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Circulating DNA Isolation

Plasma/Serum and Urine Circulating DNA Isolation Kits

The Norgen Cell-Free Circulating (CFC) DNA Purification Kits provide an efficient method for the purification of fragmented free-circulating DNA or total nucleic acids from variable volumes of plasma/serum or cell-free urine samples. The kits are able to isolate all sizes of circulating DNA irrespective of size or GC content, without bias. They provide an advantage over other available kits in that they do not require extension tubes for the purification of cfc DNA from large sample volumes. The purified cfc DNA are eluted in an elution solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, and sequencing.

• Isolate all sizes of cfc-DNA
• Various sample types: Plasma/Serum, Urine, CSF and other bodily fluids
• Concentrate circulating DNA into a flexible elution volume ranging from 10-100ul
• No phenol extractions nor carrier RNA
• Inhibitor-free cfc-DNA
• Versatile input volumes
• 4 different cfc DNA isolation kit sizes available for plasma/serum input:
Mini: 200 ul - 500 ul
Micro: 100 ul - 200 ul
Midi: 1ml - 4ml
Maxi: 5ml - 10ml

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Superior Recovery of Small DNA Fragments from Plasma. Different commercial kits for the purification of cell-free circulating DNA were compared. Using the Norgen Cell-Free Circulating (CFC) DNA Purification Kits (Silicon Carbide), high yields of cfc DNA of all sizes can be isolated. For DNA fragments <150 bp the Norgen kit shows the best recovery.
Data from Diefenbach RJ et al. (2018) Evaluation of commercial kits for purification of circulating free DNA. Cancer Genetics 228-229:21-27.

Cell-Free Circulating (CFC) RNA Purification Kits developed by Norgen are also available.

The EpiQuik Circulating Cell-Free DNA Isolation Kits selectively isolates small fragment cell-free circulating DNA from plasma/serum samples using an innovative magnetic beads-based size fractionation technology.

BioChain’s cfPure™ Cell Free DNA Purification Kits utilize silica-coated paramagnetic particles to purify cfDNA from less than 1 ml to greater than 10 ml of serum or plasma. The buffers were developed to ensure efficient recovery of 100 bp – 500 bp DNA fragments in order to maximize recovery of cfDNA.


For further info click DETAILS below.

Related Links

Low Abundance DNA Quantification Kit
Plasma/Serum RNA Purification Kits
Plasma/Serum RNA/DNA Purification Mini Kit
Overview plasma/serum sample preparation kits

 

 

Description Cat# Size Price    
Plasma/Serum Cell Free Circulating DNA (cfc DNA, ccf DNA) Purification Mini Kit 55100-NB 50 preps 238 € DETAILS   Add to Cart 
Plasma/Serum Cell Free Circulating DNA (cfc DNA, ccf DNA) Purification Mini Kit - Trial Size 55140-NB 4 preps 25 € DETAILS   Add to Cart 
Plasma/Serum Cell Free Circulating DNA (cfc DNA, ccf DNA) Purification Micro Kit 55500-NB 50 preps 238 € DETAILS   Add to Cart 
Plasma/Serum Cell Free Circulating DNA (cfc DNA, ccf DNA) Purification Micro Kit - Trial Size 55540-NB 4 preps please inquire € DETAILS   Add to Cart 
Plasma/Serum Cell Free Circulating DNA (cfc DNA, ccf DNA) Purification Midi Kit 55600-NB 20 preps 424 € DETAILS   Add to Cart 
Plasma/Serum Cell Free Circulating DNA (cfc DNA, ccf DNA) Purification Midi Kit - Trial Size 55640-NB 2 preps 45 € DETAILS   Add to Cart 
Plasma/Serum Cell Free Circulating DNA (cfc DNA, ccf DNA) Purification Maxi Kit 55800-NB 10 preps 322 € DETAILS   Add to Cart 
Plasma/Serum Cell Free Circulating DNA (cfc DNA, ccf DNA) Purification Maxi Kit - Trial Size 55840-NB 2 preps please inquire € DETAILS   Add to Cart 
Plasma/Serum Circulating DNA Isolation Mini Kit slurry format - for Research Use 50600-NB 50 preps 217 € DETAILS   Add to Cart 
Plasma/Serum Circulating DNA Isolation Mini Kit slurry format - CE-marked (for IVD) Dx50600-NB 50 preps 224 € DETAILS   Add to Cart 
Plasma/Serum Circulating DNA Isolation Midi Kit slurry format 51200-NB 20 preps 255 € DETAILS   Add to Cart 
Plasma/Serum Circulating DNA Isolation Maxi Kit slurry format 51300-NB 10 preps 255 € DETAILS   Add to Cart 
Plasma/Serum Circulating and Exosomal RNA and DNA Purification Kit (Slurry Format) Mini, 0.2 - 2ml sample input 53300-NB 50 preps 521 € DETAILS   Add to Cart 
Plasma/Serum Circulating and Exosomal RNA and DNA Purification Kit (Slurry Format) Maxi, 2 - 5ml sample input 53400-NB 25 preps 580 € DETAILS   Add to Cart 
Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit 56500-NB 10 preps 286 € DETAILS   Add to Cart 
Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit 56400-NB 20 preps 382 € DETAILS   Add to Cart 
Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit 56300-NB 50 preps 345 € DETAILS   Add to Cart 
Urine Cell-Free Circulating DNA Purification Mini Kit 56600-NB 50 preps 270 € DETAILS   Add to Cart 
Urine Cell-Free Circulating DNA Purification Midi Kit 56700-NB 20 preps 424 € DETAILS   Add to Cart 
Urine Cell-Free Circulating DNA Purification Maxi Kit 56800-NB 10 preps 322 € DETAILS   Add to Cart 
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit 60100-NB 10 preps 290 € DETAILS   Add to Cart 
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit 60000-NB 20 preps 391 € DETAILS   Add to Cart 
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit 59900-NB 50 preps 330 € DETAILS   Add to Cart 
cfPure Cell Free DNA Extraction Kit (magnetic bead-based, automatization-friendly) for isolating cfDNA from 100 ml human plasma/serum (100 x 1 ml or 10 x 10 ml, scalable) K5011610-BC 1 kit 496 € DETAILS   Add to Cart 
cfPure Cell Free DNA Extraction Kit (magnetic bead-based, automatization-friendly) for isolating cfDNA from 250 ml human plasma/serum (250 x 1 ml or 25 x 10 ml, scalable) K5011625-BC 1 kit 699 € DETAILS   Add to Cart 
EpiQuik Circulating Cell-Free DNA (cf DNA, cfc DNA) Isolation Easy Kit (magnetic-bead based) for isolating cfDNA from 0.5 ml Plasma/Serum P-1065-25-EP 25 preps 210 € DETAILS   Add to Cart 
EpiQuik Circulating Cell-Free DNA (cf DNA, cfc DNA) Isolation Easy Kit (magnetic-bead based) for isolating cfDNA from 0.5 ml Plasma/Serum P-1065-50-EP 50 preps 293 € DETAILS   Add to Cart 
EpiQuik Circulating Cell-Free DNA (cf DNA, cfc DNA) Isolation Kit (magnetic-bead based) for isolating cfDNA from 0.5 ml Plasma/Serum P-1064-25-EP 25 preps 210 € DETAILS   Add to Cart 
EpiQuik Circulating Cell-Free DNA (cf DNA, cfc DNA) Isolation Kit (magnetic-bead based) for isolating cfDNA from 0.5 ml Plasma/Serum P-1064-50-EP 50 preps 293 € DETAILS   Add to Cart 

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