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SNAP-ChIP Panels of barcoded recombinant nucleosomes

Sample Normalization and Antibody Profiling for Chromatin Immunoprecipitation
• Mix of homogenous defined DNA-barcoded human recombinant nucleosomes subjected to rigorous quality control for lot-to-lot consistency
• Defined spike-in controls for ChIP and ChIP-Seq reactions
• Useful for ChIP Antibody validation and profiling
• Determine ChIP antibody specificity and target pulldown efficiency
• Monitor technical variability within experiments
• Quantitative recovery of DNA barcodes via qPCR provides useful STOP / GO
capability before advancing to NGS
• Perform consistent effector protein binding experiments

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation) uses DNA-barcoded designer nucleosomes (dNucs) bearing distinct post-translational modifications (PTMs) as nextgeneration spike-in controls for ChIP. EpiCypher’s SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the PTM of interest immunoprecipitated and processed alongside sample chromatin. Recovery of the associated DNA barcodes is deciphered by quantitative PCR (ChIPqPCR) or Next-Generation Sequencing (ChIP-seq). SNAP-ChIP provides defined standards to evaluate antibody performance and monitor technical variability in ChIP, setting it apart from other spike-in controls.

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SNAP-ChIP panels are a pool of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA.
Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA (“barcode”) at the 3’ end that can be deciphered by qPCR or next-generation sequencing. Each of the nucleosomes in the pool is wrapped by 2 DNA species allowing for an internal technical replicate.

The SNAP-ChIP K-MetStat Panel includes 1 unmodified nucleosome plus 15 modified nucleosomes with histone H3 or H4 posttranslational modifications (PTMs, created by a proprietary semi-synthetic method).

The SNAP-ChIP OncoStat Panel includes 1 unmodified nucleosome plus 7 modified nucleosomes with histone H3.3 point mutations.

Reference
SNAP-ChIP is adapted from Grzybowski AT et al (2015) Mol Cell 58: 886-889.

Related Links

SNAP-ChIP validated histone modification antibodies
Video: An introduction to SNAP ChIP

 

 

Description Cat# Size Price    
SNAP-ChIP Dual Labeled Hydrolysis Probe 18-6001-EPC 100 reactions 130 € DETAILS   Add to Cart 
SNAP-ChIP Dual Labeled Hydrolysis Probe 18-6005-EPC 500 reactions 130 € DETAILS   Add to Cart 
SNAP-ChIP K-MetStat Panel (10 ChIP Reactions) 19-1001-EPC 20 ul 422 € DETAILS   Add to Cart 
SNAP-ChIP K-MetStat Panel (100 ChIP Reactions) 19-1100-EPC 200 ul 3139 € DETAILS   Add to Cart 
SNAP-ChIP K-MetStat, Full Panel Primer Set 18-6101-EPC 100 reactions 130 € DETAILS   Add to Cart 
SNAP-ChIP K-MetStat, H3K27 MiniPanel Primer Set 18-6104-EPC 100 reactions 130 € DETAILS   Add to Cart 
SNAP-ChIP K-MetStat, H3K36 MiniPanel Primer Set 18-6105-EPC 100 reactions 130 € DETAILS   Add to Cart 
SNAP-ChIP K-MetStat, H3K4 MiniPanel Primer Set 18-6102-EPC 100 reactions 130 € DETAILS   Add to Cart 
SNAP-ChIP K-MetStat, H3K9 MiniPanel Primer Set 18-6103-EPC 100 reactions 130 € DETAILS   Add to Cart 
SNAP-ChIP K-MetStat, H4K20 MiniPanel Primer Set 18-6106-EPC 100 reactions 130 € DETAILS   Add to Cart 
SNAP-ChIP OncoStat Panel (10 ChIP Reactions) 19-2001-EPC 20 ul 422 € DETAILS   Add to Cart 
SNAP-ChIP OncoStat Panel (100 ChIP Reactions) 19-2100-EPC 200 ul 3139 € DETAILS   Add to Cart 
SNAP-ChIP OncoStat, Full Panel Primer Set 18-6201-EPC 100 reactions 130 € DETAILS   Add to Cart 

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