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Home -> Genomics -> Methylated Histone Detection -> Methylated Histone Quantification Assays

Methylated Histone Quantification Assays

Methylated Histones play a critical role in the epigenetic regulation of gene transcription. Especially methylation of histone 3 at lysines 4, 9, 27, 36, and 79 (H3-K4, H3-K9, H3-K27, H3-K36, H3-K79) and methylation of histone 4 at lysine 20 (H4-K20) are involved in pathological processes such as cancer progress.

BioCat is pleased to offer Epigentek´s kits for the measurement of H3 and H4 methylation in situ and in vitro.

Two assay variants are available:

H3 Methylation Assay Kits

The EpiQuik™ Global Histone H3 Methylation Assay Kits are suitable for measuring site specific histone H3 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

Features and Benefits:

• Quick and efficient (finished within 5 hours).
• Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
• High sensitivity and wide detection range. control standard included
• Strip microwell format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

Image

The histone proteins are stably spotted on the strip wells. Methylated H3 histones are recognized by high-affinity antibody. The ratio or amount of methylated H3 is quantified through an HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.


H3 and H4 Methylation Quantification Kits

The EpiQuik™ Global Histone H3 and H4 Quantification Kits (Colorimetric or Fluorometric) allow to measure site specific global mono-, di-, tri-, or pan- histone H3/H4 methylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

Features and Benefits:

• Quick and efficient (finished within 2.5 hours).
• Innovative colorimetric or fluorometric assay with no need for radioactivity, electrophoresis, and chromatography.
• High sensitivity and wide detection range. control standard included
• Strip microwell format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

Image

Specific methylated histone H3/H4 is captured to the strip wells coated with corresponding antibody. The captured methylated histone H3/H4 can then be detected with a labeled detection antibody followed by color or fluorescence development reagent. The ratio of the specific methylated H3/H4 is proportional to the intensity of absorbance/fluorescence. The absolute methylated H3/H4 amount can be quantitated by comparing to the standard control.

A kit for quantifying total histone 3 amounts is also available.


Kits for Chromatin Immunoprecipitation (ChIP) for identifying DNA interaction partners of methylated histones are also available, see link below.
Related Links

Histone Extraction Kit
Histone Peptide Substrates
ChIP Kits for H3-K4 and H3-K9 DNA Target Screening

 

 

