Recombinant CAS9 Nucleases and Nickases
Transfection-ready Cas9 Proteins (Wildtype & Variants)With transfection-ready Cas9 protein you can get more efficient genome editing with fewer off-target events. Simply pre-incubate Cas9 protein with gRNA and then transfect or electroporate into your cells or model system.
• Highly efficient Cas9 Protein
• Nuclear Localization Signal (NLS) aids delivery to the nucleus
• Simplify delivery to cells and embryos
• Reduce off-target events
• Reduce the potential for immune response
• Perform multiplex, high-throughput studies
• Disease model generation of organisms and cell lines
Taking the power of CRISPR/Cas9 technology to the next level, transfectable/electroporatable Cas9 protein delivers more efficient genome editing while reducing off-target events. By removing plasmid delivery of Cas9 from the process, both off-target events from random plasmid integration and the potential for an immune response from bacterial plasmid sequences are avoided. In addition, the more transient nature of Cas9 protein compared to plasmid/mRNA delivery further reduces off-target activity without decreasing on-target efficiency.
The end result is better - safer Cas9 activity for applications where off-target events need to be minimized, such as:
• Genome engineering in embryos
• Disease model generation of organisms and cell lines
• In vitro transfection of cells
• In vitro cleavage assays for functional gRNA screens
Related Links
T7 gRNA Cloning and Production Kit
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| Recombinant Cas9 protein with NLS | GE001-GC | 25 ug | 153 € | DETAILS | Add to Cart |
| Recombinant Cas9 protein with NLS | GE002-GC | 100 ug | 237 € | DETAILS | Add to Cart |
| Cas9 Nuclease Protein | K008-ABM | 50 pmol | 50 € | DETAILS | Add to Cart |
| Cas9 Nuclease Protein | K108-ABM | 250 pmol | 93 € | DETAILS | Add to Cart |
| Recombinant Cas9 protein with NLS (Nuclear localization sequence) and C-terminal 6x His-tag | GE003-GC | 25 ug | 153 € | DETAILS | Add to Cart |
| Recombinant Cas9 protein with NLS (Nuclear localization sequence) and C-terminal 6x His-tag | GE004-GC | 100 ug | 237 € | DETAILS | Add to Cart |
| Cas9 Nuclease Protein with NLS (Nuclear Localization Signal) | K030-ABM | 50 pmol | 50 € | DETAILS | Add to Cart |
| Cas9 Nuclease Protein with NLS (Nuclear Localization Signal) | K130-ABM | 250 pmol | 93 € | DETAILS | Add to Cart |
| Gene Snipper SaCas9 Protein (from Staphylococcus aureus) | M1280-50-BV | 50 pmol | please inquire € | DETAILS | Add to Cart |
| Gene Snipper SaCas9 Protein (from Staphylococcus aureus) | M1280-250-BV | 250 pmol | please inquire € | DETAILS | Add to Cart |
| Cas9 Nickase D10A Protein with NLS (Nuclear Localization Signal) | K034-ABM | 50 pmol | 67 € | DETAILS | Add to Cart |
| Cas9 Nickase D10A Protein with NLS (Nuclear Localization Signal) | K134-ABM | 250 pmol | 136 € | DETAILS | Add to Cart |
| Cas9 Nickase D10A Protein | K032-ABM | 50 pmol | 67 € | DETAILS | Add to Cart |
| Cas9 Nickase D10A Protein | K132-ABM | 250 pmol | 136 € | DETAILS | Add to Cart |
| Cas9 Nickase H840A Protein with NLS (Nuclear Localization Signal) | K038-ABM | 50 pmol | 67 € | DETAILS | Add to Cart |
| Cas9 Nickase H840A Protein with NLS (Nuclear Localization Signal) | K138-ABM | 250 pmol | 136 € | DETAILS | Add to Cart |
| Cas9 Nickase H840A Protein | K036-ABM | 50 pmol | 67 € | DETAILS | Add to Cart |
| Cas9 Nickase H840A Protein | K136-ABM | 250 pmol | 136 € | DETAILS | Add to Cart |
| Cas9 Null Mutant Protein with NLS (Nuclear Localization Signal) | K042-ABM | 50 pmol | 67 € | DETAILS | Add to Cart |
| Cas9 Null Mutant Protein with NLS (Nuclear Localization Signal) | K142-ABM | 250 pmol | 136 € | DETAILS | Add to Cart |
| Cas9 Null Mutant Protein | K040-ABM | 50 pmol | 67 € | DETAILS | Add to Cart |
| Cas9 Null Mutant Protein | K140-ABM | 250 pmol | 136 € | DETAILS | Add to Cart |
