CRISPR Cas9 AAVS1 Safe Harbor Targeting System

Tools for Controlled Integration
The newest innovation from SBI puts the power of the AAVS1 safe harbor site into your
hands in two different ways:


Knock-in any gene at AAVS1:

• Precise, site-specific gene integration
• Consistently robust transgene expression
• Simplified construction of isogenic cell lines
• Minimal off-target integration with promoterless puro marker
• AAVS1 gRNA/Cas9 SmartNuclease Expression Vector and three different Donor Vectors
available separately or in combination kits


Fig.1: AAVS1 Safe Harbor Targeting Vector 2.0 / Knock-In Donor Vector with EF1
Promoter driving your Gene of Interest (AAVS1-SA-puro-EF1-MCS).
Promotorless puro marker reduces off-target integration.

For further info on all vectors see individual product details.



Easily express Cas9 at AAVS1:

• Reliable, efficient genome-wide gRNA screens
• Simplified single or multiple gene knock-ins with Cas9 already active in cells
• AAVS1 Cas9 Knock-In Donor Vector available separately or in combination kit with
AAVS1 gRNA/Cas9 SmartNuclease Expression Vector
• Premade HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus
available


Fig.2: AAVS1 Safe Harbor Cas9 Knock-In Donor Vector


Fig.3: Stable AAVS1 Cas9 HEK293 Cell Line: High-expression of the Cas9 gene at the
AAVS1 locus in HEK293 cells, as determined by qPCR (left panel). A Surveyor Assay of
high expressing clones #8 and #17 shows that Cas9 is functional - arrow corresponds to
band indicative of Cas9-mediated cleavage event, which is not present in untransfected
cells.

For further vector and cell line info see individual product details.

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Description	Cat#	Size	Price
AAVS1 gRNA/Cas9 SmartNuclease Expression Vector	CAS601A-1-SBI	10 ug	1006 €	DETAILS	Add to Cart
AAVS1 Safe Harbor Targeting Vector 2.0 / Knock-In Donor Vector with EF1 Promoter driving your Gene of Interest (AAVS1-SA-puro-EF1-MCS)	GE622A-1-SBI	10 ug	1143 €	DETAILS	Add to Cart
AAVS1 Cas9-mediated Integration Kit: AAVS1 gRNA/Cas9 SmartNuclease Expression Vector plus Knock-In Donor Vector with EF1 Promoter driving your Gene of Interest plus Junction PCR Primer Mix to confirm AAVS1 integration site	GE622A-KIT-SBI	1 kit	1971 €	DETAILS	Add to Cart
AAVS1 Safe Harbor Targeting Vector 2.0 / Knock-In Donor Vector for insertion of any promoter gene combination (AAVS1-SA-puro-MCS)	GE620A-1-SBI	10 ug	1143 €	DETAILS	Add to Cart
AAVS1 Cas9-mediated Integration Kit: AAVS1 gRNA/Cas9 SmartNuclease Expression Vector plus Knock-In Donor Vector for insertion of any promoter gene combination plus Junction PCR Primer Mix to confirm AAVS1 integration site	GE620A-KIT-SBI	1 kit	1971 €	DETAILS	Add to Cart
AAVS1 Safe Harbor Targeting Vector 2.0 / GFP Reporter Knock-In Donor Vector for flexible promoter insertion driving GFP (AAVS1-SA-puro-MCS-GFP)	GE624A-1-SBI	10 ug	1143 €	DETAILS	Add to Cart
AAVS1 Cas9-mediated Integration Kit: AAVS1 gRNA/Cas9 SmartNuclease Expression Vector plus GFP Reporter Knock-In Donor Vector for flexible promoter insertion driving GFP plus Junction PCR Primer Mix to confirm AAVS1 integration site	GE624A-KIT-SBI	1 kit	1971 €	DETAILS	Add to Cart
Primer Mix for Junction PCR to Confirm AAVS1 Integration Site (compatible with GE620A-1-SBI, GE622A-1-SBI,, GE624A-1-SBI, and CAS620A-1-SBI,), 50ul at 5uM for each primer set	GE640PR-1-SBI	50 rxns	211 €	DETAILS	Add to Cart
AAVS1 Safe Harbor Cas9 Knock-In Donor Vector (AAVS1-SA-puro-EF1-hspCas9)	CAS620A-1-SBI	10 ug	1140 €	DETAILS	Add to Cart
AAVS1 Cas9-mediated Cas9 Integration Kit: AAVS1 gRNA/Cas9 SmartNuclease Expression Vector plus Cas9 Knock-In Donor Vector plus Junction PCR Primer Mix to confirm Cas9 integration site plus PCR primers to confirm Cas9 expression	CAS620A-KIT-SBI	1 kit	2072 €	DETAILS	Add to Cart
Stable Cas9 HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus	CAS630A-1-GVO-SBI	2 x 10^6 cells	1121 €	DETAILS	Add to Cart