Plate Based Gene Expression Profiling (Chemoluminescence Readout)

Pathway Targeted Gene Expression Profiling
• Targeted - Profile the expression of genes involved in important pathways
• Simple - Do the gene expression profiling as doing ELISA
• No expensive scanner equipment needed - luminescence plate reader is sufficient
• Highly sensitive detection - Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection using as little as 1-10 ug total RNA
• Quantitative comparison – The difference of two or more samples in gene expression can be quantitatively analyzed.

Principle of the Technology
Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. An HRP chemiluminescent substrate is added for measurement. The concentrations of cDNAs are directly proportional to the chemiluminescent intensity of the test sample.

Single Cell Arrays are available for cDNA synthesis directly from Cell Lysates
Synthesize cDNA probes through reverse transcription directly in cell lysates without RNA preparation.

Single Stem Cell cDNA Plate Array
This array (SC-0001-SO) uses a high sensitive detection method for profiling single-cell gene expression through two level amplifications: template amplification and signal amplification.


Example - Mouse Cytokine Genes cDNA Plate Array




Reference
Ma, S. et al. (2009) Endogenous Human CaMKII Inhibitory Protein Suppresses Tumor Growth by Inducing Cell Cycle Arrest and Apoptosis through Down-regulation of the Phosphatidylinositide 3-Kinase/Akt/HDM2 Pathway. JBC 284: 24773–24782.

For gene lists and user manuals see individual products.

 

 

Description	Cat#	Size	Price
Akt Pathway Regulated Genes (Human) cDNA Plate Array (24 genes) for total RNA	AP-0161-SO	4 profilings	692 €	DETAILS	Add to Cart
Apoptosis Regulating Genes (Human) cDNA Plate Array (23 genes) for total RNA	AP-0121-SO	4 profilings	692 €	DETAILS	Add to Cart
Cytokine Genes (Mouse) cDNA Plate Array (24 genes) for total RNA	AP-1141-SO	4 profilings	692 €	DETAILS	Add to Cart
Cytokine Marker Genes (Human) cDNA Plate Array (24 genes) for total RNA	AP-0204-SO	4 profilings	692 €	DETAILS	Add to Cart
HIF Pathway Regulated Genes (Human) cDNA Plate Array (23 genes) for Cell Lysates	AP-3102-SO	4 profilings	609 €	DETAILS	Add to Cart
HIF Pathway Regulated Genes (Human) cDNA Plate Array (23 genes) for total RNA	AP-0111-SO	4 profilings	692 €	DETAILS	Add to Cart
HIF Pathway Regulated Genes (Mouse) cDNA Plate Array (23 genes) for total RNA	AP-1111-SO	4 profilings	692 €	DETAILS	Add to Cart
Inflammation Related Genes (Mouse) cDNA Plate Array (23 genes) for total RNA	AP-1131-SO	4 profilings	692 €	DETAILS	Add to Cart
NFkB Pathway Regulated Genes (Human) cDNA Plate Array (23 genes) for Cell Lysates	AP-3101-SO	4 profilings	609 €	DETAILS	Add to Cart
NFkB Pathway Regulated Genes (Human) cDNA Plate Array (23 genes) for total RNA	AP-0101-SO	4 profilings	692 €	DETAILS	Add to Cart
NFkB Pathway Regulated Genes (Mouse) cDNA Plate Array (23 genes) for total RNA	AP-1101-SO	4 profilings	692 €	DETAILS	Add to Cart
p450 Pathway Genes (Human) cDNA Plate Array (24 genes) for total RNA	AP-0202-SO	4 profilings	692 €	DETAILS	Add to Cart
p450 Pathway Genes (Rat) cDNA Plate Array (23 genes) for total RNA	AP-1181-SO	4 profilings	692 €	DETAILS	Add to Cart
p53 Pathway Regulated Genes (Human) cDNA Plate Array (24 genes) for total RNA	AP-0141-SO	4 profilings	692 €	DETAILS	Add to Cart
PPARg Pathway Regulated Genes (Rat) cDNA Plate Array (23 genes) for total RNA	AP-1161-SO	4 profilings	692 €	DETAILS	Add to Cart
PPARr Pathway Regulated Genes (Human) cDNA Plate Array (23 genes) for total RNA	AP-0201-SO	4 profilings	692 €	DETAILS	Add to Cart
Rat HIF-regulated cDNA Plate Array	AP-1121-SO	4 Samples	692 €	DETAILS	Add to Cart
STAT3 Pathway Regulated Genes (Human) cDNA Plate Array (23 genes) for total RNA	AP-0151-SO	4 profilings	692 €	DETAILS	Add to Cart
Stem Cell Marker Genes (Human) cDNA Plate Array (32 genes) for total RNA	AP-0181-SO	3 profilings	692 €	DETAILS	Add to Cart
T Cell Receptor Signaling Genes (Human) cDNA Plate Array (23 genes) for total RNA	AP-0191-SO	4 profilings	692 €	DETAILS	Add to Cart
Toxicity Genes (Human) cDNA Plate Array (23 genes) for total RNA	AP-0203-SO	4 profilings	692 €	DETAILS	Add to Cart
Wnt / b-Catenin Pathway Regulated Genes (Human) cDNA Plate Array (24 genes) for total RNA	AP-0171-SO	4 profilings	692 €	DETAILS	Add to Cart
Wnt/b-Catenin Pathway Regulated Genes (Mouse) cDNA Plate Array (23 genes) for total RNA	AP-1171-SO	4 profilings	692 €	DETAILS	Add to Cart