CUT&RUN Sequencing NGS Library Preparation

CUTANA™ assays from EpiCypher use a proprietary immunotethering approach similar to Chromatin ImmunoCleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods. This approach can be used to map histone post-translational modifications (PTMs) and chromatin interactors with high sensitivity and resolution. The approach leverages a factor specific antibody to tether a fusion of protein A, protein G and micrococcal nuclease (pAG-MNase) to genomic binding sites in intact cells, which is then activated by the addition of calcium to cleave DNA.

Overview of the approach
Cells (or nuclei) are immobilized on lectin-coated magnetic beads, permeabilized, and incubated with an antibody to a chromatin target (e.g. histone PTM or chromatin / DNA binding protein). Next, pAG-MNase is added and activated via Ca2+. The clipped chromatin fragments diffuse out, followed by DNA purification and next-generation sequencing. CUTANA™ assays vastly outperform those using chromatin immunoprecipitation, the current gold-standard genomic mapping assay.

CUTANA CUT&RUN: Go from cells to data in < 4 Days

Image

For conversion of the Micrococcal Nuclease generated short DNA fragments into NGS DNA sequencing libraries the Accel NGS 1S DNA Library Kit fits perfectly.



References:

Skene PJ, Henikoff JG & Henikoff S (2018) Targeted in situ genome-wide profiling with high efficiency for low cell numbers. Nature Protocols 13:1006-1019 (doi:10.1038/nprot.2018.015).

Schmid M, Durussel T & LaemmliUK (2004) ChIC and ChEC; genomic mapping of chromatin proteins. Molecular Cell16:147-157 (doi:10.1016/j.molcel.2004.09.007).

Shah RN, et al. (2018) Examining the roles of H3K4 methylation states with systematically characterized antibodies. Molecular Cell72:162-177 (doi: 10.1016/j.molcel.2018.08.015).

Related Links

Accel-NGS 1S Plus DNA Library Kits

 

 

Description Cat# Size Price    
CUTANA CUT&RUN Kit 14-1048-EPC 48 sampes 963 € DETAILS   Add to Cart 
CUTANA Concanavalin A-Conjugated Paramagnetic Beads 21-1401-EPC 550 ul 238 € DETAILS   Add to Cart 
CUTANA Concanavalin A-Conjugated Paramagnetic Beads 21-1411-EPC 2,75 ml 885 € DETAILS   Add to Cart 
CUTANA pAG-MNase for ChIC/CUT&RUN Workflows 15-1016-EPC 50 rxns 379 € DETAILS   Add to Cart 
CUTANA pAG-MNase for ChIC/CUT&RUN Workflows 15-1116-EPC 250 rxns 1427 € DETAILS   Add to Cart 
CUTANA E. coli Spike-in DNA 18-1401-EPC 100 ng 162 € DETAILS   Add to Cart 
Rabbit IgG Antibody, CUTANA CUT&RUN Negative Control 13-0042-EPC 100 ug 210 € DETAILS   Add to Cart 
Histone H3K27me3 Monoclonal Antibody - SNAP-ChIP Certified, CUTANA CUT&RUN Compatible 13-0030-EPC 100 ug 571 € DETAILS   Add to Cart 
Histone H3K36me3 Monoclonal Antibody - SNAP-ChIP Certified, CUTANA CUT&RUN Compatible 13-0031-EPC 100 ug 571 € DETAILS   Add to Cart 
Histone H3K4me3 Antibody: SNAP-ChIP Certified, CUTANA CUT&RUN Compatible 13-0041-EPC 100 ug 571 € DETAILS   Add to Cart 
CUTANA DNA Purification Kit 14-0050-EPC 50 samples 280 € DETAILS   Add to Cart 
Magnetic Separation Rack, 0.2 ml Tubes 10-0008-EPC 1 rack 460 € DETAILS   Add to Cart 
Magnetic Separation Rack, 1.5 ml Tubes 10-0012-EPC 1 rack 162 € DETAILS   Add to Cart 
CUTANA CUT&RUN 8-strip 0.2mL tubes 10-0009-EPC 120 strips 154 € DETAILS   Add to Cart 

BioCat Summer Special

% Special Offers

Benefit from our current promotions

SARS-CoV-2 / COVID-19

NEW! Tools for
SARS-CoV-2
Research

Electrophoresis

30% OFF BioCat Universal Agarose

DNA and RNA Purification

Bioline

Use ISOLATE II Nucleic Acid Isolation Kits for the purification of high-quality DNA and RNA.

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!