CRISPR Cas9 SmartNuclease Genome Engineering System: Lentiviral Vector-based

PrecisionX Cas9 SmartNuclease Lentiviral CRISPR/Cas9 Systems

• Proven 3rd-generation lentivector expression backbone containing codon-optimized hspCas9/Nickase
• Tagged to your choice of GFP or Puro markers for generation of cell lines stably expressing Cas9
• Deliver Cas9 to difficult-to-transfect cell lines
• Easily make stable Cas9 cell lines for editing applications
• gRNA cloning & expression systems contain necessary scaffolding sequences for crRNA maturation
• Pre-linearized format for ease of cloning gRNAs
• Precise directional cloning of the gRNA insert into vector backbone
• Rapid, highly-efficient cloning with low background (appr. 99% cloning Efficiency)
• New: Multiplex gRNA Cloning Kit to clone multiple gRNAs into a single Cas9/gRNA vector (see link below)

In addition to SBI´s industry-leading, all-in-one Cas9 SmartNuclease and SmartNickase plasmid systems, we now offer validated CRISPR/Cas9 & gRNA constructs in powerful lentivector formats. The Lenti-Cas9 SmartNuclease system is ideal for targeting cell types that are difficult to transfect with plasmids, effectively expanding the range of target cells amenable for CRISPR/Cas9-based genome engineering. The Lenti-Cas9 system also provides an easy and efficient way to generate stable Cas9 editing cell lines. Lentiviral constructs are offered in two formats:

1.) An "all-in-one" format expressing Cas9 and gRNA from a single vector
2.) A two vector system with separate Cas9 and gRNA expression vectors

All of the Cas9 lentivector constructs express human-codon optimized Cas9 wild-type nuclease or mutant nickase, while the gRNA cloning/expression lentivector constructs contain a pre-made tracrRNA scaffold with gRNA cloning sites driven by your choice of H1 or U6 Pol-III promoters for robust expression in a wide range of cells. The system is designed to accommodate flexible targeting of any genomic loci in the form of N20NGG; however, other gRNA formats (e.g. N17-18NGG) can be utilized as well.

We offer pre-packaged, ready-to-infect pseudoviral particles for expression of Cas9 wild-type nucleases and mutant nickases for generation of cell lines stably expressing Cas9. Pseudoviral particles have been packaged to exacting QC standards and comes with functional titer and in-house transduction data for each production lot of virus.

For those customers wishing to package any of the Lenti-Cas9 SmartNuclease lentivectors in their own lab, the LentiStarter 2.0 kit is available, which contains all of the necessary reagents to produce high-quality virus and transduce target cells. The Kit is available as part of a bundled package with any Lenti-Cas9 vectors as well as a standalone item (Cat #LV051A-1-SBI). Please refer to the LentiStarter 2.0 product webpage for details regarding how to use the LentiStarter 2.0 Kit.

SBI has developed the Lenti-Cas9 expression system to target a wide range of applications for researchers who are interested in the following (but not limited to) research areas:

• Genome editing and engineering of difficult-to-transfect cell lines
• In vivo engineering of model organisms
• Synthetic biology applications
• Gene/Cell-based therapy
• Genome-wide functional screening


CRISPR/Cas9 All-in-one Lentivector Formats

Wildtype Cas9 and Nickase All-in-One Format Lentivectors
The all-in-one lentiviral constructs are provided pre-linearized for cloning of guide RNAs. A single sales unit contains enough reagents to perform up to 10 ligation reactions (i.e. cloning of 10 individual gRNAs).




CRISPR/Cas9 Two-Vector Lentivector Formats (gRNA expressed separately)

Wildtype Cas9 and Nickase T2A-Puro Format Lentivectors
The Wildtype Cas9 and Nickase T2A-Puro format lentiviral constructs are provided in plasmid form or as pre-packaged lentivirus, ready to transduce your target cells (>10^7 IFUs/ml, >10^6 infectious units, 2 x 25ul aliquots) for the lentivector formats below.


Wildtype Cas9 and Nickase Dual Promoter EF1-GFP Format Lentivectors
The Wildtype Cas9 and Nickase dual promoter EF1-GFP format lentiviral constructs are provided in plasmid form or as pre-packaged lentivirus, ready to transduce your target cells (>10^7 IFUs/ml, >10^6 infectious units, 2 x 25ul aliquots) for the lentivector formats below.


gRNA Cloning and Expression Lentivectors
gRNA cloning and expression lentivectors are provided in a pre-linearized format for ease of cloning gRNAs. These lentivectors can be propagated when a gRNA insert has been cloned into the MCS. High-titer lentivirus can then be packaged to transduce target cells to express the gRNA to direct CRISPR/Cas9 to the genomic locus of interest.

