BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516
Home -> Genomics -> Genome Engineering -> Recombinant CAS9 Nucleases and Nickases

Recombinant CAS9 Nucleases and Nickases

Transfection-ready Cas9 Proteins (Wildtype & Variants)

With transfection-ready Cas9 protein you can get more efficient genome editing with fewer off-target events. Simply pre-incubate Cas9 protein with gRNA and then transfect or electroporate into your cells or model system.

• Highly efficient Cas9 Protein
• Nuclear Localization Signal (NLS) aids delivery to the nucleus
• Simplify delivery to cells and embryos
• Reduce off-target events
• Reduce the potential for immune response
• Perform multiplex, high-throughput studies
• Disease model generation of organisms and cell lines

Taking the power of CRISPR/Cas9 technology to the next level, transfectable/electroporatable Cas9 protein delivers more efficient genome editing while reducing off-target events. By removing plasmid delivery of Cas9 from the process, both off-target events from random plasmid integration and the potential for an immune response from bacterial plasmid sequences are avoided. In addition, the more transient nature of Cas9 protein compared to plasmid/mRNA delivery further reduces off-target activity without decreasing on-target efficiency.

The end result is better - safer Cas9 activity for applications where off-target events need to be minimized, such as:

• Genome engineering in embryos
• Disease model generation of organisms and cell lines
• In vitro transfection of cells
• In vitro cleavage assays for functional gRNA screens

Related Links

T7 gRNA Cloning and Production Kit
Custom gRNA Synthesis

 

