Cas9 Protein with Nuclear Localization Signal
Transfection-ready Cas9 Protein: Rapid & Safe Cas9 Delivery
With transfection-ready Cas9 protein—the newest addition to SBI´s genome editing products—you can get more efficient genome editing with fewer off-target events. Simply pre-incubate Cas9 protein with gRNA and then transfect or electroporate into your cells or model system.
• Highly efficient Cas9 Protein
• Simplify delivery to cells and embryos
• Reduce off-target events
• Reduce the potential for immune response
• Perform multiplex, high-throughput studies
• Disease model generation of organisms and cell lines

The end result is better - safer Cas9 activity for applications where off-target events need to be minimized, such as:
• Genome engineering in embryos
• Disease model generation of organisms and cell lines
• In vitro transfection of cells
• In vitro cleavage assays for functional gRNA Screens
References
1. Ramakrishna, S. et al. Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Genome Res. 24, 1020–1027 (2014).
2. Wang, L. et al. Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos. Sci. Rep. 5, 17517 (2015).
3. Chen, S., Lee, B., Lee, A. Y.-F., Modzelewski, A. J. & He, L. Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes. J. Biol. Chem. (2016). doi:10.1074/jbc.M116.733154
Get the Full System
Streamline genome editing with transfectable/electroporatable Cas9 protein—
Get Cas9 protein bundled with the T7 gRNA Cloning and Production Kit
Related Links
T7 gRNA Cloning and Production Kit
More Recombinant Cas9 Nucleases and Nickases
Description | Cat# | Size | Price | ||
---|---|---|---|---|---|
Purified Cas9 Protein (NLS-Cas9-NLS) | CAS410A-1-SBI | 50 ug | 204 € | DETAILS | Add to Cart |
EGFP-labeled Cas9 Protein (NLS-Cas9-EGFP) | CAS420A-1-SBI | 50 ug | 263 € | DETAILS | Add to Cart |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit | CAS410A-KIT-SBI | 1 kit (10 rxn) | 943 € | DETAILS | Add to Cart |
Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit | CAS420A-KIT-SBI | 1 kit (10 rxn) | 979 € | DETAILS | Add to Cart |