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Related Links

LentiStarter 2.0 kit
Multiplex gRNA Cloning Kit

 

Description	Cat#	Size	Price
CMV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid	CASLV300PA-1-SBI	10 rxns	774 €	DETAILS	Add to Cart
MSCV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid	CASLV320PA-1-SBI	10 rxns	774 €	DETAILS	Add to Cart
CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNickase Lentivector Plasmid	CASLV400PA-1-SBI	10 rxns	774 €	DETAILS	Add to Cart
MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNickase Lentivector Plasmid	CASLV420PA-1-SBI	10 rxns	774 €	DETAILS	Add to Cart
Cas9 Nuclease Construct: CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid	CASLV100PA-1-SBI	10 ug	704 €	DETAILS	Add to Cart
Cas9 Nuclease Construct plus Kit: CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Kit	CASLV100PA-KIT-SBI	1 kit	1104 €	DETAILS	Add to Cart
Cas9 Nuclease Construct: CMV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles	CASLV100VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
Cas9 Nickase Construct: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid	CASLV200PA-1-SBI	10 ug	704 €	DETAILS	Add to Cart
Cas9 Nickase Construct plus Kit: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Kit	CASLV200PA-KIT-SBI	1 kit	1104 €	DETAILS	Add to Cart
Cas9 Nickase Construct: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles	CASLV200VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
Cas9 Nuclease Construct: MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid	CASLV120PA-1-SBI	10 ug	704 €	DETAILS	Add to Cart
Cas9 Nuclease Construct plus Kit: MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Kit	CASLV120PA-KIT-SBI	1 kit	1104 €	DETAILS	Add to Cart
Cas9 Nuclease Construct: MSCV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles	CASLV120VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
Cas9 Nickase Construct: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid	CASLV220PA-1-SBI	10 ug	704 €	DETAILS	Add to Cart
Cas9 Nickase Construct plus Kit: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Kit	CASLV220PA-KIT-SBI	1 kit	1104 €	DETAILS	Add to Cart
Cas9 Nickase Construct: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles	CASLV220VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
Cas9 Nuclease Construct: CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid	CASLV105PA-1-SBI	10 ug	704 €	DETAILS	Add to Cart
Cas9 Nuclease Construct plus Kit: CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Kit	CASLV105PA-KIT-SBI	1 kit	1104 €	DETAILS	Add to Cart
Cas9 Nuclease Construct: CMV-hspCas9-EF1-GFP SmartNuclease Pre-Packaged Lentiviral Particles	CASLV105VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
Cas9 Nickase Construct: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid	CASLV205PA-1-SBI	10 ug	704 €	DETAILS	Add to Cart
Cas9 Nickase Construct plus Kit: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Kit	CASLV205PA-KIT-SBI	1 kit	1104 €	DETAILS	Add to Cart
Cas9 Nickase Construct: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles	CASLV205VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
Cas9 Nuclease Construct: MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid	CASLV125PA-1-SBI	10 ug	704 €	DETAILS	Add to Cart
Cas9 Nuclease Construct plus Kit: MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Kit	CASLV125PA-KIT-SBI	1 kit	1104 €	DETAILS	Add to Cart
Cas9 Nuclease Construct: MSCV-hspCas9-EF1-GFP SmartNuclease Pre-Packaged Lentiviral Particles	CASLV125VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
Cas9 Nickase Construct: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid	CASLV225PA-1-SBI	10 ug	704 €	DETAILS	Add to Cart
Cas9 Nickase Construct plus Kit: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Kit	CASLV225PA-KIT-SBI	1 kit	1104 €	DETAILS	Add to Cart
Cas9 Nickase Construct: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles	CASLV225VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
EF1-Blasticidin-H1-gRNA linearized SmartNuclease Lentivector Plasmid	CASLV500PA-B-SBI	10 rxns	674 €	DETAILS	Add to Cart
EF1-GFP-H1-gRNA linearized SmartNuclease Lentivector Plasmid	CASLV501PA-G-SBI	10 rxns	674 €	DETAILS	Add to Cart
EF1-RFP-H1-gRNA linearized SmartNuclease Lentivector Plasmid	CASLV502PA-R-SBI	10 rxns	674 €	DETAILS	Add to Cart
EF1-Blasticidin-U6-gRNA linearized SmartNuclease Lentivector Plasmid	CASLV510PA-B-SBI	10 rxns	674 €	DETAILS	Add to Cart
EF1-GFP-U6-gRNA linearized SmartNuclease Lentivector Plasmid	CASLV511PA-G-SBI	10 rxns	674 €	DETAILS	Add to Cart
EF1-RFP-U6-gRNA linearized SmartNuclease Lentivector Plasmid	CASLV512PA-R-SBI	10 rxns	674 €	DETAILS	Add to Cart
CMV-hspCas9-T2A-Puro-H1-AAVS1 gRNA SmartNuclease Lentivector Plasmid	CASLV601PA-1-SBI	10 ug	1004 €	DETAILS	Add to Cart
CMV-hspCas9-T2A-Puro-H1-AAVS1 gRNA SmartNuclease Pre-Packaged Lentiviral Particles	CASLV601VA-1-GVO-SBI	>10^6 IFUs, 2 x 25ul	704 €	DETAILS	Add to Cart
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]	CAS9LIG-KIT-SBI	10 rxns	174 €	DETAILS	Add to Cart