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CartCas9 Activity Assays
CRISPR-Test Functional Cas9 Activity Assay KitsWould you like to know if Cas9 is active in your cells? Cellecta’s CRISPR-Test™ assay kits accurately assess Cas9 activity in your cell line using two pre-mixed, lentiviral-packaged vectors:
- one viral mix has a green fluorescent protein (GFP) lentivirus that responds to the specific Cas9 activity measured by the kit, whether nuclease, gene repression or gene activation
- the other prepared lentiviral mix contains a red fluorescent protein (RFP) marker and functions as a control
CRISPRtest workflow. A test population of cells is split into two wells. The responsive viral mix is introduced to one of the wells and the control virus to the other. After some days of incubation, cells from both cultures are analyzed by FACS. The ratio of GFP to RFP in the responsive population indicates the activity of the Cas9 variant being measured.
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| CRISPRtest Functional Cas9 Activity Assay Kit for Human Cells (for 5 cell line pairs) | CRTEST-GVO-CT | 1 kit | 903 € | DETAILS | Add to Cart |
| CRISPRuTest Functional Cas9 Activity Assay Kit for Mammalian Cells (for 5-15 cell lines) | CRUTEST-GVO-CT | 1 kit | 1005 € | DETAILS | Add to Cart |
| CRISPRaTest Functional dCas9 Activator Assay Kit for Mammalian Cells (for 5-15 cell lines) | CRATEST-GVO-CT | 1 kit | 1005 € | DETAILS | Add to Cart |
| CRISPRiTest Functional dCas9 Repressor Assay Kit for Mammalian Cells (for 5-15 cell lines) | CRITEST-GVO-CT | 1 kit | 1005 € | DETAILS | Add to Cart |
| CRISPR Genomic Cleavage Detection Kit | K1250-25-BV | 25 Reactions | 431 € | DETAILS | Add to Cart |
