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CRISPR Cas9 SmartNuclease Genome Engineering System: Lentiviral Vector-based

PrecisionX Cas9 SmartNuclease Lentiviral CRISPR/Cas9 Systems

• Proven 3rd-generation lentivector expression backbone containing codon-optimized hspCas9/Nickase
• Tagged to your choice of GFP or Puro markers for generation of cell lines stably expressing Cas9
• Deliver Cas9 to difficult-to-transfect cell lines
• Easily make stable Cas9 cell lines for editing applications
• gRNA cloning & expression systems contain necessary scaffolding sequences for crRNA maturation
• Pre-linearized format for ease of cloning gRNAs
• Precise directional cloning of the gRNA insert into vector backbone
• Rapid, highly-efficient cloning with low background (appr. 99% cloning Efficiency)
• New: Multiplex gRNA Cloning Kit to clone multiple gRNAs into a single Cas9/gRNA vector (see link below)

In addition to SBI´s industry-leading, all-in-one Cas9 SmartNuclease and SmartNickase plasmid systems, we now offer validated CRISPR/Cas9 & gRNA constructs in powerful lentivector formats. The Lenti-Cas9 SmartNuclease system is ideal for targeting cell types that are difficult to transfect with plasmids, effectively expanding the range of target cells amenable for CRISPR/Cas9-based genome engineering. The Lenti-Cas9 system also provides an easy and efficient way to generate stable Cas9 editing cell lines. Lentiviral constructs are offered in two formats:

1.) An "all-in-one" format expressing Cas9 and gRNA from a single vector
2.) A two vector system with separate Cas9 and gRNA expression vectors

All of the Cas9 lentivector constructs express human-codon optimized Cas9 wild-type nuclease or mutant nickase, while the gRNA cloning/expression lentivector constructs contain a pre-made tracrRNA scaffold with gRNA cloning sites driven by your choice of H1 or U6 Pol-III promoters for robust expression in a wide range of cells. The system is designed to accommodate flexible targeting of any genomic loci in the form of N20NGG; however, other gRNA formats (e.g. N17-18NGG) can be utilized as well.

We offer pre-packaged, ready-to-infect pseudoviral particles for expression of Cas9 wild-type nucleases and mutant nickases for generation of cell lines stably expressing Cas9. Pseudoviral particles have been packaged to exacting QC standards and comes with functional titer and in-house transduction data for each production lot of virus.

For those customers wishing to package any of the Lenti-Cas9 SmartNuclease lentivectors in their own lab, the LentiStarter 2.0 kit is available, which contains all of the necessary reagents to produce high-quality virus and transduce target cells. The Kit is available as part of a bundled package with any Lenti-Cas9 vectors as well as a standalone item (Cat #LV051A-1-SBI). Please refer to the LentiStarter 2.0 product webpage for details regarding how to use the LentiStarter 2.0 Kit.

SBI has developed the Lenti-Cas9 expression system to target a wide range of applications for researchers who are interested in the following (but not limited to) research areas:

• Genome editing and engineering of difficult-to-transfect cell lines
• In vivo engineering of model organisms
• Synthetic biology applications
• Gene/Cell-based therapy
• Genome-wide functional screening


CRISPR/Cas9 All-in-one Lentivector Formats

Wildtype Cas9 and Nickase All-in-One Format Lentivectors
The all-in-one lentiviral constructs are provided pre-linearized for cloning of guide RNAs. A single sales unit contains enough reagents to perform up to 10 ligation reactions (i.e. cloning of 10 individual gRNAs).

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CRISPR/Cas9 Two-Vector Lentivector Formats (gRNA expressed separately)

Wildtype Cas9 and Nickase T2A-Puro Format Lentivectors
The Wildtype Cas9 and Nickase T2A-Puro format lentiviral constructs are provided in plasmid form or as pre-packaged lentivirus, ready to transduce your target cells (>10^7 IFUs/ml, >10^6 infectious units, 2 x 25ul aliquots) for the lentivector formats below.
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Wildtype Cas9 and Nickase Dual Promoter EF1-GFP Format Lentivectors
The Wildtype Cas9 and Nickase dual promoter EF1-GFP format lentiviral constructs are provided in plasmid form or as pre-packaged lentivirus, ready to transduce your target cells (>10^7 IFUs/ml, >10^6 infectious units, 2 x 25ul aliquots) for the lentivector formats below.
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gRNA Cloning and Expression Lentivectors
gRNA cloning and expression lentivectors are provided in a pre-linearized format for ease of cloning gRNAs. These lentivectors can be propagated when a gRNA insert has been cloned into the MCS. High-titer lentivirus can then be packaged to transduce target cells to express the gRNA to direct CRISPR/Cas9 to the genomic locus of interest.
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Related Links

LentiStarter 2.0 kit
Multiplex gRNA Cloning Kit

 

