 umol Scale (provides > 40 nmol pure probe) 743 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 570 Fluorophore Reporter (Emission 566 nm) 3' DABCYL (Black Hole Quencher) MBO-Q5D-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 573 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 570 Fluorophore Reporter (Emission 566 nm) 3' DABCYL (Black Hole Quencher) MBO-Q5D-5-BS 50 nmol Scale (provides > 3 nmol pure probe) 530 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 670 (Emission 670 nm) 3' BHQ-2 (Black Hole Quencher) MBO-Q6B2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 749 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 670 (Emission 670 nm) 3' BHQ-2 (Black Hole Quencher) MBO-Q6B2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 578 € DETAILS   Add to Cart 
Molecular Beacon: 5' Qasar 670 (Emission 670 nm) 3' BHQ-2 (Black Hole Quencher) MBO-Q6B2-5-BS 50 nmol Scale (provides > 3 nmol pure probe) 535 € DETAILS   Add to Cart 
Molecular Beacon: 5' TAMRA Fluorophore Reporter (Emission 583 nm) 3' BHQ-2 (Black Hole Quencher) MBO-TB2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 713 € DETAILS   Add to Cart 
Molecular Beacon: 5' TAMRA Fluorophore Reporter (Emission 583 nm) 3' BHQ-2 (Black Hole Quencher) MBO-TB2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 546 € DETAILS   Add to Cart 
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