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CRISPR Cas9 AAVS1 Safe Harbor Targeting System

Tools for Controlled Integration
The newest innovation from SBI puts the power of the AAVS1 safe harbor site into your hands in two different ways:


Knock-in any gene at AAVS1:

• Precise, site-specific gene integration
• Consistently robust transgene expression
• Simplified construction of isogenic cell lines
• Minimal off-target integration with promoterless puro marker
• AAVS1 gRNA/Cas9 SmartNuclease Expression Vector and three different Donor Vectors available separately or in combination kits

Image
Fig.1: AAVS1 Safe Harbor Targeting Vector 2.0 / Knock-In Donor Vector with EF1 Promoter driving your Gene of Interest (AAVS1-SA-puro-EF1-MCS).
Promotorless puro marker reduces off-target integration.

For further info on all vectors see individual product details.



Easily express Cas9 at AAVS1:

• Reliable, efficient genome-wide gRNA screens
• Simplified single or multiple gene knock-ins with Cas9 already active in cells
• AAVS1 Cas9 Knock-In Donor Vector available separately or in combination kit with
AAVS1 gRNA/Cas9 SmartNuclease Expression Vector
• Premade HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus available

Image
Fig.2: AAVS1 Safe Harbor Cas9 Knock-In Donor Vector

Image
Fig.3: Stable AAVS1 Cas9 HEK293 Cell Line: High-expression of the Cas9 gene at the AAVS1 locus in HEK293 cells, as determined by qPCR (left panel). A Surveyor Assay of high expressing clones #8 and #17 shows that Cas9 is functional - arrow corresponds to band indicative of Cas9-mediated cleavage event, which is not present in untransfected cells.

For further vector and cell line info see individual product details.
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Product Brochure

 

Description Cat# Size Price    
AAVS1 gRNA/Cas9 SmartNuclease Expression Vector CAS601A-1-SBI 10 ug 991 € DETAILS   Add to Cart 
AAVS1 Safe Harbor Targeting Vector 2.0 / Knock-In Donor Vector with EF1 Promoter driving your Gene of Interest (AAVS1-SA-puro-EF1-MCS) GE622A-1-SBI 10 ug 1104 € DETAILS   Add to Cart 
AAVS1 Cas9-mediated Integration Kit: AAVS1 gRNA/Cas9 SmartNuclease Expression Vector plus Knock-In Donor Vector with EF1 Promoter driving your Gene of Interest plus Junction PCR Primer Mix to confirm AAVS1 integration site GE622A-KIT-SBI 1 kit 1904 € DETAILS   Add to Cart 
AAVS1 Safe Harbor Targeting Vector 2.0 / Knock-In Donor Vector for insertion of any promoter gene combination (AAVS1-SA-puro-MCS) GE620A-1-SBI 10 ug 1104 € DETAILS   Add to Cart 
AAVS1 Cas9-mediated Integration Kit: AAVS1 gRNA/Cas9 SmartNuclease Expression Vector plus Knock-In Donor Vector for insertion of any promoter gene combination plus Junction PCR Primer Mix to confirm AAVS1 integration site GE620A-KIT-SBI 1 kit 1904 € DETAILS   Add to Cart 
AAVS1 Safe Harbor Targeting Vector 2.0 / GFP Reporter Knock-In Donor Vector for flexible promoter insertion driving GFP (AAVS1-SA-puro-MCS-GFP) GE624A-1-SBI 10 ug 1104 € DETAILS   Add to Cart 
AAVS1 Cas9-mediated Integration Kit: AAVS1 gRNA/Cas9 SmartNuclease Expression Vector plus GFP Reporter Knock-In Donor Vector for flexible promoter insertion driving GFP plus Junction PCR Primer Mix to confirm AAVS1 integration site GE624A-KIT-SBI 1 kit 1904 € DETAILS   Add to Cart 
Primer Mix for Junction PCR to Confirm AAVS1 Integration Site (compatible with GE620A-1-SBI, GE622A-1-SBI,, GE624A-1-SBI, and CAS620A-1-SBI,), 50ul at 5uM for each primer set GE640PR-1-SBI 50 rxns 204 € DETAILS   Add to Cart 
AAVS1 Safe Harbor Cas9 Knock-In Donor Vector (AAVS1-SA-puro-EF1-hspCas9) CAS620A-1-SBI 10 ug 1123 € DETAILS   Add to Cart 
AAVS1 Cas9-mediated Cas9 Integration Kit: AAVS1 gRNA/Cas9 SmartNuclease Expression Vector plus Cas9 Knock-In Donor Vector plus Junction PCR Primer Mix to confirm Cas9 integration site plus PCR primers to confirm Cas9 expression CAS620A-KIT-SBI 1 kit 2041 € DETAILS   Add to Cart 
Stable Cas9 HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus CAS630A-1-GVO-SBI 2 x 10^6 cells 1104 € DETAILS   Add to Cart 

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