NGS Library Preparation from DNA enriched by ChIP or MeDIP/hmeDIP

Epigentek´s EpiNext High-Sensitivity DNA Library Preparation Kit and EpiNext DNA Library Preparation Kit were designed for fragmented dsDNA enriched from ChIP reactions, pr MeDIP/hMeDIP reactions. However they can also be used for fragmented dsDNA isolated from tissue or cell samples, or exon capture for next generation sequencing applications using an Illumina sequencer.

Epigentek´s EpiNext High-Sensitivity DNA Library Preparation Kit: The amount of DNA enriched by ChIP or MeDIP/hmeDIP is often at low or even sub-nanogram levels which causes insufficient DNA library yields. To address this issue, Epigentek developed the EpiNext™ High-Sensitivity DNA Library Preparation Kit. The input amount of DNA can be from as low as 0.2 ng up to 100 ng. For optimal preparation, the input amount should be 10 ng to 50 ng.

EpiNext DNA Library Preparation Kit: Input amount of DNA can be from 5 ng to 1 µg. For optimal preparation, the input amount should be 100 ng to 200 ng. A PCR free protocol is also possible, for which at least 500 ng input DNA is needed.


Both kits from Epigentek can be used for non-barcoded (singleplexed) and barcoded (multiplexed) DNA library preparation. An NGS Barcode Set is available separately.

 

 

Description	Cat#	Size	Price
EpiNext High-Sensitivity DNA Library Preparation Kit (Illumina)	P-1053-12-EP	12 reactions	583 €	DETAILS	Add to Cart
EpiNext High-Sensitivity DNA Library Preparation Kit (Illumina)	P-1053-24-EP	24 reactions	933 €	DETAILS	Add to Cart
EpiNext DNA Library Preparation Kit (Illumina)	P-1051-12-EP	12 reactions	583 €	DETAILS	Add to Cart
EpiNext DNA Library Preparation Kit (Illumina)	P-1051-24-EP	24 reactions	933 €	DETAILS	Add to Cart
EpiNext NGS Barcode (Index) Set-12 (Illumina)	P-1060-144-EP	144 reactions	212 €	DETAILS	Add to Cart