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Home -> Genomics -> Capillary Electrophoresis DNA Sequencing (Sanger Sequencing) -> Dye Terminator Removal by Gel Filtration Plates

Dye Terminator Removal by Gel Filtration Plates

Performa® DTR Gel Filtration Plates for High Throughput Sanger Sequencing-Cleanup
Obtain Highest-Quality Sequencing Data

- Longest read lengths: Consistently greater than 900 bases
- Highest base call rates, highest base calling accuracy
- Lower repeat testing rate than observed with magnetic bead-based methods
- No dye blobs, no peak shouldering
- Accurately call bases close to the sequencing primer
- Obtain excellent signal strength


Perfect for High-Throughput and Automatization

- Plates can be used on various robotic pipettors (Biomek, Tecan, Eppendorf etc.) which avoids pipetting and transfer errors by manual transfer of sequencing reactions
- Consistent z-axis settings as resin column heights are extremely uniform within and between plates
- Pre-packed size-exclusion resin - no spilling or splashing


Shortest Time-to-Completion

- Ready to Use: Pre-packed with a fully hydrated gel filtration matrix
- 6 minutes total protocol time with 2 minutes hands-on time for 96 wells
- Simplest protocol with minimal manipulation
- Only 3 steps!

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• Pre-spin to remove hydrating liquid




• Add sequencing reactions


• Spin to collect purified sequencing products

- Collect purified products directly into ABI Prism® MicroAmp® Optical Reaction Plate
or comparable plates, load directly onto your Capillary Sequencing platform


Performa® DTR: The gold standard in gel filtration based dye terminator removal!

Performa DTR utilizes a fully hydrated gel filtration matrix that allows high molecular weight molecules (sequencing products) to pass through, while retaining small molecules(BigDye v3.1 or other Dye Terminators, nucleotides, primers, salts).

Performa DTR ensures removal of BigDye® Terminator v3.1 (or other Dye Terminators such as BigDye® Terminator v1.1, DYEnamic™ DTCS Quick Start Kit and Methods Development Kit), along with dNTPs, salts and other low molecular weight materials from sequencing reactions. DNA primers and fragments up to 15 bases, buffers, nucleotides labeled with biotin, isotopes and other assorted markers are also eliminated. To minimize the potential for interference with sequencing applications, no preservatives, salts, or buffers are used in the preparation of Performa DTR.



The plates are available in three formats:

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DTR V3 96-Well Short Plates for a total reaction volume of 5-15 ul (also available as kits including lids and receiver plates, which can be purchased separately as well)

DTR Ultra 96-Well Plates for a total reaction volume of 5-20 ul / for special sequencing protocols requiring high Dye Terminator volumes (also available as kits including lids and receiver plates, which can be purchased separately as well)

DTR 384-Well Plates for a total reaction volume of 5-10 ul



Performa® DTR is also available in individual spin column format for Single-Use Dye Terminator Removal, see link below


A partial plate protocol is available for 96-well plates; for the wells which are not used in the first run, please use Edge Biosystems´ adhesive plate sealers for interim storage - see product table below.
Related Links

Single-Use Dye Terminator Removal
New: Optima DTR Plates - same high performance at lower cost
Sequencing Buffer System for lowering Dye Terminator volumes


PDF-Downloads - Will open in new browser window

Product Overview Brochure: Performa® DTR

 

 

Description Cat# Size Price    
Performa DTR V3 96-Well Short Plate Kit (incl. lids and receiver plates) 63887-ED 2 x 96 preps 259 € DETAILS   Add to Cart 
Performa DTR V3 96-Well Short Plate Kit (incl. lids and receiver plates) 89939-ED 10 x 96 preps 971 € DETAILS   Add to Cart 
Performa DTR V3 96-Well Short Plates (without lids and receiver plates) 47938-ED 10 x 96 preps 930 € DETAILS   Add to Cart 
Performa DTR V3 96-Well Short Plates (without lids and receiver plates) 80808-ED 50 x 96 preps 3508 € DETAILS   Add to Cart 
Performa DTR Ultra 96-Well Plate Kit (incl. lids and receiver plates) 54789-ED 2 x 96 preps 284 € DETAILS   Add to Cart 
Performa DTR Ultra 96-Well Plate Kit (incl. lids and receiver plates) 55373-ED 10 x 96 preps 1106 € DETAILS   Add to Cart 
Performa DTR Ultra 96-Well Plates (without lids and receiver plates) 74251-ED 10 x 96 preps 986 € DETAILS   Add to Cart 
Performa DTR Ultra 96-Well Plates (without lids and receiver plates) 86077-ED 50 x 96 preps 3747 € DETAILS   Add to Cart 
96-Well and 384-well Plate Lids for Optima and Performa DTR Plates 33100-ED 100 lids 164 € DETAILS   Add to Cart 
96-Well Capillary Plates (Receiver Plates for Optima and Performa DTR Plates) 13506-ED 25 plates 159 € DETAILS   Add to Cart 
96-Well Capillary Plates (Receiver Plates for Optima and Performa DTR Plates) 90904-ED 100 plates 512 € DETAILS   Add to Cart 
Adhesive Plate Sealers (for sealing unused wells until using Optima and Performa DTR Plates a second time) 48461-ED 100 pieces 72 € DETAILS   Add to Cart 
Performa DTR 384-Well Plates 52571-ED 2 x 384 preps 370 € DETAILS   Add to Cart 
Performa DTR 384-Well Plates 90636-ED 10 x 384 preps 1476 € DETAILS   Add to Cart 

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