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microRNA qPCR Arrays

qPCR Arrays from GeneCopoeia for high-throughput profiling of miRNA expression

• Validated primers designed with proprietary algorithm
• Detects as low as 10 pg of small RNA or 20 pg of total RNA
• Distinguish miRNAs with single nucleotide mismatches
• Simultaneously detects mRNAs at different expression levels
• High reproducibility (R2 > 0.99) for inter-array and intra-array replicates
• Largest genome-wide miRNA coverage

Pre-selected miRNA profiling arrays are devided into 3 groups of qPCR arrays:

• miRNome qPCR arrays
• Cancer miRNA qPCR arrays
• Disease and focus-group miRNA qPCR arrays


MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.

The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research.
Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer) and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction.

Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.

Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion. The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.

Important Note:
Before placing an order please take care to choose the right kit version which is compatible with your qPCR instrument (see table below)!

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Experimental Workflow

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miRNA RT-PCR Mechanism
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Performance Data

Linear Range and Sensitivity (Total RNA)

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Figure 2. Broad linear range and high sensitivity
Starting with serially diluted amounts of human colon cancer total RNA, miR-21 and miR-1260 were detected using All-in-One™ miRNA qRT-PCR Detection Kit. The resulting Ct values were plotted against the log10 of the amounts of input total RNA. The data demonstrated a broad linear dynamic range from 20pg to 2 μg of input total RNA as well as high sensitivity. This allows the detection of miRNAs at varying expression levels, including low expressers.


Inter-Array Reproducibility

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Figure 3. High inter-array reproducibility
Two miProfile PCR array replicates (plate A and B) were analyzed using human total RNA (10-tissue mix) on the Bio-Rad iQ5. The Ct values of the replicate plates were plotted against each other. R2 > 0.99 were observed for high inter-array reproducibility.R2 > 0.99 is also observed for intra-array reproducibility (data not shown).


Single Nucleotide Mismatches

(A)
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(B)
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Figure 4. Specificity of miRNA detection
miRNA miR-29a and miR-29c with one single nucleotide mismatch (B) can be distinguished. Relative detection, defined as a percentage of the perfect match (100% x 2 - ΔCt), was calculated using the Ct values of on-target and off-target assays, which were performed to detect miRNA plasmid DNA templates using All-in-One miRNA qRT-PCR Detection Kits (A).

 

 

