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CRISPR Cas9 ROSA26 Safe Harbor Targeting System

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Genome-CRISP™ CRISPR-based mouse ROSA26 safe harbor gene knockin plasmids. Top: All-in-one clone expressing both CRISPR-Cas9 nuclease and one sgRNA targeted to the mouse ROSA26 site on chromosome 6.
Bottom: Donor clone for integrating a transgene of interest into the ROSA26 locus.

Both plasmids are used to simultaneously co-transfect cells. Selection for puromycin resistance leads to knockin of the DNA sequences between the left homologous arm (HA-Left) and right homologous arm (HA-Right).
Related Links

User Manual explaining various kit options and vectors
More Product Details

 

Description Cat# Size Price    
Genome-CRISP mouse ROSA26 safe harbor gene knock-in kit SH054-GC 200 rxns 1415 € DETAILS   Add to Cart 
Genome-CRISP mouse ROSA26 safe harbor gene knock-in kit (without donor) SH050-GC 200 rxns 1215 € DETAILS   Add to Cart 
Genome-CRISP Cas9 mouse ROSA26 Safe Harbor knockin kit-Puro SH062-GC 200 rxns 1615 € DETAILS   Add to Cart 
Genome-CRISP Cas9 mouse ROSA26 Safe Harbor knockin kit-Hygro SH063-GC 200 rxns 1615 € DETAILS   Add to Cart 
Genome-CRISP Cas9 mouse ROSA26 Safe Harbor knockin kit-Neo SH064-GC 200 rxns 1615 € DETAILS   Add to Cart 
All-in-one (Cas9 + sgRNA) clone targeting mouse ROSA26 SH150-GC 10 ug 915 € DETAILS   Add to Cart 
DC-DON-SH02 expression vector for restriction enzyme cloning (ROSA26 knockin) SH250-GC 10 ug 445 € DETAILS   Add to Cart 
pEZ-SH02 expression vector for LxR recombination cloning (ROSA26 knockin) EZ013-GC 10 ug 445 € DETAILS   Add to Cart 
Cas9 ROSA26 knockin donor clone-Puro SH350-GC 10 ug 515 € DETAILS   Add to Cart 
Cas9 ROSA26 knockin donor clone-Hygro SH351-GC 10 ug 515 € DETAILS   Add to Cart 
Cas9 ROSA26 knockin donor clone-Neo SH352-GC 10 ug 515 € DETAILS   Add to Cart 
RFP ROSA26 knockin donor clone SH358-GC 10 ug 515 € DETAILS   Add to Cart 

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