Home -> Genomics -> Genome Engineering -> Cas9 Expressing Cell Lines

Cas9 Expressing Cell Lines

• Stable Cas9 integration
• Minimized need for co-transfection or co-transduction of sgRNAs
• Ideal for high-throughput sgRNA applications
• Consistent, high-level Cas9 expression in a uniform genetic background
• Cas9 functionally validated using the IndelCheck™ T7 Endonuclease I assay
• Compatible with GeneCopoeia Genome-CRISP™ sgRNA clones, sgRNA libraries, and donor clones

GeneCopoeia´s stable cell lines constitutively expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications with high efficiency.

The Genome-CRISP™ Cas9 stable cell lines are available pre-made in many human, mouse, and rat cell line backgrounds. The CRISPR Cas9 nuclease is stably integrated into either the human AAVS1 or mouse ROSA26 “Safe Harbor” sites, or randomly via lentiviral-mediated transduction.

The CRISPR Cas9 nuclease, in combination with single guide RNAs (sgRNAs), is widely used to create targeted genomic modifications, from gene knockouts, mutagenesis, fusion tagging, and more, in eukaryotic cells and animal models. Stable Cas9 integration ensures robust and consistent expression of the CRISPR Cas9 nuclease.


Applications

• High-throughput knockout screening with many sgRNAs, either individually or in pools.
• Ideal for drug target discovery (Figure below).
• Convenient validation of several drug target candidates.
• Applications requiring inducible genome modification.
• Validation of sgRNA cleavage activity in a fast-growing, easy-to-transfect or transduce model cell line, either prior to transfection/transduction of your cell line, or to troubleshoot sgRNAs with little or no cleavage activity.


Example applications of Cas9-expressing stable cell lines
Use of the GeneHero™ Cas9 stable cell lines with CRISPR sgRNA libraries for drug target discovery.

A. Pooled screen. Cas9-expressing cells infected with each sgRNA library pool are screened for the desired readout. Pooled cells are subjected to Sanger sequencing for individual sgRNAs, or deep sequencing to look for over- or under-representation of individual sgRNAs.

Image





Infect cell line with
pooled sgRNA library
lentiviral particles






Screen plates for
response of phenotype
of interest





PCR and/or sequence
individual sgRNA(s)











B. Knockout screen using arrayed sgRNAs. Cas9-expressing cells are infected with individual sgRNA lentiviruses. Wells are screened for the readout of interest. Individual sgRNAs corresponding to the phenotype of interest are already known without sequencing.

Image




Infect cell line in single
wells with individual
sgRNA library
lentiviral particles







Screen plates for
response of phenotype
of interest






















In addition individual Cas9 expressing Cell Lines of other suppliers are available:

Mouse Cas9 Expressing Cell Lines from Cell Biolabs
The NIH3T3/Cas9 cell line stably expresses Cas9 and Puromycin-resistance genes.

xCELLerate™ Cas9 Stable Cell Line from Genlantis
The HEK 293 cell line stably expresses Cas9 and Blasticidin-resistance genes.





Related Links

Product Line Details
Control Lentivirus expressing sgRNA

 

 

Description Cat# Size Price    
HEK293/Cas9 Cell Line AKR-5110-GVO-CB 1 x 10^6 cells 662 € DETAILS   Add to Cart 
xCELLerate HEK293 Cas9 expressing Stable Cell Line XCL-CAS9-GVO-GL 2-3 x10^6 2384 € DETAILS   Add to Cart 
HeLa/Cas9 Cell Line AKR-5111-GVO-CB 1 x 10^6 cells 662 € DETAILS   Add to Cart 
MDA-MB-231 Cell Line stably expressing Cas9 (Blast Selection) ZMDAMB231-C9-GVO-CT 1 x 10e6 cells 1696 € DETAILS   Add to Cart 
Mouse NIH3T3/Cas9 Cell Line AKR-5104-GVO-CB 1 x 10^6 cells 586 € DETAILS   Add to Cart 

BioCat Summer Special

% Special Offers

Benefit from our current promotions

SARS-CoV-2 / COVID-19

NEW! Tools for
SARS-CoV-2
Research

Electrophoresis

40% OFF BioCat Universal Agarose

DNA and RNA Purification

Bioline

Use ISOLATE II Nucleic Acid Isolation Kits for the purification of high-quality DNA and RNA.

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!