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Pooled Lentiviral NGS Barcode Library

Label millions of individual cells with unique sequenceable barcodes to track their proliferation and assess the diversity of their descendant populations in culture or in vivo

Cellecta’s CloneTracker™ 50M Lentiviral Barcode Library enables the tracking of individual clones derived from a population of cells. The library contains more than 50 million lentiviral constructs with different barcode sequences. When a cell population is transduced with this barcode library, the barcodes integrate into the genomic DNA of the cells. The result is a select starter founder population where almost every cell has a different, heritable, DNA-sequenceable barcode integrated into its genomic DNA.

Since the barcode is stably integrated, it is passed onto any cell progeny when genomic DNA is replicated. This feature allows researchers to track cell proliferation over the course of an experiment. For instance, it is possible to:

• Track the fate of cell clones over a course of drug treatment
• Study the effect of differentiation on hematopoietic cells
• Assess the effect of treatment on implanted tumor cell populations


Advantages of Barcodes with Precise, Known Sequences in Lentivectors
• Broad Applicability: VSV-G pseudotyped lentiviral particles efficiently transduce virtually any mammalian cells
• Easy quantitation: Library contains 50 million barcodes designed to be easily sequenced with Illumina NextSeq, HiSeq, and GAIIx NGS platforms
• Cell-tracking, simplified: Label as many as 1 million cells with unique barcodes in one experiment
• Stable Barcode Integration to Permanently Label Cells: When transduced into cells, lentiviral vectors integrate into the host cell genome
• As the host cells divide, the barcode sequences in the lentiviral vector will also replicate and be passed onto daughter cells
• Cells can be treated, grown for several passages, frozen and thawed, and the sequences within the lentiviral vector will remain in the host cell
• After selection, treatment, or differentiation, just extract genomic DNA, amplify, and sequence to identify and quantify barcodes present in the selected cell population

Seth S (2019) Pre-existing Functional Heterogeneity of Tumorigenic Compartment as the Origin of Chemoresistance in Pancreatic Tumors. Cell Reports 26, 1518–1532.
Related Links

Overview: Cell Tracking by Genetic NGS Barcode Labeling



Description Cat# Size Price    
CloneTracker 13K Lentiviral Barcode Library (ca. 13000 barcodes), packaged BC13K-V-GVO-CT 10^8 TU 4078 € DETAILS   Add to Cart 
CloneTracker 13K Lentiviral Barcode Library (ca. 13000 barcodes), plasmid version BC13K-P-CT 200 ug 7144 € DETAILS   Add to Cart 

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