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Cas9 Activity Assays

CRISPR-Test Functional Cas9 Activity Assay Kits
Would you like to know if Cas9 is active in your cells? Cellecta’s CRISPR-Test™ assay kits accurately assess Cas9 activity in your cell line using two pre-mixed, lentiviral-packaged vectors:
- one viral mix has a green fluorescent protein (GFP) lentivirus that responds to the specific Cas9 activity measured by the kit, whether nuclease, gene repression or gene activation
- the other prepared lentiviral mix contains a red fluorescent protein (RFP) marker and functions as a control

Image
CRISPRtest workflow. A test population of cells is split into two wells. The responsive viral mix is introduced to one of the wells and the control virus to the other. After some days of incubation, cells from both cultures are analyzed by FACS. The ratio of GFP to RFP in the responsive population indicates the activity of the Cas9 variant being measured.

 

 

Description Cat# Size Price    
CRISPRtest Functional Cas9 Activity Assay Kit for Human Cells (for 5 cell line pairs) CRTEST-GVO-CT 1 kit 903 € DETAILS   Add to Cart 
CRISPRuTest Functional Cas9 Activity Assay Kit for Mammalian Cells (for 5-15 cell lines) CRUTEST-GVO-CT 1 kit 1005 € DETAILS   Add to Cart 
CRISPRaTest Functional dCas9 Activator Assay Kit for Mammalian Cells (for 5-15 cell lines) CRATEST-GVO-CT 1 kit 1005 € DETAILS   Add to Cart 
CRISPRiTest Functional dCas9 Repressor Assay Kit for Mammalian Cells (for 5-15 cell lines) CRITEST-GVO-CT 1 kit 1005 € DETAILS   Add to Cart 
CRISPR Genomic Cleavage Detection Kit K1250-25-BV 25 Reactions 431 € DETAILS   Add to Cart 

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