RNA Methylation Analysis
RNA methylation occurs in different RNA species including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases. As a post-translational modification, RNA methylation plays a significant role as an epigenetic mechanism. N6-methyladenosine (m6A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes. 5-methylcytosine (5-mC) also commonly occurs in various RNA molecules. Recent data strongly suggest that m6A and 5-mC RNA methylation affects the regulation of various biological processes such as RNA stability and mRNA translation, and that abnormal RNA methylation contributes to etiology of human diseases.
RNA Methylation Analysis Kits from our partner Epigentek, see links below
*Expires 29 March 2019
For absorbance-based quantitation of N6-methyladenosine in RNA in an ELISA-like format.
For fluorometric quantitation of N6-methyladenosine (m6A or 6mA) in RNA.
For colorimetric quantitation of m6A in urine using an inhibitory competitive immunoassay method.
For measuring total m6A demethylase activity/inhibition using nuclear extracts or purified m6A demethylases like FTO and ALKBH5 from a broad range of species.
For fluorescence-based quantitation of global 5-mC RNA methylation in an ELISA-like format.
For robust, chemical-based bisulfite treatment of RNA for methylated cytosine analysis.
For efficient cDNA synthesis from bisulfite-converted RNA and enriched RNA fragments.
For RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process.