CRISPRa Expressing Cell Lines

GeneHero™ CRISPRa Stable Cell Lines are mammalian cell lines stably expressing synergistic activation mediators (SAM) for the study of transcriptional activation of endogenous genes. These CRISPRa cell lines have stably integrated human codon-optimized dCas9 fused at the C-terminus of transcriptional activator VP64, and activation helper protein complex MPH (MS2-p65-HSF1).




The cell lines are monoclonal with verified dCas9-VP64/MPH genome integration and expression. Simply introduce promoter-targeting sgRNA or CRISPRa sgRNA libraries into the CRISPRa cell lines. They are ideal for the study of gene expression activation and genetic screening.



As shown in the figures above, the transcription activation of VEGFA (A), EGFR (B) and HBG (C) was measured by qPCR in the HEK293 cell line expressing dCas9-VP64 and MPH complex (Cat# SL302) with or without transcription activation. The amplification curves of CRISPRa cell lines transduced with sgRNAs are shown in green, those without sgRNA transduction are shown in blue. The transcription levels are normalized to those of GAPDH for comparison. The transcription fold changes of genes are shown (D) by comparison of cell line transduced with and without sgRNA.

 

 

Description	Cat#	Size	Price
MDA-MB-231 Cell Line stably expressing dCas9-VPH	ZMDAMB231-CVPH-GVO-CT	1 x 10e6 cells	1630 €	DETAILS	Add to Cart
Human cell line HEK293 stably expressing dCas9-VP64 and MPH complex, monoclonal (academic institutions)	SL302-GVO-GC	2 x 10^6 cells	1062 €	DETAILS	Add to Cart
Human cell line HEK293 stably expressing dCas9-VP64 and MPH complex, monoclonal (commercial entities)	SL302-C-GVO-GC	2 x 10^6 cells	2845 €	DETAILS	Add to Cart
Human cell line MCF7 stably expressing dCas9-VP64 and MPH complex, monoclonal (academic institutions)	SL304-GVO-GC	2 x 10^6 cells	1522 €	DETAILS	Add to Cart
Human cell line MCF7 stably expressing dCas9-VP64 and MPH complex, monoclonal (commercial entities)	SL304-C-GVO-GC	2 x 10^6 cells	4169 €	DETAILS	Add to Cart