CRISPR Cas9 pCAS-Guide Genome Engineering System: Gene-specific Ready-to-Go Knockout KitsNew: CRISPR Knockout / Knockin Kits
• Turnkey solution with all components included
• Genome-wide coverage, Human & Mouse
• Knockout the endogenous gene by CRISPR
• Knockin GFP-puro downstream of the endogenous promoter for promoter studies in an authentic chromosomal setting
CRISPR/Cas9, the latest tool in genome editing, allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. The CRISPR/Cas9 genome editing system requires the co- expression of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3´end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.
Our partner OriGene provides Ready-To-Go CRISPR Knockout / Knockin Kits including two gRNA vectors and an HR Donor Vector construct.
Click DETAILS below for further information.
|Gene Specific CRISPR Cas9 Knockout Kit (includes two gene specific gRNA vectors, one gene specific donor vector with left and right homologous arms incl. puro-GFP cassette, and Scramble Negative Control)||KN2xxxx-OR||1 set||1290 €||DETAILS||Add to Cart|