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Home -> Genomics -> NGS Ultra-High-Throughput Multiplexed Sequencing -> Indexed Adapter Kits for Swift Biosciences Accel-NGS 2S Library Kits

Indexed Adapter Kits for Swift Biosciences Accel-NGS 2S Library Kits

Innovative Accel-NGS 2S Indexed Adapters for Ultra-High-Throughput Multiplexed Sequencing:
Get Better Sequencing Accuracy and Quadruple Your Throughput

Features:

• Automated sequencing of 96-768 samples per flow cell
• Expands combinations for sample pooling options
• Higher multiplexed pooling
• Eliminates misassignments (< 0.1%) and index hopping
• Inputs as low as 10 pg

Benefits:

• 4x higher throughput
• Greater flexibility in sample pooling
• Lower labor and reagent cost per sample
• More accurate de-multiplexing
• Reduced concerns of proper quantification


The Accel-NGS 2S Indexed Adapters are a portfolio of indices designed to significantly increase NGS multiplexed sequencing on the Illumina® platform.

The Accel-NGS 2S Indexed Adapters are a unique set of 96 single indices optimized to work in combination with the Illumina TruSeq® HT P5 adapter to provide up to 768 unique dual index combinations. The indices provide greater flexibility to process between 96-768 uniquely tagged samples per run and can accommodate a wider range of projects with varying sequencing depth. Additionally, the unique design eliminates misassignment and index hopping, an issue specific to Illumina’s pattern flow cells. All combined, improvements provide greater sensitivity to detect low frequency variants and greater fidelity to accelerate de-multiplexing during data analysis.

This industry-leading approach meets the growing demand from research laboratories and core laboratories for substantially higher throughput, greater flexibility with sample pooling options, and better data accuracy.


Workflow
The Accel-NGS 2S workflow is a standardized, sensitive protocol that requires as little as 10 pg of input DNA. This workflow incorporates improved purification steps to avoid index hopping.


Workflow for Accel-NGS 2S Single Indexed Adapters:

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The Accel-NGS 2S Single Indexed Adapters are fully compatible with the standard protocol and require no assay modification to incorporate the indices. This workflow contains one indexing step. During the Ligation I step, Reagent Y2 (a unique full-length Swift indexed adapter) is used to label each library. During the Ligation II step, Reagent B2 (the non-indexed P5 universal adapter) is ligated on the DNA library molecule. To sequence, a sample sheet is created by modifying the TruSeq LT template and saved as a .CSV file. Using standard pipeline analyses, each index is then associated with a particular read-pair, identifying samples for downstream analysis.





Workflow for Accel-NGS 2S Dual Indexed Adapters:

Image
The Accel-NGS 2S Dual Indexed Adapters are full-length Swift indexed P7 and TruSeq HT P5 adapters. This workflow contains two indexing steps. During the Ligation I step, Reagent Y2 (a unique full-length Swift indexed adapater) is used to label each library. During the Ligation II step, Reagent B2 (a full-length TruSeq HT P5 adapter) is used to provide unique dual indexed combinations. To sequence, a sample sheet is created by modifying the TruSeq HT template and saved as a .CSV file. When performing dual indexing, the i5 indexed adapter sequence (or its reverse compliment) is included depending on the sequence of choice. Using standard pipeline analyses, each Swift index, as well as the D50X index, are then associated with a particular read-pair, identifying samples for downstream analysis.



Product Specifications

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Required Accel-NGS 2S Library Kits are sold separately, see links below
Related Links

Accel-NGS 2S Low Input DNA Library Kit
Accel-NGS 2S PCR-Free Low Input DNA Library Kit
Accel-NGS 2S Hyb DNA Library Kit for Hybridization Capture


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Accel-NGS 2S Indexed Adapters Product Information Sheet

 

 

Description Cat# Size Price    
Accel-NGS 2S Indexed Adapter Kit - Set S1 (24 indices, 4 reactions/index) 26596-SWI 96 rxns 553 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Kit - Set S2 (24 indices, 4 reactions/index) 26696-SWI 96 rxns 553 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Kit - Set S3 (24 indices, 4 reactions/index) 26796-SWI 96 rxns 553 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Kit - Set S4 (24 indices, 4 reactions/index) 26896-SWI 96 rxns 553 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Bundled Kit. - Sets S1-S4 (96 indices, 4 reactions/index) 269384-SWI 384 rxns 2030 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Kit with MID (Molecular Identifier) - Set S1 (24 indices, 4 reactions/index) 27596-SWI 96 rxns 553 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Kit with MID (Molecular Identifier) - Set S2 (24 indices, 4 reactions/index) 27696-SWI 96 rxns 553 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Kit with MID (Molecular Identifier) - Set S3 (24 indices, 4 reactions/index) 27796-SWI 96 rxns 553 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Kit with MID (Molecular Identifier) - Set S4 (24 indices, 4 reactions/index) 27896-SWI 96 rxns 553 € DETAILS   Add to Cart 
Accel-NGS 2S Indexed Adapter Kit with MID (Molecular Identifier), Bundled Kit. - Sets S1-S4 (96 indices, 4 reactions/index) 279384-SWI 384 rxns 2030 € DETAILS   Add to Cart 
Accel-NGS 2S Dual Indexed Adapter Kit - Set S1 (192 unique combinations) 28596-SWI 192 rxns 2030 € DETAILS   Add to Cart 
Accel-NGS 2S Dual Indexed Adapter Kit - Set S2 (192 unique combinations) 28696-SWI 192 rxns 2030 € DETAILS   Add to Cart 
Accel-NGS 2S Dual Indexed Adapter Kit - Set S3 (192 unique combinations) 28796-SWI 192 rxns 2030 € DETAILS   Add to Cart 
Accel-NGS 2S Dual Indexed Adapter Kit - Set S4 (192 unique combinations) 28896-SWI 192 rxns 2030 € DETAILS   Add to Cart 
Accel-NGS 2S Dual Indexed Adapter Kit, Bundle Kit for Sets S1 - S4 (786 unique combinations) 289384-SWI 786 rxns 7935 € DETAILS   Add to Cart 

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