Description Cat# Size Price    
CMV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid CASLV300PA-1-SBI 10 rxns 774 € DETAILS   Add to Cart 
MSCV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid CASLV320PA-1-SBI 10 rxns 774 € DETAILS   Add to Cart 
CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNickase Lentivector Plasmid CASLV400PA-1-SBI 10 rxns 774 € DETAILS   Add to Cart 
MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNickase Lentivector Plasmid CASLV420PA-1-SBI 10 rxns 774 € DETAILS   Add to Cart 
Cas9 Nuclease Construct: CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid CASLV100PA-1-SBI 10 ug 704 € DETAILS   Add to Cart 
Cas9 Nuclease Construct plus Kit: CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Kit CASLV100PA-KIT-SBI 1 kit 1104 € DETAILS   Add to Cart 
Cas9 Nuclease Construct: CMV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles CASLV100VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
Cas9 Nickase Construct: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid CASLV200PA-1-SBI 10 ug 704 € DETAILS   Add to Cart 
Cas9 Nickase Construct plus Kit: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Kit CASLV200PA-KIT-SBI 1 kit 1104 € DETAILS   Add to Cart 
Cas9 Nickase Construct: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles CASLV200VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
Cas9 Nuclease Construct: MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid CASLV120PA-1-SBI 10 ug 704 € DETAILS   Add to Cart 
Cas9 Nuclease Construct plus Kit: MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Kit CASLV120PA-KIT-SBI 1 kit 1104 € DETAILS   Add to Cart 
Cas9 Nuclease Construct: MSCV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles CASLV120VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
Cas9 Nickase Construct: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid CASLV220PA-1-SBI 10 ug 704 € DETAILS   Add to Cart 
Cas9 Nickase Construct plus Kit: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Kit CASLV220PA-KIT-SBI 1 kit 1104 € DETAILS   Add to Cart 
Cas9 Nickase Construct: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles CASLV220VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
Cas9 Nuclease Construct: CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid CASLV105PA-1-SBI 10 ug 704 € DETAILS   Add to Cart 
Cas9 Nuclease Construct plus Kit: CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Kit CASLV105PA-KIT-SBI 1 kit 1104 € DETAILS   Add to Cart 
Cas9 Nuclease Construct: CMV-hspCas9-EF1-GFP SmartNuclease Pre-Packaged Lentiviral Particles CASLV105VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
Cas9 Nickase Construct: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid CASLV205PA-1-SBI 10 ug 704 € DETAILS   Add to Cart 
Cas9 Nickase Construct plus Kit: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Kit CASLV205PA-KIT-SBI 1 kit 1104 € DETAILS   Add to Cart 
Cas9 Nickase Construct: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles CASLV205VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
Cas9 Nuclease Construct: MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid CASLV125PA-1-SBI 10 ug 704 € DETAILS   Add to Cart 
Cas9 Nuclease Construct plus Kit: MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Kit CASLV125PA-KIT-SBI 1 kit 1104 € DETAILS   Add to Cart 
Cas9 Nuclease Construct: MSCV-hspCas9-EF1-GFP SmartNuclease Pre-Packaged Lentiviral Particles CASLV125VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
Cas9 Nickase Construct: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid CASLV225PA-1-SBI 10 ug 704 € DETAILS   Add to Cart 
Cas9 Nickase Construct plus Kit: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Kit CASLV225PA-KIT-SBI 1 kit 1104 € DETAILS   Add to Cart 
Cas9 Nickase Construct: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles CASLV225VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
EF1-Blasticidin-H1-gRNA linearized SmartNuclease Lentivector Plasmid CASLV500PA-B-SBI 10 rxns 674 € DETAILS   Add to Cart 
EF1-GFP-H1-gRNA linearized SmartNuclease Lentivector Plasmid CASLV501PA-G-SBI 10 rxns 674 € DETAILS   Add to Cart 
EF1-RFP-H1-gRNA linearized SmartNuclease Lentivector Plasmid CASLV502PA-R-SBI 10 rxns 674 € DETAILS   Add to Cart 
EF1-Blasticidin-U6-gRNA linearized SmartNuclease Lentivector Plasmid CASLV510PA-B-SBI 10 rxns 674 € DETAILS   Add to Cart 
EF1-GFP-U6-gRNA linearized SmartNuclease Lentivector Plasmid CASLV511PA-G-SBI 10 rxns 674 € DETAILS   Add to Cart 
EF1-RFP-U6-gRNA linearized SmartNuclease Lentivector Plasmid CASLV512PA-R-SBI 10 rxns 674 € DETAILS   Add to Cart 
CMV-hspCas9-T2A-Puro-H1-AAVS1 gRNA SmartNuclease Lentivector Plasmid CASLV601PA-1-SBI 10 ug 1004 € DETAILS   Add to Cart 
CMV-hspCas9-T2A-Puro-H1-AAVS1 gRNA SmartNuclease Pre-Packaged Lentiviral Particles CASLV601VA-1-GVO-SBI >10^6 IFUs, 2 x 25ul 704 € DETAILS   Add to Cart 
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] CAS9LIG-KIT-SBI 10 rxns 174 € DETAILS   Add to Cart 

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