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Methylated Histone Detection

Histones are primary protein components of eukaryotic chromatin and play a role in gene regulation. H3 and H4 histones have tails protruding from the nucleosome that can be modified post-translationally to alter the histone´s interactions with DNA and nuclear proteins, leading to epigenetic changes for regulating many normal and disease-related processes.

Histone Methylation
Histone methylation causes transcription repression or activation, depending on the target sites. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. Measurement of histone methyltrasferase activity and quantification of histone methylation patterns have become pivotal in studying epigenetic regulation of genes, as well as inhibitor discovery.

Histone Methylation Quantification Assays
Histone methylation is the modification of certain amino acids in a histone protein by the addition of methyl groups. Methylation and demethylation of histones turn genes on and off in DNA. Quantifying histone methylation is particularly useful for studying gene expression patterns.

Histone Methyltransferase Assays
Histone methyltransferases (HMTs) are enzymes that catalyze the transfer of methyl groups to histone proteins, and control or regulate DNA methylation through chromatin-dependent transcription repression or activation. Histone methylation serves in both epigenetic gene activation and silencing, thereby making it important to measure the activity or inhibition of HMTs.

Histone Demethylase Assays
Histone demethylation is the removal of methyl groups in modified histone proteins via histone demethylases. The discovery of histone demethylases demonstrates that histone methylation is not a permanent modification, but rather a more dynamic process. Specific demethylases are found to be involved in some pathological processes such as cancer progression, making it necessary to better understand the activity or inhibition of histone demethylases.

Related Links

Histone Demethylase Assays
HMT Assays: Histone Methyl Transferase Assays
Acetylated Histone Quantification Assays
HDAC Assays: Histone Deacetylase Assays
HAT Assays: Histone Acetyl Transferase Assays
Phosphorylated Histone Quantification Assays
Protein Sumoylation Assays

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