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Tagged ORF Clones

Open reading frame (ORF) clones expressed with fusion peptide tags are a powerful tool for uncovering protein function. The well characterized Myc-tag and DYKDDDDK-tag (FLAG® epitope) serve as common epitopes to aid in standardizing purification, detection and analysis across multiple proteins. (Peptide sequence of the DDK tag is DYKDDDDK. FLAG® is a registered trademark of Sigma-Aldrich).

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Proven tagged expression vectors
MGC premier Tagged ORFs are available in validated tagged expression vectors with Myc-tag or DYKDDDDK -tag (FLAG epitope) as C-terminal or N-terminal fusions. Both tags are small, minimizing interference with protein function and can be used in a variety of applications for protein analysis.

• Well characterized fusion tags reduces optimization time
• Largest collection of expression-ready ORF content
• Guaranteed in-frame expression from fully sequenced ORF

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Ready-to-express tagged ORFs
MGC premier Tagged ORF expression vectors allow you to express a protein of interest with either Myc or FLAG® fusion tags. These reagents provide tools for western blot, purification, co-imunopreciptation, protein localization and FACS analysis with validated antibodies also available.


pCMV-DDK-N and pCMV-DDK-C
The pCMV-DDK mammalian expression vectors allow you to express your protein of interest fused to DYKDDDDK, which is also known as the FLAG epitope tag. Both C- and N-terminal formats of the vector are available. The DYKDDDDK or FLAG epitope is well characterized, highly immunoreactive, and thus is ideal for co-immunoprecipitation studies. It can also be easily detected via Western blot using the anti-DYKDDDDK Antibody (which recognizes the same epitope as the FLAG-tag antibody).

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Vector cartoon shows the basic elements of the pCMV-DDK expression vector. pCMV-DDK-C shown. The N-terminal fusion format is also available. For a more detailed view see the vector map on the documents page.


pCMV-Myc-N and pCMV-Myc-C
The pCMV-Myc mammalian expression vectors allow you to express a protein of interest fused to the c-Myc epitope tag. c-Myc is well-characterized and highly immunoreactive tags, and thus ideal for immunoprecipitation studies but is also easily detected via Western blot.

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Vector cartoon shows the basic elements of the pCMV-Myc expression vector. pCMV-Myc-C shown. The N-terminal fusion format is also available. For a more detailed view see the vector map on the documents page.


Use the Transomic Fetch my Gene™ search tool (see link below) to find the cDNA or ORF clone representing your gene of interest.

Myc and Flag Tag Antibodies are available from BioCat (see link below).
Related Links

Protein Tag Antibodies
Clone Search Tool
Product Line Overview


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Flyer Tagged ORFs
Product Brochure

 

 

Description Cat# Size Price    
MGC premier Tagged ORF Cloning Vector pCMV-DDK-C (empty vector) TOC1004-GVO-TRI Glycerol Stock please inquire € DETAILS   Add to Cart 
MGC premier Tagged ORF Cloning Vector pCMV-DDK-N (empty vector) TOC1003-GVO-TRI Glycerol Stock please inquire € DETAILS   Add to Cart 
MGC premier Tagged ORF Cloning Vector pCMV-Myc-C (empty vector) TOC1002-GVO-TRI Glycerol Stock please inquire € DETAILS   Add to Cart 
MGC premier Tagged ORF Cloning Vector pCMV-Myc-N (empty vector) TOC1001-GVO-TRI Glycerol Stock please inquire € DETAILS   Add to Cart 

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