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Cold Fusion Fast Cloning System: PCR based, universal, suitable for multiple fragment cloning


Join the revolution in new cloning technologies: Experience the thrill of the chill-cloning in less than 20 minutes
• Clone any insert, at any site within any vector
• Restriction enzyme, phosphatase and ligase free system
• Powerful technology to join multiple fragments at once
• Rapid one tube reaction for 5 minutes at room temperature, then fusion on ice for 10 minutes
• Introduce or correct mutations at will in cDNAs
• Amenable to high throughput cloning applications

The Cold Fusion technology is a simple, rapid and highly efficient cloning kit. It allows you to directly clone any PCR product(s) to any linearized expression vector, at any site.
The PCR fragments can be generated by High Fidelity DNA Polymerases like BioCat´s PRECISOR Polymerase (see link below) or Taq DNA polymerase, with primers that are designed to have at least 15 bases of homology at their linear ends to where you want the product to fuse.
The linearized vector can be generated by PCR or restriction enzymes (single or double cut). One tube simple reaction format, 5 minute incubation at room temperature then 10 minutes on ice, your PCR product(s) rapidly and accurately fuse into the linearized vector in the desired orientation. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single step. The system is highly efficient, with more than 95% positive cloning rate.

How it works
The 5x Cold Fusion Master mix contains a proprietary blend of enzymes that prepare the ends of DNA fragments for sequence-directed alignment. Compatible DNA ends are then efficiently fused and produce vector clones with great accuracy.
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Cold Fusion cloning a single DNA Fragment - Capable of cloning 3´, 5´ and blunt-end fragments efficiently
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Cold Fusion cloning multiple DNA fragments - Capable of cloning multiple fragments in the order you choose
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Related Links

PRECISOR Thermostable High-Fidelity DNA Polymerase


PDF-Downloads - Will open in new browser window

Cold Fusion Quick Protocol
Cold Fusion User Manual

 


Product Citations

 

Description Cat# Size Price    
Cold Fusion Cloning Kit with Competent Cells MC010A-1-SBI 10 rxns 348 € DETAILS   Add to Cart 
Cold Fusion Cloning Kit with Competent Cells MC100A-1-SBI 20 rxns 544 € DETAILS   Add to Cart 
Cold Fusion Cloning Kit with Competent Cells MC101A-1-SBI 50 rxns 1112 € DETAILS   Add to Cart 
Cold Fusion Cloning Kit without Competent Cells MC010A-NCC-SBI 10 rxns 318 € DETAILS   Add to Cart 
Cold Fusion Cloning Kit without Competent Cells MC100A-NCC-SBI 20 rxns 494 € DETAILS   Add to Cart 
Cold Fusion Cloning Kit without Competent Cells MC101A-NCC-SBI 50 rxns 1009 € DETAILS   Add to Cart 
Cold Fusion Cloning Kit [96X] with Competent Cells, 96-well format MC096A-1-SBI 10 reactions 1960 € DETAILS   Add to Cart 

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