Recombinant CAS9 Nucleases and Nickases
Transfection-ready Cas9 Proteins (Wildtype & Variants)With transfection-ready Cas9 protein you can get more efficient genome editing with fewer off-target events. Simply pre-incubate Cas9 protein with gRNA and then transfect or electroporate into your cells or model system.
• Highly efficient Cas9 Protein
• Nuclear Localization Signal (NLS) aids delivery to the nucleus
• Simplify delivery to cells and embryos
• Reduce off-target events
• Reduce the potential for immune response
• Perform multiplex, high-throughput studies
• Disease model generation of organisms and cell lines
Taking the power of CRISPR/Cas9 technology to the next level, transfectable/electroporatable Cas9 protein delivers more efficient genome editing while reducing off-target events. By removing plasmid delivery of Cas9 from the process, both off-target events from random plasmid integration and the potential for an immune response from bacterial plasmid sequences are avoided. In addition, the more transient nature of Cas9 protein compared to plasmid/mRNA delivery further reduces off-target activity without decreasing on-target efficiency.
The end result is better - safer Cas9 activity for applications where off-target events need to be minimized, such as:
• Genome engineering in embryos
• Disease model generation of organisms and cell lines
• In vitro transfection of cells
• In vitro cleavage assays for functional gRNA screens
Related Links
T7 gRNA Cloning and Production Kit
Custom gRNA Synthesis
RNP Delivery Reagent
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| CRISPRcraft S.p. Cas9 Nuclease, 120 ug at 10 mg/ml (62 uM) | 70020-1-LU | 120 ug | 301 € | DETAILS | Add to Cart |
| CRISPRcraft S.p. Cas9 Nuclease, 400 ug at 10 mg/ml (62 uM) | 70020-2-LU | 400 ug | 607 € | DETAILS | Add to Cart |
| CRISPRcraft S.p. Cas9 Nuclease Control Kit | 70030-1-LU | 96 rxn | 212 € | DETAILS | Add to Cart |
| Recombinant Cas9 protein with NLS | GE001-GC | 25 ug | 148 € | DETAILS | Add to Cart |
| Recombinant Cas9 protein with NLS | GE002-GC | 100 ug | 230 € | DETAILS | Add to Cart |
| Cas9 Nuclease Protein | K008-ABM | 50 pmol | 67 € | DETAILS | Add to Cart |
| Cas9 Nuclease Protein | K108-ABM | 250 pmol | 124 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nuclease | M1094-50-BV | 50 pmol | 279 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nuclease | M1094-250-BV | 250 pmol | 451 € | DETAILS | Add to Cart |
| Recombinant Cas9 Protein (derived from Streptococcus pyogenes) with NLS (Nuclear localization sequence) | CAS400A-1-SBI | 50 ug | 480 € | DETAILS | Add to Cart |
| Kit: Recombinant Cas9 Protein (derived from Streptococcus pyogenes) with NLS (50 ug) plus T7 gRNA Cloning and Production Kit (10 rxns) | CAS400A-KIT-SBI | 1 kit | 1111 € | DETAILS | Add to Cart |
| Recombinant Cas9 Protein (derived from Streptococcus pyogenes) with NLS (from SV40 T antigen) on N- and C-terminus and HIS-tag on N-terminus | TP790148-OR | 500 pmol | 105 € | DETAILS | Add to Cart |
| Recombinant Cas9 protein with NLS (Nuclear localization sequence) and C-terminal 6x His-tag | GE003-GC | 25 ug | 148 € | DETAILS | Add to Cart |
| Recombinant Cas9 protein with NLS (Nuclear localization sequence) and C-terminal 6x His-tag | GE004-GC | 100 ug | 230 € | DETAILS | Add to Cart |
| Cas9 Nuclease Protein with NLS (Nuclear Localization Signal) | K030-ABM | 50 pmol | 67 € | DETAILS | Add to Cart |
| Cas9 Nuclease Protein with NLS (Nuclear Localization Signal) | K130-ABM | 250 pmol | 124 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nuclease with NLS (Nuclear localization sequence) | M1095-50-BV | 50 pmol | 279 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nuclease with NLS (Nuclear localization sequence) | M1095-250-BV | 250 pmol | 451 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 GFP with NLS (Nuclear Localization Signal) | M1284-50-BV | 50 pmol | 235 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 GFP with NLS (Nuclear Localization Signal) | M1284-250-BV | 250 pmol | 474 € | DETAILS | Add to Cart |
