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Cas9 Expressing Cell Lines

• Stable Cas9 integration
• Minimized need for co-transfection or co-transduction of sgRNAs
• Ideal for high-throughput sgRNA applications
• Consistent, high-level Cas9 expression in a uniform genetic background
• Cas9 functionally validated using the IndelCheck™ T7 Endonuclease I assay
• Compatible with GeneCopoeia Genome-CRISP™ sgRNA clones, sgRNA libraries, and donor clones

GeneCopoeia´s stable cell lines constitutively expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications with high efficiency.

The Genome-CRISP™ Cas9 stable cell lines are available pre-made in many human, mouse, and rat cell line backgrounds. The CRISPR Cas9 nuclease is stably integrated into either the human AAVS1 or mouse ROSA26 “Safe Harbor” sites, or randomly via lentiviral-mediated transduction.

The CRISPR Cas9 nuclease, in combination with single guide RNAs (sgRNAs), is widely used to create targeted genomic modifications, from gene knockouts, mutagenesis, fusion tagging, and more, in eukaryotic cells and animal models. Stable Cas9 integration ensures robust and consistent expression of the CRISPR Cas9 nuclease.


Applications

• High-throughput knockout screening with many sgRNAs, either individually or in pools.
• Ideal for drug target discovery (Figure below).
• Convenient validation of several drug target candidates.
• Applications requiring inducible genome modification.
• Validation of sgRNA cleavage activity in a fast-growing, easy-to-transfect or transduce model cell line, either prior to transfection/transduction of your cell line, or to troubleshoot sgRNAs with little or no cleavage activity.


Example applications of Cas9-expressing stable cell lines
Use of the GeneHero™ Cas9 stable cell lines with CRISPR sgRNA libraries for drug target discovery.

A. Pooled screen. Cas9-expressing cells infected with each sgRNA library pool are screened for the desired readout. Pooled cells are subjected to Sanger sequencing for individual sgRNAs, or deep sequencing to look for over- or under-representation of individual sgRNAs.

Image





Infect cell line with
pooled sgRNA library
lentiviral particles






Screen plates for
response of phenotype
of interest





PCR and/or sequence
individual sgRNA(s)











B. Knockout screen using arrayed sgRNAs. Cas9-expressing cells are infected with individual sgRNA lentiviruses. Wells are screened for the readout of interest. Individual sgRNAs corresponding to the phenotype of interest are already known without sequencing.

Image




Infect cell line in single
wells with individual
sgRNA library
lentiviral particles







Screen plates for
response of phenotype
of interest






















In addition individual Cas9 expressing Cell Lines of other suppliers are available:

Mouse Cas9 Expressing Cell Lines from Cell Biolabs
The NIH3T3/Cas9 cell line stably expresses Cas9 and Puromycin-resistance genes.

xCELLerate™ Cas9 Stable Cell Line from Genlantis
The HEK 293 cell line stably expresses Cas9 and Blasticidin-resistance genes.





Related Links

Product Line Details

 


Product Citations

 

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Description Cat# Size Price    
Mouse NIH3T3/Cas9 Cell Line AKR-5104-GVO-CB 1 x 10^6 cells 568 € DETAILS   Add to Cart 
xCELLerate Cas9 Stable Cell Line XCL-CAS9-GVO-GL 2-3 x10^6
Special

1983 €
DETAILS   Add to Cart 
Human cell line 786-O stably expressing Cas9, randomly inserted, single clone (commercial entities) SL577-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line 786-O stably expressing Cas9, randomly inserted, single clone (academic institutions) SL577-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line HCC827 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL578-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line HCC827 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL578-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line SK-OV-3 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL580-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line SK-OV-3 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL580-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line Granta-519 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL583-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line Granta-519 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL583-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Mouse cell line 4T1 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL581-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Mouse cell line 4T1 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL581-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Mouse cell line CT26 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL582-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Mouse cell line CT26 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL582-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line 1321N1 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL576-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line 1321N1 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL576-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line A549 stably expressing CRISPR Cas9, AAVS1 inserted, single clone (academic institutions) SL504-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line A549 stably expressing CRISPR Cas9, AAVS1 inserted, single clone (commercial entities) SL504-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line AGS stably expressing CRISPR Cas9, randomly inserted, single clone (academic institutions) SL520-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line AGS stably expressing CRISPR Cas9, randomly inserted, single clone (commercial entities) SL520-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line AU-565 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL574-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line AU-565 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL574-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line BA/F3 stably expressing CRISPR Cas9, randomly inserted, single clone (academic institutions) SL567-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line BA/F3 stably expressing CRISPR Cas9, randomly inserted, single clone (commercial entities) SL567-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line BXPC-3 stably expressing CRISPR Cas9, randomly inserted, single clone (academic institutions) SL521-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line BXPC-3 stably expressing CRISPR Cas9, randomly inserted, single clone (commercial entities) SL521-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line C2C12 stably expressing CRISPR Cas9, randomly inserted, single clone (academic institutions) SL564-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line C2C12 stably expressing CRISPR Cas9, randomly inserted, single clone (commercial entities) SL564-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line C3A stably expressing Cas9, randomly inserted, single clone (academic institutions) SL543-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line C3A stably expressing Cas9, randomly inserted, single clone (commercial entities) SL543-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line CAMA-1 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL562-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line CAMA-1 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL562-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line CFPAC-1 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL540-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line CFPAC-1 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL540-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line COLO 205 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL542-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line COLO 205 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL542-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line DU145 stably expressing CRISPR Cas9, randomly inserted, single clone (academic institutions) SL548-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line DU145 stably expressing CRISPR Cas9, randomly inserted, single clone (commercial entities) SL548-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line DU4475 stably expressing CRISPR Cas9, randomly inserted, single clone (academic institutions) SL547-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line DU4475 stably expressing CRISPR Cas9, randomly inserted, single clone (commercial entities) SL547-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line H1299 stably expressing CRISPR Cas9, AAVS1 inserted, single clone (academic institutions) SL501-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line H1299 stably expressing CRISPR Cas9, AAVS1 inserted, single clone (commercial entities) SL501-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line H1975 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL529-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line H1975 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL529-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line HCC1428 stably expressing CRISPR Cas9, randomly inserted, single clone (academic institutions) SL558-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line HCC1428 stably expressing CRISPR Cas9, randomly inserted, single clone (commercial entities) SL558-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line HCC1500 stably expressing Cas9, randomly inserted, single clone (academic institutions) SL575-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line HCC1500 stably expressing Cas9, randomly inserted, single clone (commercial entities) SL575-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
Human cell line HCC38 stably expressing CRISPR Cas9, randomly inserted, single clone (academic institutions) SL563-GVO-GC 2 x 10^6 cells 1507 € DETAILS   Add to Cart 
Human cell line HCC38 stably expressing CRISPR Cas9, randomly inserted, single clone (commercial entities) SL563-C-GVO-GC 2 x 10^6 cells 4128 € DETAILS   Add to Cart 
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