Home -> Genomics -> Nucleosomes -> Recombinant Nucleosome Panels

Recombinant Nucleosome Panels

• Unique mononucleosomes assembled from recombinant human histones
• Broad end-user applications
• Biotinylated 601 nucleosome positioning sequence
• With histone post-translational modifications (PTMs)
• Lack of free DNA
• Rigorous quality control

The access to epigenetic diversity in a physiological nucleosome context enables broad end-user applications, including:
Image• Nucleosome binding studies (e.g. chromatin reader binding preferences, see application data below for example using BRD4 BD-1),
• Enzyme screening assays (e.g. identification of preferred substrates),
• Antibody specificity testing (e.g. for applications that require antibody specificity in a nucleosome, rather than histone peptide context).



The biotin group on the DNA facilitates pull-down experiments. EpiCypher mononucleosomes do not contain free DNA which could alter assayed activities.

dCypher® Nucleosome Panels comprise a 96-well plate containing unique mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908, H2B-O60814, H3.1-P68431/H3.2-Q71DI3/H3.3-P84243, H4-P62805). Single and combinatorial histone post-translational modifications (PTMs) are created using a proprietary synthetic method. The nucleosomes are wrapped by 147 base pairs DNA containing a central 601-positioning sequence (Lowary and Widom 1998 J Mol Biol. (1):19 -42) with a 5’ biotin-TEG group.

All nucleosomes in the panel are subjected to EpiCypher’s rigorous quality control metrics, including: ESI-TOF mass spectrometry analysis of the modified proteins; SDS-PAGE to confirm octamer composition and purity; native PAGE to confirm nucleosome assembly and lack of free DNA; and Western blot analysis of the PTM or histone mutation (if applicable).

 

 

Description Cat# Size Price    
dCypher Nucleosome Full Panel 16-9001-EPC 96 well plate 2766 € DETAILS   Add to Cart 
dCypher K-MetStat & OncoStat Nucleosome Panel 16-9002-EPC 96 well plate 2215 € DETAILS   Add to Cart 
dCypher K-AcylStat Nucleosome Panel 16-9003-EPC 96 well plate 2215 € DETAILS   Add to Cart 
dCypher R-MetStat Nucleosome Panel 16-9004-EPC 96 well plate 1995 € DETAILS   Add to Cart 

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