Description Cat# Size Price    
Recombinant Cas9 Protein (derived from Streptococcus pyogenes) with NLS CAS400A-1-SBI 50 ug 468 € DETAILS   Add to Cart 
Kit: Recombinant Cas9 Protein (derived from Streptococcus pyogenes) with NLS (50 ug) plus T7 gRNA Cloning and Production Kit (10 rxns) CAS400A-KIT-SBI 1 kit 1084 € DETAILS   Add to Cart 
Recombinant Cas9 Protein (derived from Streptococcus pyogenes) with NLS (from SV40 T antigen) on N- and C-terminus and HIS-tag on N-terminus TP790148-OR 500 pmol 105 € DETAILS   Add to Cart 
Cas9 Nuclease Protein with NLS (Nuclear Localization Signal) K030-ABM 50 pmol 130 € DETAILS   Add to Cart 
Cas9 Nuclease Protein with NLS (Nuclear Localization Signal) K130-ABM 250 pmol 310 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nuclease with NLS (Nuclear localization sequence) M1095-50-BV 50 pmol 265 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nuclease with NLS (Nuclear localization sequence) M1095-250-BV 250 pmol 450 € DETAILS   Add to Cart 
Gene Snipper Cas9 GFP with NLS (Nuclear Localization Signal) M1284-50-BV 50 pmol 275 € DETAILS   Add to Cart 
Gene Snipper Cas9 GFP with NLS (Nuclear Localization Signal) M1284-250-BV 250 pmol 485 € DETAILS   Add to Cart 
Cas9 Nuclease Protein K008-ABM 50 pmol 130 € DETAILS   Add to Cart 
Cas9 Nuclease Protein K108-ABM 250 pmol 310 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nuclease M1094-50-BV 50 pmol 265 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nuclease M1094-250-BV 250 pmol 450 € DETAILS   Add to Cart 
Gene Snipper SaCas9 with NLS (Nuclear Localization Signal) M1281-50-BV 50 pmol 275 € DETAILS   Add to Cart 
Gene Snipper SaCas9 with NLS (Nuclear Localization Signal) M1281-250-BV 250 pmol 485 € DETAILS   Add to Cart 
Gene Snipper SaCas9 Protein (from Staphylococcus aureus) M1280-50-BV 50 pmol 275 € DETAILS   Add to Cart 
Gene Snipper SaCas9 Protein (from Staphylococcus aureus) M1280-250-BV 250 pmol 485 € DETAILS   Add to Cart 
Cas9 Nickase D10A Protein with NLS (Nuclear Localization Signal) K034-ABM 50 pmol 130 € DETAILS   Add to Cart 
Cas9 Nickase D10A Protein with NLS (Nuclear Localization Signal) K134-ABM 250 pmol 310 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase D10A with NLS (Nuclear localization sequence) M1097-50-BV 50 pmol 265 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase D10A with NLS (Nuclear localization sequence) M1097-250-BV 250 pmol 450 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase GFP with NLS (Nuclear Localization Signal) M1285-50-BV 50 pmol 275 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase GFP with NLS (Nuclear Localization Signal) M1285-250-BV 250 pmol 485 € DETAILS   Add to Cart 
Cas9 Nickase D10A Protein K032-ABM 50 pmol 130 € DETAILS   Add to Cart 
Cas9 Nickase D10A Protein K132-ABM 250 pmol 310 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase D10A M1096-50-BV 50 pmol 265 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase D10A M1096-250-BV 250 pmol 450 € DETAILS   Add to Cart 
Cas9 Nickase H840A Protein with NLS (Nuclear Localization Signal) K038-ABM 50 pmol 130 € DETAILS   Add to Cart 
Cas9 Nickase H840A Protein with NLS (Nuclear Localization Signal) K138-ABM 250 pmol 310 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase H840A with NLS (Nuclear localization sequence) M1099-50-BV 50 pmol 265 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase H840A with NLS (Nuclear localization sequence) M1099-250-BV 250 pmol 450 € DETAILS   Add to Cart 
Cas9 Nickase H840A Protein K036-ABM 50 pmol 130 € DETAILS   Add to Cart 
Cas9 Nickase H840A Protein K136-ABM 250 pmol 310 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase H840A M1098-50-BV 50 pmol 265 € DETAILS   Add to Cart 
Gene Snipper Cas9 Nickase H840A M1098-250-BV 250 pmol 450 € DETAILS   Add to Cart 
Cas9 Null Mutant Protein with NLS (Nuclear Localization Signal) K042-ABM 50 pmol 130 € DETAILS   Add to Cart 
Cas9 Null Mutant Protein with NLS (Nuclear Localization Signal) K142-ABM 250 pmol 310 € DETAILS   Add to Cart 
Gene Snipper Cas9 Null Protein with NLS (Nuclear localization sequence) M1103-50-BV 50 pmol 265 € DETAILS   Add to Cart 
Gene Snipper Cas9 Null Protein with NLS (Nuclear localization sequence) M1103-250-BV 250 pmol 450 € DETAILS   Add to Cart 
Gene Snipper Cas9 GFP Null Protein with NLS (Nuclear Localization Signal) M1286-50-BV 50 pmol 275 € DETAILS   Add to Cart 
Gene Snipper Cas9 GFP Null Protein with NLS (Nuclear Localization Signal) M1286-250-BV 250 pmol 485 € DETAILS   Add to Cart 
Gene Snipper SaCas9 Null Protein with NLS (Nuclear Localization Signal) M1283-50-BV 50 pmol 275 € DETAILS   Add to Cart 
Gene Snipper SaCas9 Null Protein with NLS (Nuclear Localization Signal) M1283-250-BV 250 pmol 485 € DETAILS   Add to Cart 
Cas9 Null Mutant Protein K040-ABM 50 pmol 130 € DETAILS   Add to Cart 
Cas9 Null Mutant Protein K140-ABM 250 pmol 310 € DETAILS   Add to Cart 
Gene Snipper Cas9 Null Protein M1100-50-BV 50 pmol 265 € DETAILS   Add to Cart 
Gene Snipper Cas9 Null Protein M1100-250-BV 250 pmol 450 € DETAILS   Add to Cart 
Gene Snipper SaCas9 Null Protein M1282-50-BV 50 pmol 275 € DETAILS   Add to Cart 
Gene Snipper SaCas9 Null Protein M1282-250-BV 250 pmol 485 € DETAILS   Add to Cart 

BioCat Autumn Special

Autumn Special

Browse our list of promotions and purchase your products of interest at a very special rate.

50 Euro Voucher

Email Newsletter

Subscribe to the BioCat Email Newsletter and receive a 50 € voucher.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Support

Request a Catalog
Join our Mailing List
Contact us

Home
Imprint / Impressum
Top of Page Up!