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Description Cat# Size Price    
miProfile human miRNome miRNA qPCR arrays (ABI, plate format A) QM001-A1-GC 1 set 917 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (ABI, plate format A) QM001-A6-GC 6 sets 3550 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (ABI, plate format B) QM001-B1-GC 1 set 917 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (ABI, plate format B) QM001-B6-GC 6 sets 3550 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (Bio-Rad, plate format C) QM001-C1-GC 1 set 917 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (Bio-Rad, plate format C) QM001-C6-GC 6 sets 3550 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (Bio-Rad, plate format D) QM001-D1-GC 1 set 917 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (Bio-Rad, plate format D) QM001-D6-GC 6 sets 3550 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (Roche LightCycler 480, 96-well block) QM001-E1-GC 1 set 917 € DETAILS   Add to Cart 
miProfile human miRNome miRNA qPCR arrays (Roche LightCycler 480, 96-well block) QM001-E6-GC 6 sets 3550 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (ABI, plate format A) QM002-A1-GC 1 set 531 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (ABI, plate format A) QM002-A6-GC 6 sets 2320 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (ABI, plate format B) QM002-B1-GC 1 set 531 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (ABI, plate format B) QM002-B6-GC 6 sets 2320 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Bio-Rad, plate format C) QM002-C1-GC 1 set 531 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Bio-Rad, plate format C) QM002-C6-GC 6 sets 2320 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Bio-Rad, plate format D) QM002-D1-GC 1 set 531 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Bio-Rad, plate format D) QM002-D6-GC 6 sets 2320 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Roche LightCycler 480, 96-well block) QM002-E1-GC 1 set 531 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Roche LightCycler 480, 96-well block) QM002-E6-GC 6 sets 2320 € DETAILS   Add to Cart 
miProfile human miRNome qPCR arrays (ABI, 384-well, plate format F) QM042-F1-GC 1 set 917 € DETAILS   Add to Cart 
miProfile human miRNome qPCR arrays (ABI, 384-well, plate format F) QM042-F6-GC 6 sets 3550 € DETAILS   Add to Cart 
miProfile human miRNome qPCR arrays (Bio-Rad CFX384 384-well block) QM042-G1-GC 1 set 917 € DETAILS   Add to Cart 
miProfile human miRNome qPCR arrays (Bio-Rad CFX384 384-well block) QM042-G6-GC 6 sets 3550 € DETAILS   Add to Cart 
miProfile human miRNome qPCR arrays (Roche LightCycler 480, 384-well block) QM042-H1-GC 1 set 917 € DETAILS   Add to Cart 
miProfile human miRNome qPCR arrays (Roche LightCycler 480, 384-well block) QM042-H6-GC 6 sets 3550 € DETAILS   Add to Cart 
miProfile human miRNome qPCR arrays (ABI, 384-well, plate format F) QM043-F1-GC 1 set 531 € DETAILS   Add to Cart 
miProfile human miRNome qPCR arrays (ABI, 384-well, plate format F) QM043-F6-GC 6 sets 2320 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Bio-Rad CFX384 384-well block) QM043-G1-GC 1 set 531 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Bio-Rad CFX384 384-well block) QM043-G6-GC 6 sets 2320 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Roche LightCycler 480, 384-well block) QM043-H1-GC 1 set 531 € DETAILS   Add to Cart 
miProfile mouse miRNome miRNA qPCR arrays (Roche LightCycler 480, 384-well block) QM043-H6-GC 6 sets 2320 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (ABI, plate format A) QM003-A1-GC 1 set 92 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (ABI, plate format A) QM003-A6-GC 6 sets 260 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (ABI, plate format B) QM003-B1-GC 1 set 92 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (ABI, plate format B) QM003-B6-GC 6 sets 260 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (Bio-Rad, plate format C) QM003-C1-GC 1 set 92 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (Bio-Rad, plate format C) QM003-C6-GC 6 sets 260 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (Bio-Rad, plate format D) QM003-D1-GC 1 set 92 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (Bio-Rad, plate format D) QM003-D6-GC 6 sets 260 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (Roche LightCycler 480, 96-well block) QM003-E1-GC 1 set 92 € DETAILS   Add to Cart 
miProfile human single-nucleotide mismatch miRNA qPCR arrays (Roche LightCycler 480, 96-well block) QM003-E6-GC 6 sets 260 € DETAILS   Add to Cart 
miProfile human cancer miRNA qPCR arrays (ABI, plate format A) QM004-A1-GC 1 set 685 € DETAILS   Add to Cart 
miProfile human cancer miRNA qPCR arrays (ABI, plate format A) QM004-A6-GC 6 sets 1994 € DETAILS   Add to Cart 
miProfile human cancer miRNA qPCR arrays (ABI, plate format B) QM004-B1-GC 1 set 685 € DETAILS   Add to Cart 
miProfile human cancer miRNA qPCR arrays (ABI, plate format B) QM004-B6-GC 6 sets 1994 € DETAILS   Add to Cart 
miProfile human cancer miRNA qPCR arrays (Bio-Rad, plate format C) QM004-C1-GC 1 set 685 € DETAILS   Add to Cart 
miProfile human cancer miRNA qPCR arrays (Bio-Rad, plate format C) QM004-C6-GC 6 sets 1994 € DETAILS   Add to Cart 
miProfile human cancer miRNA qPCR arrays (Bio-Rad, plate format D) QM004-D1-GC 1 set 685 € DETAILS   Add to Cart 
miProfile human cancer miRNA qPCR arrays (Bio-Rad, plate format D) QM004-D6-GC 6 sets 1994 € DETAILS   Add to Cart 
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