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Home -> Genomics -> Next Generation Sequencing Services -> Bisulfite (Methyl-Seq) NGS Services

Bisulfite (Methyl-Seq) NGS Services

Genome-wide, base resolution DNA methylation analysis is now made easy using Epigentek´s comprehensive Methyl-Seq bisulfite sequencing services. The next-generation sequencing platforms can deliver a great amount of useful DNA methylation information with publication-ready data parsed by their expert bioinformatics scientists. The study of methylation at single base resolution of individual cytosines in DNA is facilitated by bisulfite treatment of DNA followed by PCR amplification, cloning, and sequencing of individual amplimers. EpiGentek’s Methyl-Seq platform uses Reduced Representation Bisulfite Sequencing (RRBS) or Whole Genome Bisulfite Sequencing (WGBS) to yield reliable information on the methylation states of individual cytosines by effectively and efficiently preparing converted DNA for use in next generation sequencing techniques and offers the following advantages:

• Complete conversion (>99.9%) and maximum recovery of bisulfite-converted DNA
• Minimal selection bias, allowing for a low amount of input material high yield of the constructed DNA library, and low error rates
• Streamlined process for rapid turnaround time (6-12 weeks depending on sequencing lane availability)
• Multi-stage quality control check including antibody validation, Bioanalyzer library QC, and KAPA qPCR quantification
• Cost-reducing multiplexing of multiple libraries per sequencing lane
• Receive full deliverable package including raw sequencing files, FastQC quality control insight, Bismark read mapping, methylation calling data, differential methylation analysis, and methylation region annotation


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Three different service levels are available:

Basic Methyl-Seq Bisulfite NGS Service (RRBS): Bisulfite sequencing method utilized to detect up to 3 million unique CpG sites, covering nearly all CpG islands and gene promoters

Enhanced Methyl-Seq Bisulfite NGS Service (ERRBS): Bisulfite sequencing method utilized to detect up to 6 million unique CpG sites, covering nearly all CpG islands, gene promoters, and most genetic regulatory elements, gene bodies, and repeated DNA sequences

Complete Methyl-Seq Bisulfite NGS Service (WGBS): Bisulfite sequencing method to detect in-depth DNA methylation across the entire genome, including methylation at CpG sites and less common non-CpG site such as CNG

Pricing depends on the number of samples and your specific requirements -
please contact us for a quotation.

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Please contact us for a quotation

 

 

Description Cat# Size Price    
Basic Methyl-Seq Bisulfite NGS Services S-1BMS-EP NGS Profiling Data please inquire € DETAILS   Add to Cart 
Enhanced Methyl-Seq Bisulfite NGS Services S-1EMS-EP NGS Profiling Data please inquire € DETAILS   Add to Cart 
Complete Methyl-Seq Bisulfite NGS Services S-1CMS-EP NGS Profiling Data please inquire € DETAILS   Add to Cart 

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