BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516
Home -> Genomics -> cDNA Clones -> PrecisionShuttle System

PrecisionShuttle System

PrecisionShuttle System
To accommodate diverse tagging needs (different tag types and/or different locations), OriGene devised the novel PrecisionShuttle system to allow easy subcloning of an ORF from one tagged vector to another. The TrueORF Entry Vector pCMV6-Entry contains C-terminal tags of Myc and DDK Tag* and is the entry vector for the PrecisionShuttle system. A large panel of destination vectors is available so that you can express the ORF with different tags, with tags at different ends of the protein, or as the native, untagged protein.

The key in the PrecisionShuttle system is the utilization of two rare-cutting restriction endonucleases, Sgf I and Mlu I. The entry vector and all destination vectors have identical multiple cloning sites (MCS) so that they can exchange the inserts through simple restriction digestion and ligation. In the TrueORF Entry Vector, the ORF is flanked by Sgf I (5’-end) and Mlu I (3’-end) sites. Digestion with these two enzymes releases the untagged ORF, which can then be ligated into a destination vector digested with the same enzymes. As all destination vectors carry the ampicillin resistance marker instead of the kanamycin resistance marker, the successfully shuttled product can be easily selected with ampicillin.

Schematic of the Precision Shuttling System
Image

Over 96% of human ORFs do not have internal Sgf I or Mlu I sites and are suitable for the above strategy. For the 4% of human ORFs that contains one or both enzyme sites, two additional restriction sites in the MCS (Asc I and Rsc II) can be used to shuttle the ORF into a destination vector. When this alternate strategy is required, that information is indicated in the description of the TrueORF clone.

Compared to the commonly used Gateway system, PrecisionShuttle has several major advantages:
• The entry vector can be used for tagged expression in mammalian cells and in cell-free systems. For many applications, there is no need to shuttle the ORF to a destination vector.
• The shuttling is accomplished with a simple restriction enzyme digestion/ligation without the need for an expensive recombination specific enzyme.
• There are no intellectual property restrictions for any users.
• Large ORFs up to 18Kb can be readily transferred using the PrecisionShuttle system while ORFs larger than 4Kb are unstable in recombination-based systems.
• The PrecisionShuttle vectors precisely add the intended tag to its desired location. There is no appendage of multiple amino acids on both ends of the protein.

* Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a registered trademark of Sigma-Aldrich

 

 