| Gene Snipper SaCas9 with NLS (Nuclear Localization Signal) | M1281-50-BV | 50 pmol | 235 € | DETAILS | Add to Cart |
| Gene Snipper SaCas9 with NLS (Nuclear Localization Signal) | M1281-250-BV | 250 pmol | 474 € | DETAILS | Add to Cart |
| Gene Snipper SaCas9 Protein (from Staphylococcus aureus) | M1280-50-BV | 50 pmol | 235 € | DETAILS | Add to Cart |
| Gene Snipper SaCas9 Protein (from Staphylococcus aureus) | M1280-250-BV | 250 pmol | 474 € | DETAILS | Add to Cart |
| Cas9 Nickase D10A Protein with NLS (Nuclear Localization Signal) | K034-ABM | 50 pmol | 120 € | DETAILS | Add to Cart |
| Cas9 Nickase D10A Protein with NLS (Nuclear Localization Signal) | K134-ABM | 250 pmol | 205 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase D10A with NLS (Nuclear localization sequence) | M1097-50-BV | 50 pmol | 279 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase D10A with NLS (Nuclear localization sequence) | M1097-250-BV | 250 pmol | 451 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase GFP with NLS (Nuclear Localization Signal) | M1285-50-BV | 50 pmol | 235 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase GFP with NLS (Nuclear Localization Signal) | M1285-250-BV | 250 pmol | 474 € | DETAILS | Add to Cart |
| Cas9 Nickase D10A Protein | K032-ABM | 50 pmol | 120 € | DETAILS | Add to Cart |
| Cas9 Nickase D10A Protein | K132-ABM | 250 pmol | 205 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase D10A | M1096-50-BV | 50 pmol | 279 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase D10A | M1096-250-BV | 250 pmol | 451 € | DETAILS | Add to Cart |
| Cas9 Nickase H840A Protein with NLS (Nuclear Localization Signal) | K038-ABM | 50 pmol | 120 € | DETAILS | Add to Cart |
| Cas9 Nickase H840A Protein with NLS (Nuclear Localization Signal) | K138-ABM | 250 pmol | 205 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase H840A with NLS (Nuclear localization sequence) | M1099-250-BV | 250 pmol | 451 € | DETAILS | Add to Cart |
| Cas9 Nickase H840A Protein | K036-ABM | 50 pmol | 120 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase H840A | M1098-50-BV | 50 pmol | 279 € | DETAILS | Add to Cart |
| Cas9 Nickase H840A Protein | K136-ABM | 250 pmol | 205 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase H840A | M1098-250-BV | 250 pmol | 451 € | DETAILS | Add to Cart |
| Cas9 Null Mutant Protein with NLS (Nuclear Localization Signal) | K042-ABM | 50 pmol | 120 € | DETAILS | Add to Cart |
| Cas9 Null Mutant Protein with NLS (Nuclear Localization Signal) | K142-ABM | 250 pmol | 205 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Null Protein with NLS (Nuclear localization sequence) | M1103-50-BV | 50 pmol | 279 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Null Protein with NLS (Nuclear localization sequence) | M1103-250-BV | 250 pmol | 451 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 GFP Null Protein with NLS (Nuclear Localization Signal) | M1286-50-BV | 50 pmol | 235 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 Nickase H840A with NLS (Nuclear localization sequence) | M1099-50-BV | 50 pmol | 279 € | DETAILS | Add to Cart |
| Gene Snipper Cas9 GFP Null Protein with NLS (Nuclear Localization Signal) | M1286-250-BV | 250 pmol | 474 € | DETAILS | Add to Cart |
| Gene Snipper SaCas9 Null Protein with NLS (Nuclear Localization Signal) | M1283-50-BV | 50 pmol | 235 € | DETAILS | Add to Cart |
| Gene Snipper SaCas9 Null Protein with NLS (Nuclear Localization Signal) | M1283-250-BV | 250 pmol | 474 € | DETAILS | Add to Cart |
