Circulating DNA Isolation
Plasma/Serum and Urine Circulating DNA Isolation KitsThe Norgen Cell-Free Circulating (CFC) DNA Purification Kits provide an efficient method for the purification of fragmented free-circulating DNA or total nucleic acids from variable volumes of plasma/serum or cell-free urine samples. The kits are able to isolate all sizes of circulating DNA irrespective of size or GC content, without bias. They provide an advantage over other available kits in that they do not require extension tubes for the purification of cfc DNA from large sample volumes. The purified cfc DNA are eluted in an elution solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, and sequencing.
• Isolate all sizes of cfc-DNA
• Various sample types: Plasma/Serum, Urine, CSF and other bodily fluids
• Concentrate circulating DNA into a flexible elution volume ranging from 10-100ul
• No phenol extractions nor carrier RNA
• Inhibitor-free cfc-DNA
• Versatile input volumes
• 4 different cfc DNA isolation kit sizes available for plasma/serum input:
Mini: 200 ul - 500 ul
Micro: 100 ul - 200 ul
Midi: 1ml - 4ml
Maxi: 5ml - 10ml
Superior Recovery of Small DNA Fragments from Plasma. Different commercial kits for the purification of cell-free circulating DNA were compared. Using the Norgen Cell-Free Circulating (CFC) DNA Purification Kits (Silicon Carbide), high yields of cfc DNA of all sizes can be isolated. For DNA fragments <150 bp the Norgen kit shows the best recovery.
Data from Diefenbach RJ et al. (2018) Evaluation of commercial kits for purification of circulating free DNA. Cancer Genetics 228-229:21-27.
Cell-Free Circulating (CFC) RNA Purification Kits developed by Norgen are also available.
There are more kits available from other suppliers for isolation of cell-free DNA using magnetic beads (SBI and Epigentek) or silica-coated paramagnetic particles (BioChain). For details check products listed below.
Related Links
Low Abundance DNA Quantification Kit
Plasma/Serum RNA Purification Kits
Plasma/Serum RNA/DNA Purification Mini Kit
Overview plasma/serum sample preparation kits
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Mini Kit | 55100-NB | 50 preps | 360 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit - CE-marked (for IVD) | Dx55100-NB | 50 preps | 379 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Mini Kit - Trial Size | 55140-NB | 4 preps | 19 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Micro Kit | 55500-NB | 50 preps | 302 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit - CE-marked (for IVD) | Dx55500-NB | 50 preps | 314 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Midi Kit | 55600-NB | 20 preps | 449 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Midi Kit - Trial Size | 55640-NB | 2 preps | 19 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit - CE-marked (for IVD) | Dx55600-NB | 20 preps | 468 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Maxi Kit | 55800-NB | 10 preps | 281 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit - CE-marked (for IVD) | Dx55800-NB | 10 preps | 298 € | DETAILS | Add to Cart |
| Plasma/Serum Circulating DNA Isolation Mini Kit (Slurry Format) | 50600-NB | 50 preps | 248 € | DETAILS | Add to Cart |
| Plasma/Serum Circulating DNA Isolation Midi Kit (Slurry Format) | 51200-NB | 20 preps | 275 € | DETAILS | Add to Cart |
| Plasma/Serum Circulating DNA Isolation Maxi Kit (Slurry Format) | 51300-NB | 10 preps | 254 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit | 56500-NB | 10 preps | 287 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit | 56400-NB | 20 preps | 453 € | DETAILS | Add to Cart |
| Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit | 56300-NB | 50 preps | 529 € | DETAILS | Add to Cart |
| Urine Cell-Free Circulating DNA Purification Mini Kit | 56600-NB | 50 preps | 442 € | DETAILS | Add to Cart |
| Urine Cell-Free Circulating DNA Purification Midi Kit | 56700-NB | 20 preps | 469 € | DETAILS | Add to Cart |
| Urine Cell-Free Circulating DNA Purification Maxi Kit | 56800-NB | 10 preps | 298 € | DETAILS | Add to Cart |
| Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit | 60100-NB | 10 preps | 276 € | DETAILS | Add to Cart |
| Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit | 60000-NB | 20 preps | 458 € | DETAILS | Add to Cart |
| Urine Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit | 59900-NB | 50 preps | 491 € | DETAILS | Add to Cart |
| EpiQuik Circulating Cell-Free DNA (cf DNA, cfc DNA) Isolation Kit (magnetic-bead based) for isolating cfDNA from 0.5 ml Plasma/Serum | P-1064-25-EP | 25 preps | 231 € | DETAILS | Add to Cart |
| EpiQuik Circulating Cell-Free DNA (cf DNA, cfc DNA) Isolation Kit (magnetic-bead based) for isolating cfDNA from 0.5 ml Plasma/Serum | P-1064-50-EP | 50 preps | 321 € | DETAILS | Add to Cart |
| EpiQuik Circulating Cell-Free DNA (cf DNA, cfc DNA) Isolation Easy Kit (magnetic-bead based) for isolating cfDNA from 0.5 ml Plasma/Serum | P-1065-25-EP | 25 preps | 231 € | DETAILS | Add to Cart |
| EpiQuik Circulating Cell-Free DNA (cf DNA, cfc DNA) Isolation Easy Kit (magnetic-bead based) for isolating cfDNA from 0.5 ml Plasma/Serum | P-1065-50-EP | 50 preps | 321 € | DETAILS | Add to Cart |
| cfMAX cfDNA Isolation System for high yields of cfDNA | CFMAX100A-1-SBI | 1 kit (processing of 80ml serum/plasma) | 478 € | DETAILS | Add to Cart |
| cfPure V2 Cell Free DNA Extraction Kit (magnetic bead-based, automatization-friendly) for human plasma/serum | K5011610-V2-BC | 100ml sample volume | 524 € | DETAILS | Add to Cart |
| cfPure V2 Cell Free DNA Extraction Kit (magnetic bead-based, automatization-friendly) for human plasma/serum | K5011625-V2-BC | 250ml sample volume | 785 € | DETAILS | Add to Cart |
| cfPure Max V2 Cell Free DNA Extraction Kit (magnetic bead-based, automatization-friendly) for human plasma/serum, sample volumes >5ml | K5011625MA-V2-BC | 250ml sample volume | 564 € | DETAILS | Add to Cart |
| cfPure DNA Free Plasma | Z2010001-BC | 5 ml | 25 € | DETAILS | Add to Cart |
| cfPure DNA Free Plasma | Z2010002-BC | 50 ml | 345 € | DETAILS | Add to Cart |
| cfPure MAX Cell-Free DNA Extraction Kit | K5011625MA-BC | 250ml sample volume | 564 € | DETAILS | Add to Cart |
| DNA Cleared Plasma | DCP100A-1-SBI | 100 ml | 480 € | DETAILS | Add to Cart |
| Plasma/Serum cfc-DNA Advanced Purification Kit | 68000-NB | 50 preps | 1300 € | DETAILS | Add to Cart |
| Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit | 68300-NB | 50 preps | 1655 € | DETAILS | Add to Cart |
| Plasma/Serum Total cfc-Nucleic Acid Advanced Purification Kit | 68100-NB | 50 preps | 1438 € | DETAILS | Add to Cart |
