Hybridization Capture Kits

• Enrichment of the human exome or subsets of disease-related genes
• Detection of germline inherited SNVs and Indels
• Low frequency somatic variant detection of SNVs and Indels
• Copy number variant detection
• Probes achieve deep and uniform coverage
• High quality data even across GC-rich regions such as first exons

The Hybridization Capture System consists of different probe panels which can be combined with Hyb and Wash or Hyb, Wash and Blocking Kits and a Library Amplification Primer Mix depending on the customer needs.

Choose between 3 panel variants: Human Exome, Pan-Cancer and Inherited Diseases. The panels consist of individually synthesized and quality-controlled probes designed to provide the highest level of performance and ready to hybridize to NGS libraries to enrich for targets of interest.

Compatible DNA Library Preparation Kits
Swifts´ Hybridization Capture System is compatible with the following NGS Library Preparation Kits:
Accel-NGS 2S Hyb DNA Library Kits for FFPE, cfDNA, and incorporation of molecular identifiers (MIDs) for ultra-low frequency variant detection
Swift 2S Turbo DNA Library Kits for high quality genomic DNA and FFPE
Accel-NGS 1S Plus DNA Library Kits for heavily nicked or denatured samples
• Further ligation-based library preparation kits from other vendors (not compatible with transposon-based kits)

Hybridization Capture Workflow
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Figure 1. Workflow
Libraries are pooled and combined with blocking oligos and human Cot DNA. Next, the library pool is hybridized to Swift probes, captured using streptavidin beads, and washed to remove unbound fragments. Then, the enriched library pool is amplified by PCR and is ready for multiplexed Illumina® sequencing.


Applications
• Detection of germline inherited SNVs and Indels
• Low frequency somatic variant detection of SNVs and Indels
• Copy number variant detection


Panel Specifications
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*For complete gene list please contact us


Highest On-Target Delivered by Swift Exome Hyb Panel
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Figure 2. An independent, large genome center compared the Swift Exome Hyb Panel to exome panels from other vendors. Sequencing libraries were prepared using standard shearing and ligation-based library preparation, and then pooled. The 12-plex pool was split into four 6 µg aliquots to assess each vendor’s exome panel. Target capture was performed according to each vendor’s recommended protocol. Enriched libraries were sequenced using 2 x 100 pairedended reads. On-target bases were determined with Picard (selected bases), using 35M reads per library. Results demonstrated that the Swift Exome Hyb Panel had the highest on-target mapping of the exome panels tested. The percentage of bases on target for each vendor’s panel was calculated across a 500 bp flanking region.


Swift 2S Turbo with Swift Hyb Panels Produce Better Coverage Uniformity
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Figure 3. Libraries produced with Swift 2S Turbo showed less Exome Panel probe base composition bias compared to competitor-K library prep kit in the probe dot plots. Libraries were constructed with 100 ng NA12878 DNA and sequenced on HiSeq4000 to 150x mean coverage.
Related Links

Presentation Overview Swift Hybridization Capture Kits

 

 

Description Cat# Size Price    
Swift Exome Hyb Panel (16 rxns) 83216-SWI 16 rxns 5735 € DETAILS   Add to Cart 
Swift Pan-Cancer Hyb Panel (16 rxns) 83316-SWI 16 rxns 2887 € DETAILS   Add to Cart 
Swift Inherited Diseases Hyb Panel (16 rxns) 83416-SWI 16 rxns 2887 € DETAILS   Add to Cart 
Swift Hyb and Wash Kit (16 rxns) 88016-SWI 16 rxns 378 € DETAILS   Add to Cart 
Swift Hyb, Wash, and Universal Blocker Kit (16 rxns) 89016-SWI 16 rxns 1476 € DETAILS   Add to Cart 
Swift Library Amplification Primer Mix (96 rxns) 88196-SWI 96 rxns 77 € DETAILS   Add to Cart 

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