CUT&Tag Sequencing NGS Library Preparation

Ultra-sensitive, low-input chromatin mapping assays

CUTANA™ ChIC / CUT&Tag assays build on recent advancements in immunotethering technology to deliver a highly sensitive and cost-effective workflow for epigenomic mapping. There are numerous advantages to CUT&Tag over ChIP-seq, the standard chromatin profiling assay:

• Greater sensitivity with ultra-low inputs
• Go from samples to sequence-ready libraries in a single tube
• Streamlined, 2-day protocol
• Only 3-5 million reads required / sample
• Optimized for histone PTMs and select validated target

CUTANA™ CUT&Tag provides impressive gains compared to ChIP-seq, the leading assay for chromatin and histone PTM profiling:

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How does CUT&Tag work?

Cleavage Under Targets and Tagmentation (CUT&Tag) is a novel immunotethering assay, based on the technology used in Chromatin ImmunoCleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods.

In CUT&Tag, a fusion of protein A, protein G, and Tn5 transposase is used to catalyze simultaneous cleavage and sequencing adapter ligation at antibody-bound chromatin. The use of Tn5 in CUT&Tag is a key advance, as this step eliminates the need for chromatin fragmentation as well as expensive library preparation.

The result is a highly cost- and time-efficient NGS assay, with improved signal : noise and genomic coverage vs. ChIP-seq.


Quality data with lower input and sequencing depth requirements
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Fig.1. CUTANA™ CUT&Tag generates similar results at a fraction of the cell input and sequencing depth used in ChIP-seq. A representative 300 kb region at the LAMC3 gene is shown for CUT&Tag (orange), CUT&RUN (green) and ChIP-seq (blue) data generated using H3K4me3 and H3K27me3 antibodies (EpiCypher 13-0041 and 13-0030, respectively). Rabbit IgG Negative Control Antibody (EpiCypher 13-0042) and ChIP Input control are shown for comparison (control tracks are scaled to the track with the highest signal in each approach).


Go from cells to sequencing-ready DNA in < 2 days

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Related Links

CUTANA CUT&RUN Product Line

 

 

Description Cat# Size Price    
CUTANA pAG-Tn5 for ChIC/CUT&Tag 15-1017-EPC 50 reactions 603 € DETAILS   Add to Cart 
CUTANA pAG-Tn5 for ChIC/CUT&Tag 15-1117-EPC 250 reactions 2247 € DETAILS   Add to Cart 
CUTANA High Fidelity 2X PCR Master Mix 15-1018-EPC 50 rxns 185 € DETAILS   Add to Cart 
Anti-Rabbit Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows 13-0047-EPC 50 reactions 210 € DETAILS   Add to Cart 
Anti-Rabbit Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows 13-1047-EPC 250 reactions 360 € DETAILS   Add to Cart 
Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows 13-0048-EPC 50 reactions 210 € DETAILS   Add to Cart 
Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows 13-1048-EPC 250 reactions 360 € DETAILS   Add to Cart 

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