Viral Expression Vectors
A comprehensive set of cloning and expression lentivectors for efficient delivery of expression constructs in mammalian systems is provided. To achieve highly efficient delivery and stable expression, the lentiviral constructs can be packaged in VSV-G pseudoviral particles and transduced into a wide range of cell lines or to model organisms (mouse, rat, etc.). In comparison to traditional retroviral delivery systems, lentivectors actively integrate into genomic DNA without requiring cell replication.
Lentivirus Expression Vectors
Lentiviral cDNA expression vectors allow for the stable expression of any cDNA in a wide range of mammalian cells. Select your vector of choice from a large number of third-generation lentiviral expression constructs developed by SBI, Cell Biolabs and Cellecta featuring different promoter, reporter and selection marker options.
- Constitutive promotors (CMV, MSCV, EF1, PGK, UbC)
- Single, double and bidirectional promoters
- GFP, RFP, Puro, Neo or Hygro options
- HIV- or FIV-based
Lentivirus Inducible Expression Vectors
The lentiviral Cumate Switch Inducible System delivers extremely tight control, robust induction and a highly titratable expression switch for inducible gene and microRNA expression studies in mammalian cells.
- Zero background leakiness
- Robust induction
- Dynamic titratability
- Turn on->off->on again
AAV/Adenoviral Expression Vectors
Adenoviruses can infect a variety of mammalian cell types with high efficiency. They remain epichromosomal upon infection, so they are only suitable for transient expression. Adeno-Associated Virus (AAV) integrates into the host cell genome at a very specific site in one human chromosome. Random insertions are very rare, making it less immunogenic than adenovirus or retrovirus. Recombinant AAV vectors (rAAV) have been widely used for gene therapy and genome editing, mainly because of their broad tropism, the lack of disease associated with wild-type virus, ability to transduce both dividing and non-dividing cells, and long term transgene expression.
Adeno Associated Expression Vectors
The use of AAV for gene expression can be advantageous when immunogenicity is a concern. A variety of complete AAV expression systems, packaging systems and expression vectors for AAV serotypes 1 through 6 and the unique AAV-DJ and AAV-DJ/8 is provided.
Adenovirus Expression Vectors
Recombinant adenoviruses have become a popular method for protein expression because the rate of infection and the subsequent yield of recombinant protein are typically quite high.