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CRISPR Cas9 transEDIT-dual Genome Engineering System: Dual gRNA Target Gene Sets

transEDIT-dual Human CRISPR Arrayed Library
transEDIT-dual Human CRISPR Arrayed Library was developed using the CRoatan*
algorithm (Erard, Knott and Hannon) by employing a random-forest-based gRNA
prediction tool to create novel and superior gRNA designs. These gRNAs were then
paired in a lentiviral vector to co-express and thus further augment their potency.
A molecular barcode was incorporated in the vector to allow for downstream screening
of high throughput analysis. The dual gRNAs are provided in a single vector to be used
with Cas9 nuclease expression vectors for superior knockout efficiency with increased
specificity and sensitivity. transEDIT-dual Human CRISPR Arrayed Library targets the
protein-coding genes of the human genome and is available as target gene sets, or as
a whole genome arrayed library in 96-well plate format.

*the algorithm is named for the national forest Croatan in North Carolina, CR stands
for CRISPR.

• CRoatan algorithm gRNA designs result in superior knockout efficiency
• Two independent gRNAs in the same lentiviral vector further enhance for potent
knockout
• Unique barcode sequence identifier (25bp) – enables downstream analysis
• Sequence verified gRNAs for confidence in results
• Higher frequency of deleterious mutations with minimal off-target effects
• Efficient delivery: transfect or transduce

Vector Options and Specifications
The 2 gRNAs are expressed from a single lentiviral vector harboring:
• Two divergent U6 promoters
• 25bp identification barcode
• Illumina-adapters
• SFFV promoter
• Bicistronic ZsGreen-P2A-PuromycinR transcript
• Multiple selection markers
• Hybrid 5’LTR (TAT independent) and a SIN 3’ LTR

Vector map of the dual gRNA expression vector
Image

transEDIT-dual CRISPR Target Gene Sets
transEDIT-dual target gene sets target human genes with 3 vectors containing two gRNAs
in each vector. Each gRNA is designed using the CRoatan algorithm for superior
performance. A negative control is also included.

• Three vectors containing two gRNAs in each vector
• Negative control
• Available as bacterial glycerol stocks or viral particles
• Cas9 expression vectors can be purchased separately


Related Links

transEDIT-dual gRNA Negative and Positive Controls

 

Description Cat# Size Price    
transEDIT-dual Human CRISPR Cas9 Target Gene Set in pCLIP-dual Vector, contains 2 dual gRNA constructs, plus negative control (glycerol stocks) TEDHG1001-GVO-TRI 1 set 565 € DETAILS   Add to Cart 
transEDIT-dual Human CRISPR Cas9 Target Gene Set in pCLIP-dual Vector with inducible Cas9, contains 2 dual gRNA constructs, negative control, and choice of inducible Cas9 expression vector (glycerol stocks) TEDHG3001-GVO-TRI 1 set 1265 € DETAILS   Add to Cart 
transEDIT-dual Human CRISPR Cas9 Target Gene Set in pCLIP-dual Vector, contains 2 dual gRNA constructs, plus negative control (Lentiviral Particles: each 100 ul at >10^7 TU/ml) TEDHV1001-GVO-TRI 1 set 1595 € DETAILS   Add to Cart 
transEDIT-dual Human CRISPR Cas9 Arrayed Whole Genome Library (contains 3+ dual gRNA constructs per gene resulting in approximately 600 96-well plaes (glycerol stocks) TEDHG9000-GVO-TRI 1 collection please inquire € DETAILS   Add to Cart 

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