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NGS DNA Library Preparation Kits for Targeted Sequencing (Illumina Platforms)

Amplicon Sequencing

Amplicon sequencing has emerged as a reliable and cost-effective ultra-deep targeted, next-generation sequencing (NGS) method to discover and profile known, rare, and novel variants. Amplicon sequencing provides an accurate yet economical alternative to whole genome or whole exome sequencing, allowing researchers to focus on genes of interest without the burden of high cost and cumbersome data analysis.

Applications
• Detection of genetic variations
• Screening of clinically-relevant germlines
• Investigation of low frequency somatic mutations in putative disease genes
• Suitable for low input DNA from FFPE and cfDNA
• Low frequency variant discovery and confirmation

Swift Biosciences’ advanced technology offers an array of pre-designed and customizable Accel-Amplicon™ Panels optimized for multiple sequencing platforms to enable a fast, comprehensive approach to targeted sequencing. Leveraging a combination of unique design and chemistry, the Accel-Amplicon Panels produce overlapping (tiled) amplicons in a single-tube using a rapid 2-hour workflow.


Hybridization Capture Sequencing

Targeted sequencing is a cost-effective alternative to whole genome sequencing since higher depth of coverage for specific regions can be obtained from less sequencing. Whole exome sequencing utilizing hybridization capture-based methods remains a predominant process for discovery because entire protein coding regions of genes constitute the majority of known disease causing variants. Alternatively, smaller disease specific or custom-based panels provide increased sensitivity and more efficient data analysis by focusing on targeted regions most likely to contain variants of interest. In either case, an unbiased NGS library must be generated in order to maximize the potential sensitivity in the selected hybridization capture panel.

Applications
• Enrichment of the human exome or subsets of disease-related genes
• Detection of germline inherited SNVs and Indels
• Low frequency somatic variant detection of SNVs and Indels
• Copy number variant detection
• Ultralow frequency variant detection using molecular identifiers (MIDs)
• Suitable for low input DNA from FFPE and cfDNA

Swift Biosciences offers a complete line of products from NGS Library Preparation Kits for library production to target enrichment by a Hybridization Capture System where different probe panels can be combined with Hyb and Wash or Hyb, Wash and Blocking Kits and a Library Amplification Primer Mix depending on the customer needs.

Swifts´ Hybridization Capture System is compatible with the following NGS Library Preparation Kits:

Accel-NGS 2S Hyb DNA Library Kits
Swift 2S Turbo DNA Library Kits
Accel-NGS 1S Plus DNA Library Kits

and further Ligation-based library preparation kits from other vendors (not compatible with transposon-based kits).


Related Links

Brochure Accel-Amplicon NGS Panels
Technical Note on Molecular Identifiers (MIDs)


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Swift Product Selection Guide


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