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microRNA Detection by qPCR without RT Step

miRNAs are known to involve in multiple biological functions and abnormal expression is associated to human diseases such as cancers. They represent a promising new class of cancer biomarkers. A number of PCR methods have been developed for analyzing the expression level of miRNA.

Based on its proprietary technology, our partner Signosis has developed a highly sensitive and discriminative real-time PCR assay method for measuring miRNA expression. It implements oligo-ligation and SYBR green based real-time PCR.

The assay can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without cDNA conversion.

 

 

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Description Cat# Size Price    
miRNA Real-Time qPCR Kit (includes Ligation, Purification and SYBR Green qPCR Reagents, miRNA oligos not included ) CL-0004-SO 20 rxns 698 € DETAILS   Add to Cart 
Let7 Isoform Real-Time qPCR Assay Kit (includes Ligation, Purification and SYBR Green qPCR Reagents and Let7 Oligo Mix) CL-0006-SO 12 rxns 722 € DETAILS   Add to Cart 
miR-17-92 Cluster Real-Time qPCR Assay Kit (includes Ligation, Purification and SYBR Green qPCR Reagents and miR-17-92 Oligo Mix) CL-0005-SO 12 rxns 722 € DETAILS   Add to Cart 
let-7a RT PCR oligo mix PO-0001-SO 20 reactions 163 € DETAILS   Add to Cart 
let-7b RT PCR oligo mix PO-0002-SO 20 reactions 163 € DETAILS   Add to Cart 
let-7c RT PCR oligo mix PO-0003-SO 20 reactions 163 € DETAILS   Add to Cart 
let-7d RT PCR oligo mix PO-0004-SO 20 reactions 163 € DETAILS   Add to Cart 
let-7e RT PCR oligo mix PO-0005-SO 20 reactions 163 € DETAILS   Add to Cart 
let-7f RT PCR oligo mix PO-0006-SO 20 reactions 163 € DETAILS   Add to Cart 
let-7g RT PCR oligo mix PO-0007-SO 20 reactions 163 € DETAILS   Add to Cart 
let-7i RT PCR oligo mix PO-0008-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-1 RT PCR oligo mix PO-0009-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-100 RT PCR oligo mix PO-0042-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-101-1 RT PCR oligo mix PO-0043-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-103 RT PCR oligo mix PO-0044-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-106a RT PCR oligo mix PO-0045-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-106b RT PCR oligo mix PO-0046-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-107 RT PCR oligo mix PO-0047-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-10a RT PCR oligo mix PO-0012-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-122a RT PCR oligo mix PO-0048-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-124a RT PCR oligo mix PO-0049-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-125a RT PCR oligo mix PO-0050-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-125b RT PCR oligo mix PO-0051-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-126 RT PCR oligo mix PO-0052-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-127 RT PCR oligo mix PO-0053-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-128a RT PCR oligo mix PO-0054-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-128b RT PCR oligo mix PO-0055-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-131 RT PCR oligo mix PO-0056-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-132 RT PCR oligo mix PO-0057-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-133a RT PCR oligo mix PO-0058-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-133b RT PCR oligo mix PO-0059-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-134 RT PCR oligo mix PO-0060-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-135b RT PCR oligo mix PO-0061-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-136 RT PCR oligo mix PO-0062-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-137 RT PCR oligo mix PO-0063-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-140 RT PCR oligo mix PO-0064-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-141 RT PCR oligo mix PO-0065-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-142-3p RT PCR oligo mix PO-0066-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-142-5p RT PCR oligo mix PO-0067-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-143 RT PCR oligo mix PO-0068-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-144 RT PCR oligo mix PO-0069-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-145 RT PCR oligo mix PO-0070-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-146a RT PCR oligo mix PO-0071-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-146b RT PCR oligo mix PO-0072-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-148a RT PCR oligo mix PO-0073-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-149 RT PCR oligo mix PO-0074-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-150 RT PCR oligo mix PO-0075-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-151 RT PCR oligo mix PO-0076-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-152 RT PCR oligo mix PO-0078-SO 20 reactions 163 € DETAILS   Add to Cart 
miR-153 RT PCR oligo mix PO-0077-SO 20 reactions 163 € DETAILS   Add to Cart 
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