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Purification of PCR Products (EdgeBio Quickstep 2: High Speed and High Performance)

• Quick 2-spin protocol - Start to finish in only 5 minutes
• Excellent, reproducible recoveries - 75%–99% recovery, depending on fragment size
• High purity DNA - Eliminate primers, ssDNA, proteins, salts, dNTPs and DNA labels
• Purifies almost any fragment size - 100 bp–48.5 kb
• Minimal DNA Manipulation - No time-consuming DNA binding and elution steps. Just add your sample and spin once
• Flexible formats - Single, 96- and 384-well formats

QuickStep™2 PCR Purification Systems developed by EdgeBio offer a fast and simple method to highly purify your PCR products. Purified dsDNA passes through the system, while contaminants are captured by the SOPE™ Resin and Performa® Gel Filtration matrix. Patented SOPE (Solid-Phase Oligo Protein Elimination) Resin binds and efficiently removes primers, ssDNA, enzymes and other proteins, while the Performa Gel Filtration matrix efficiently removes >99% of salts, buffers, dNTPs and other low molecular weight assay reagents. The purified PCR products can be used in many downstream applications requiring high purity such as sequencing.

Excellent DNA Recovery
Image
Left: dsDNA fragments were purified from 50 ul PCR products using QuickStep2, Brand I, and Brand Q individual columns.
Right: dsDNA fragments were purified from 50 ul PCR products using QuickStep2, Brand I, and Brand Q 96-well plates.

Comparison of PCR Purification Procedures
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Total Reaction Volumes
QuickStep 2 Cartridges: 10–50 ul
QuickStep 2 96-Well Plates: 10–100 ul
QuickStep 2 384-Well Plates: 5–10 ul

Downstream Applications
• Further Amplification
• Sequencing
• Restriction Digestion
• Cloning

 

 

Description Cat# Size Price    
QuickStep 2 PCR Purification Kit (Individual Preps) 33617-ED 36 preps 135 € DETAILS   Add to Cart 
QuickStep 2 PCR Purification Kit (Individual Preps) 92159-ED 108 preps 269 € DETAILS   Add to Cart 
QuickStep 2 96-Well PCR Purification Kit (2 plates) 61303-ED 192 preps 350 € DETAILS   Add to Cart 
QuickStep 2 96-Well PCR Purification Kit (10 plates) 97670-ED 960 preps 1428 € DETAILS   Add to Cart 
QuickStep 2 96-Well PCR Purification Kit (50 plates) 05086-ED 4800 preps please inquire € DETAILS   Add to Cart 
QuickStep 2 384-Well PCR Purification Kit (2 plates) 39231-ED 768 preps 448 € DETAILS   Add to Cart 
QuickStep 2 384-Well PCR Purification Kit (10 plates) 89815-ED 3840 preps 1843 € DETAILS   Add to Cart 
QuickStep 2 SOPE Resin (Solidphase Oligo/Protein Elimination) Resin for PCR Purification 72406-ED 0,75 ml 94 € DETAILS   Add to Cart 
QuickStep 2 SOPE Resin (Solidphase Oligo/Protein Elimination) Resin for PCR Purification 72412-ED 3,5 ml 205 € DETAILS   Add to Cart 
QuickStep 2 SOPE Resin (Solidphase Oligo/Protein Elimination) Resin for PCR Purification 72418-ED 12 ml 542 € DETAILS   Add to Cart 

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