Custom Knockdown Cell Line Engineering
Custom Knockdown Cell Lines made with Cellecta Lentiviral shRNA Constructs
You tell us the RefSeq or other target gene identifier and the cell line of interest.
Our partner Cellecta does the rest - they can make any cell into a knockdown cell using genomically integrating lentiviral shRNA vectors.
• Virtually any transcript can be knocked down in almost any mammalian cell line from nearly any cell type, including cells that are difficult to transfect and grow incl. primary cells
• Knockdown can be tetracycline-inducible or constitutive
• Cells are confirmed for knockdown of the target gene transcript by more than 70%
• shRNA integrated in the natural chromosomal environment creating a line with a typical and stable responsiveness.
Lentiviral expression vectors are the most effective vehicles for delivering genetic material to almost any mammalian cell—including non-dividing cells and to model organisms. A pseudoviral-packaged lentiviral shRNA construct can be highly efficiently transduced and integrated into virtually any mammalian cells—even the most difficult-to-transfect primary, stem, and differentiated cells.
Please check with us if your cell line of choice is in stock at Cellecta enabling them to start your project right away at the prices shown below.
When it is not in stock, you can either provide it to us and we will ship it to Cellecta at a nominal shipping charge, or Cellecta can purchase it locally and we will charge you their purchasing costs.
You can order the engineering service in a modular way:
Stable shRNA Knockout Cell Pool Construction
Let us know your target gene and Cellecta designs and makes several target-gene specific lentiviral shRNA constructs*, transduces them and assesses their knockdown effectiveness (> 70%) by qRT-PCR. The best candidates are expanded and a cell pool
of 1 x 10e6 cells is obtained. The whole process takes about 8 weeks.
*For Cellecta´s range of lentiviral shRNA- vectors which includes constitutive H1 and U6 promoter and inducible options as well as choice of different markers see link below.
Selection, Expansion, and Confirmation of Clonal Isolate from Cell Pool (Stable shRNA Knockdown Cell Line)
The cell pool is used to make stable monoclonal cultures by selecting clones, amplifying them, and reconfirming knockdown. One fully characterized stable clone will be provided.
Depending on the growth rate of your cells this process takes about 5-7 weeks.
|Stable shRNA Knockdown Cell Pool Construction (incl. screening for validated shRNA, single knockdown)||CSCL-SH-X-GVO-CT||1 x 10^6 cells||please inquire €||DETAILS||Add to Cart|
|Selection, Expansion, and Confirmation of Clonal Isolate from Cell Pool (Stable shRNA Knockdown Cell Lines)||CSCL-XX-C-GVO-CT||1 clone||please inquire €||DETAILS||Add to Cart|