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Home -> Genomics -> Genome Engineering -> CRISPR Cas9 pCAS-Guide Genome Engineering System: Vector-based

CRISPR Cas9 pCAS-Guide Genome Engineering System: Vector-based

• RNA-guided instead of protein guided as with TALENs
• pCAS-Guide all-in-one vector expressing Cas9 and for target sequence cloning
• Custom vector construction services available
• Free guide-RNA design tool

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The latest tool in genome editing CRISPR/Cas9 allows for specific genome disruption and replacement in a flexible and simple manner resulting in high specificity and low cell toxicity. The OriGene pCAS-guide Cas9 genome editing system works by co- expression of a Cas9 protein with a guide RNA expressed from the human U6 polymerase III promoter. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3´ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.

A rescue donor vector with a functional cassette (custom synthesis is available, see below) can then be inserted into the unwound DNA. The repaired genome will now express your desired sequence with or without tags.

Cas9 Genome Editing Experimental Design

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You can purchase the OriGene pCAS-Guide Cloning Kit which includes linearized pCAS-Guide Cloning Vector, Annealing Buffer, and gRNA Sequencing Primer, the pCAS-Guide Vector Supercoil Plasmid DNA incl. gRNA Sequencing Primer, negative scrambled RNA controls, as well as the Annealing Buffer and gRNA Sequencing Primer separately.
The CAS-Guide Vector is also available in versions co-expressing GFP (for tracking/sorting of transfected cells) or CD4 (for enriching transfected cells), and as a lentiviral vector.
A CAS-Guide-Nickase Vector for for gRNA cloning and expression of the Cas9 D10A Nickase mutant is also available for researchers, who prefer the paired gRNA approach which offers enhanced specificity.

T7 In Vitro Transcription Vectors to produce gRNA, Cas9 mRNA, and Cas9 D10A mRNA are also available.

We are also offering a free gRNA designing tool (see below), and the custom synthesis of pCAS-guide RNA constructs and Rescue Donor Vectors (you can select from the following premade functional cassettes:
• GFP-LoxP-PGK-Puro-LoxP
• RFP-LoxP-PGK-BSD-LoxP
• Luc-LoxP-PGK-Puro-LoxP

Please contact us for a quote.
Related Links

Contact us for free gRNA Designing Tool
Contact us for a pCAS-Guide gRNA Synthesis Service
Contact us for a rescue donor construction service
pCAS-Guide System Validation Example
SBI Cas9 SmartNuclease System using H1 Promoter for gRNA
Original Nature Review Article
Science Article: RNA-Guided Human Genome Engineering
Science Article: Multiplex Genome Engineering Using CRISPR

 

 

Description Cat# Size Price    
pCAS-Guide CRISPR Cas9 Cloning Kit (Linearized pCAS-Guide Vector, Oligo Annaling Buffer, CF3 Sequencing Primer) GE100001-OR 10 rxns 420 € DETAILS   Add to Cart 
pCAS-Guide CRISPR Cas9 Cloning Vector (Supercoil Plasmid DNA), includes CF3 Sequencing Primer GE100002-OR 10 ug 470 € DETAILS   Add to Cart 
pCAS-Scramble Negative Control: pCAS-Guide CRISPR Cas9 Vector with a scrambled sequence GE100003-OR 10 ug 370 € DETAILS   Add to Cart 
Oligo Annealing Buffer for pCAS-Guide CRISPR Cas9 Genome Engineering System GE100007-OR 0,2 ml 75 € DETAILS   Add to Cart 
CF3 Sequencing Primer to sequence the targeting gRNA cloned into pCAS-Guide CRISPR Cas9 Cloning Vector GE100008-OR 100 pmoles 55 € DETAILS   Add to Cart 
pCAS-Guide-EF1a-GFP CRISPR Cas9 Cloning Vector (Supercoil Plasmid DNA), includes CF3 Sequencing Primer GE100018-OR 10 ug 470 € DETAILS   Add to Cart 
pCAS-Scramble-EF1a-GFP Negative Control: pCAS-Guide-EF1a-GFP CRISPR Cas9 Vector with a scrambled sequence GE100021-OR 10 ug 370 € DETAILS   Add to Cart 
pCAS-Guide-EF1a-CD4 CRISPR Cas9 Cloning Vector (Supercoil Plasmid DNA), includes CF3 Sequencing Primer GE100022-OR 10 ug 470 € DETAILS   Add to Cart 
pLenti CAS-Guide CRISPR Cas9 Cloning Kit (Linearized pLentiCAS-Guide-GFP Vector, Oligo Annaling Buffer, CF3 Sequencing Primer) GE100009-OR 10 rxns 420 € DETAILS   Add to Cart 
pLenti CAS-Guide CRISPR Cas9 Cloning Vector (Supercoil Plasmid DNA), includes CF3 Sequencing Primer GE100010-OR 10 ug 520 € DETAILS   Add to Cart 
pCAS-Guide CRISPR Cas9 D10A Nickase Cloning Vector (Supercoil Plasmid DNA) GE100019-OR 10 ug 470 € DETAILS   Add to Cart 
pT7-Guide Vector (Supercoil Plasmid DNA) for Target Sequence Cloning (IVT to produce gRNA) GE100025-OR 10 ug 340 € DETAILS   Add to Cart 
pT7-Cas9 Vector (Supercoil Plasmid DNA) for IVT to produce Cas9 mRNA GE100014-OR 10 ug 340 € DETAILS   Add to Cart 
pT7-Cas9-Nickase Vector (Supercoil Plasmid DNA) for IVT to produce Cas9 D10A Nickase mRNA GE100020-OR 10 ug 340 € DETAILS   Add to Cart 
pCMV6_Entry-Cre Cre Recombinase Mammalian Expression Vector GE100017-OR 10 ug 380 € DETAILS   Add to Cart 
CRISPR Cas9 Starter Kit: HSP60 (HA-tagged) Target and Donor DNAs and pCAS-Scramble Negative Control GE100015-OR 1 set 470 € DETAILS   Add to Cart 

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