CUT&Tag Sequencing NGS Library PreparationUltra-sensitive, low-input chromatin mapping assays
CUTANA™ ChIC / CUT&Tag assays build on recent advancements in immunotethering technology to deliver a highly sensitive and cost-effective workflow for epigenomic mapping. There are numerous advantages to CUT&Tag over ChIP-seq, the standard chromatin profiling assay:
• Greater sensitivity with ultra-low inputs
• Go from samples to sequence-ready libraries in a single tube
• Streamlined, 2-day protocol
• Only 3-5 million reads required / sample
• Optimized for histone PTMs and select validated target
CUTANA™ CUT&Tag provides impressive gains compared to ChIP-seq, the leading assay for chromatin and histone PTM profiling:
How does CUT&Tag work?
Cleavage Under Targets and Tagmentation (CUT&Tag) is a novel immunotethering assay, based on the technology used in Chromatin ImmunoCleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods.
In CUT&Tag, a fusion of protein A, protein G, and Tn5 transposase is used to catalyze simultaneous cleavage and sequencing adapter ligation at antibody-bound chromatin. The use of Tn5 in CUT&Tag is a key advance, as this step eliminates the need for chromatin fragmentation as well as expensive library preparation.
The result is a highly cost- and time-efficient NGS assay, with improved signal : noise and genomic coverage vs. ChIP-seq.
Quality data with lower input and sequencing depth requirements
Fig.1. CUTANA™ CUT&Tag generates similar results at a fraction of the cell input and sequencing depth used in ChIP-seq. A representative 300 kb region at the LAMC3 gene is shown for CUT&Tag (orange), CUT&RUN (green) and ChIP-seq (blue) data generated using H3K4me3 and H3K27me3 antibodies (EpiCypher 13-0041 and 13-0030, respectively). Rabbit IgG Negative Control Antibody (EpiCypher 13-0042) and ChIP Input control are shown for comparison (control tracks are scaled to the track with the highest signal in each approach).
Go from cells to sequencing-ready DNA in < 2 days
|CUTANA pAG-Tn5 for ChIC/CUT&Tag||15-1017-EPC||50 reactions||603 €||DETAILS||Add to Cart|
|CUTANA pAG-Tn5 for ChIC/CUT&Tag||15-1117-EPC||250 reactions||2247 €||DETAILS||Add to Cart|
|CUTANA High Fidelity 2X PCR Master Mix||15-1018-EPC||50 rxns||185 €||DETAILS||Add to Cart|
|Anti-Rabbit Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows||13-0047-EPC||50 reactions||210 €||DETAILS||Add to Cart|
|Anti-Rabbit Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows||13-1047-EPC||250 reactions||360 €||DETAILS||Add to Cart|
|Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows||13-0048-EPC||50 reactions||210 €||DETAILS||Add to Cart|
|Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows||13-1048-EPC||250 reactions||360 €||DETAILS||Add to Cart|