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Description Cat# Size Price    
EpiQuik In Situ Histone H3-K4 Methylation Assay Kit (Colorimetric) P-3015-096-EP 96 assays 513 € DETAILS   Add to Cart 
EpiQuik In Situ Histone H3-K4 Methylation Assay Kit (Colorimetric) P-3015-192-EP 192 assays 814 € DETAILS   Add to Cart 
EpiQuik In Situ Histone H3-K9 Methylation Assay Kit (Colorimetric) P-3016-096-EP 96 assays 513 € DETAILS   Add to Cart 
EpiQuik In Situ Histone H3-K9 Methylation Assay Kit (Colorimetric) P-3016-192-EP 192 assays 814 € DETAILS   Add to Cart 
EpiQuik In Situ Histone H3-K27 Tri-Methylation Assay Kit (Colorimetric) P-3014T-096-EP 96 assays 513 € DETAILS   Add to Cart 
EpiQuik In Situ Histone H3-K27 Tri-Methylation Assay Kit (Colorimetric) P-3014T-192-EP 192 assays 814 € DETAILS   Add to Cart 
EpiQuik Global Histone H3-K4 Methylation Assay Kit (Colorimetric) P-3017-48-EP 48 assays 496 € DETAILS   Add to Cart 
EpiQuik Global Histone H3-K4 Methylation Assay Kit (Colorimetric) P-3017-96-EP 96 assays 690 € DETAILS   Add to Cart 
EpiQuik Global Histone H3-K9 Methylation Assay Kit (Colorimetric) P-3018-48-EP 48 assays 496 € DETAILS   Add to Cart 
EpiQuik Global Histone H3-K9 Methylation Assay Kit (Colorimetric) P-3018-96-EP 96 assays 690 € DETAILS   Add to Cart 
EpiQuik Global Histone H3-K27 Methylation Assay Kit (Colorimetric) P-3020-48-EP 48 assays 496 € DETAILS   Add to Cart 
EpiQuik Global Histone H3-K27 Methylation Assay Kit (Colorimetric) P-3020-96-EP 96 assays 690 € DETAILS   Add to Cart 
EpiQuik Global Histone H3-K27 Tri-Methylation Assay Kit (Colorimetric) P-3020T-48-EP 48 assays 496 € DETAILS   Add to Cart 
EpiQuik Global Histone H3-K27 Tri-Methylation Assay Kit (Colorimetric) P-3020T-96-EP 96 assays 690 € DETAILS   Add to Cart 
EpiQuik Total Histone H3 Quantification Kit (Colorimetric) P-3062-48-EP 48 assays 373 € DETAILS   Add to Cart 
EpiQuik Total Histone H3 Quantification Kit (Colorimetric) P-3062-96-EP 96 assays 598 € DETAILS   Add to Cart 
EpiQuik Total Histone H3 Quantification Kit (Fluorometric) P-3063-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Total Histone H3 Quantification Kit (Fluorometric) P-3063-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Total Histone H4 Quantification Kit (Colorimetric) P-3072-48-EP 48 assays 366 € DETAILS   Add to Cart 
EpiQuik Total Histone H4 Quantification Kit (Colorimetric) P-3072-96-EP 96 assays 599 € DETAILS   Add to Cart 
EpiQuik Total Histone H4 Quantification Kit (Fluorometric) P-3073-48-EP 48 assays 366 € DETAILS   Add to Cart 
EpiQuik Total Histone H4 Quantification Kit (Fluorometric) P-3073-96-EP 96 assays 599 € DETAILS   Add to Cart 
EpiQuik Global Mono-Methyl Histone H3-K4 Quantification Kit (Colorimetric) P-3024-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Mono-Methyl Histone H3-K4 Quantification Kit (Colorimetric) P-3024-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Mono-Methyl Histone H3-K4 Quantification Kit (Fluorometric) P-3025-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Mono-Methyl Histone H3-K4 Quantification Kit (Fluorometric) P-3025-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Di-Methyl Histone H3-K4 Quantification Kit (Colorimetric) P-3022-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Di-Methyl Histone H3-K4 Quantification Kit (Colorimetric) P-3022-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Di-Methyl Histone H3-K4 Quantification Kit (Fluorometric) P-3023-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Di-Methyl Histone H3-K4 Quantification Kit (Fluorometric) P-3023-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Tri-Methyl Histone H3-K4 Quantification Kit (Colorimetric) P-3026-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Tri-Methyl Histone H3-K4 Quantification Kit (Colorimetric) P-3026-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Tri-Methyl Histone H3-K4 Quantification Kit (Fluorometric) P-3027-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Tri-Methyl Histone H3-K4 Quantification Kit (Fluorometric) P-3027-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Pan-Methyl Histone H3-K4 Quantification Kit (Colorimetric) P-3028-96-EP 96 assays 617 € DETAILS   Add to Cart 
EpiQuik Global Pan-Methyl Histone H3-K4 Quantification Kit (Fluorometric) P-3029-96-EP 96 assays 617 € DETAILS   Add to Cart 
EpiQuik Global Mono-Methyl Histone H3-K9 Quantification Kit (Colorimetric) P-3030-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Mono-Methyl Histone H3-K9 Quantification Kit (Colorimetric) P-3030-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Mono-Methyl Histone H3-K9 Quantification Kit (Fluorometric) P-3031-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Mono-Methyl Histone H3-K9 Quantification Kit (Fluorometric) P-3031-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Di-Methyl Histone H3-K9 Quantification Kit (Colorimetric) P-3032-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Di-Methyl Histone H3-K9 Quantification Kit (Colorimetric) P-3032-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Di-Methyl Histone H3-K9 Quantification Kit (Fluorometric) P-3033-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Di-Methyl Histone H3-K9 Quantification Kit (Fluorometric) P-3033-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Tri-Methyl Histone H3-K9 Quantification Kit (Colorimetric) P-3034-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Tri-Methyl Histone H3-K9 Quantification Kit (Colorimetric) P-3034-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Tri-Methyl Histone H3-K9 Quantification Kit (Fluorometric) P-3035-48-EP 48 assays 376 € DETAILS   Add to Cart 
EpiQuik Global Tri-Methyl Histone H3-K9 Quantification Kit (Fluorometric) P-3035-96-EP 96 assays 618 € DETAILS   Add to Cart 
EpiQuik Global Pan-Methyl Histone H3-K9 Quantification Kit (Colorimetric) P-3036-96-EP 96 assays 617 € DETAILS   Add to Cart 
EpiQuik Global Pan-Methyl Histone H3-K9 Quantification Kit (Fluorometric) P-3037-96-EP 96 assays 617 € DETAILS   Add to Cart 
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