1 2
Description Cat# Size Price    
pAAV-AC-GFP PrecisionShuttle AAV vector with C-terminal tGFP tag PS100090-OR 10 ug 608 € DETAILS   Add to Cart 
pAAV-AC-Myc-DDK PrecisionShuttle AAV vector with C-terminal Myc-DDK tag PS100089-OR 10 ug 608 € DETAILS   Add to Cart 
pCMV6-A-BSD PrecisionShuttle mammalian vector with blasticidin as mammalian selection marker (no tag) PS100022-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-A-EM7-BSD PrecisionShuttle mammalian vector with blasticidin as mammalian and E.coli selection marker (no tag) PS100023-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-A-GFP PrecisionShuttle mammalian vector with independent tGFP expression PS100026-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-A-Hygro PrecisionShuttle mammalian vector with hygromycin as mammalian selection marker (no tag) PS100024-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-A-Puro PrecisionShuttle mammalian vector with puromycin as mammalian selection marker (no tag) PS100025-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC PrecisionShuttle mammalian vector with neomycin as mammalian selection marker (no tag) PS100020-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-3DDK PrecisionShuttle mammalian vector with C-terminal 3XDDK tag PS100057-OR 10 ug 464 € DETAILS   Add to Cart 
pCMV6-AC-3HA PrecisionShuttle mammalian vector with C-terminal 3XHA tag PS100067-OR 10 ug 464 € DETAILS   Add to Cart 
pCMV6-AC-DDK PrecisionShuttle mammalian vector with C-terminal DDK tag PS100005-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-DDK-His PrecisionShuttle mammalian vector with C-terminal DDK-His tag PS100009-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-FC PrecisionShuttle mammalian vector with C-terminal FC tag PS100053-OR 10 ug 464 € DETAILS   Add to Cart 
pCMV6-AC-FC-S PrecisionShuttle mammalian vector with C-terminal FC and secretion signal PS100054-OR 10 ug 464 € DETAILS   Add to Cart 
pCMV6-AC-FP602 PrecisionShuttle mammalian vector with C-terminal FP602 tag PS100037-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-FP635 PrecisionShuttle mammalian vector with C-terminal FP635 tag PS100038-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-GFP PrecisionShuttle mammalian vector with C-terminal tGFP tag PS100010-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-HA PrecisionShuttle mammalian vector with C-terminal HA tag PS100004-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-HA-His PrecisionShuttle mammalian vector with C-terminal HA-His tag PS100008-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-His PrecisionShuttle mammalian vector with C-terminal His tag PS100002-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-IRES-GFP PrecisionShuttle mammalian vector with IRES-driven tGFP expression PS100027-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-IRES-GFP-Puro PrecisionShuttle Mammalian vector with IRES-GFP and puro selection PS100059-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-mBFP PrecisionShuttle mammalian vector with C-terminal mBFP PS100043-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-mCFP PrecisionShuttle mammalian vector with C-terminal mCFP PS100044-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-mGFP PrecisionShuttle mammalian vector with C-terminal mGFP PS100040-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-mKate PrecisionShuttle mammalian vector with C-terminal mKate tag PS100039-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-mRFP PrecisionShuttle mammalian vector with C-terminal mRFP PS100041-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-Myc PrecisionShuttle mammalian vector with C-terminal Myc tag PS100003-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-Myc-DDK PrecisionShuttle mammalian vector with C-terminal Myc-DDK tag PS100007-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-Myc-His PrecisionShuttle mammalian vector with C-terminal Myc-His tag PS100006-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AC-mYFP PrecisionShuttle mammalian vector with C-terminal mYFP PS100042-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-RFP PrecisionShuttle mammalian vector with C-terminal tRFP tag PS100034-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AC-YFP PrecisionShuttle mammalian vector with C-terminal tYFP tag PS100036-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-3DDK PrecisionShuttle mammalian vector with N-terminal 3XDDK tag PS100058-OR 10 ug 464 € DETAILS   Add to Cart 
pCMV6-AN-3HA PrecisionShuttle mammalian vector with N-terminal 3XHA tag PS100066-OR 10 ug 464 € DETAILS   Add to Cart 
pCMV6-AN-DDK PrecisionShuttle mammalian vector with N-terminal DDK tag PS100014-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AN-FP602 PrecisionShuttle mammalian vector with N-terminal FP602 PS100045-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-FP635 PrecisionShuttle mammalian vector with N-terminal FP635 PS100046-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-GFP PrecisionShuttle mammalian vector with N-terminal tGFP tag PS100019-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-GFP-C-His PrecisionShuttle Mammalian vector with N-GFP and C-His double tags PS100061-OR 10 ug 732 € DETAILS   Add to Cart 
pCMV6-AN-HA PrecisionShuttle mammalian vector with N-terminal HA tag PS100013-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AN-HA-His PrecisionShuttle mammalian vector with N-terminal HA-His tag PS100017-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AN-His PrecisionShuttle mammalian vector with N-terminal His tag PS100011-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AN-His-DDK PrecisionShuttle mammalian vector with N-terminal His-DDK tag PS100018-OR 10 ug 392 € DETAILS   Add to Cart 
pCMV6-AN-mBFP PrecisionShuttle mammalian vector with N-terminal mBFP PS100051-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-mCFP PrecisionShuttle mammalian vector with N-terminal mCFP PS100052-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-mGFP PrecisionShuttle mammalian vector with N-terminal mGFP PS100048-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-mKate PrecisionShuttle mammalian vector with N-terminal mKate PS100047-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-mRFP PrecisionShuttle mammalian vector with N-terminal mRFP PS100049-OR 10 ug 618 € DETAILS   Add to Cart 
pCMV6-AN-Myc PrecisionShuttle mammalian vector with N-terminal Myc tag PS100012-OR 10 ug 392 € DETAILS   Add to Cart 
1 2

DNA and RNA Purification

Bioline

Use ISOLATE II Nucleic Acid Isolation Kits for the purification of high-quality DNA and RNA.

BioCat Spring Special

Spring Special

10% OFF
3'UTR microRNA Target & Promoter Analysis

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!