RNA Methylation Analysis
RNA methylation occurs in different RNA species including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases. As a post-translational modification, RNA methylation plays a significant role as an epigenetic mechanism. N6-methyladenosine (m6A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes. 5-methylcytosine (5-mC) also commonly occurs in various RNA molecules. Recent data strongly suggest that m6A and 5-mC RNA methylation affects the regulation of various biological processes such as RNA stability and mRNA translation, and that abnormal RNA methylation contributes to etiology of human diseases.
For absorbance-based quantitation of N6-methyladenosine in RNA in an ELISA-like format.
For fluorometric quantitation of N6-methyladenosine (m6A or 6mA) in RNA.
For colorimetric quantitation of m6A in urine using an inhibitory competitive immunoassay method.
For measuring total m6A demethylase activity/inhibition using nuclear extracts or purified m6A demethylases like FTO and ALKBH5 from a broad range of species.
For fluorescence-based quantitation of global 5-mC RNA methylation in an ELISA-like format.
For robust, chemical-based bisulfite treatment of RNA for methylated cytosine analysis.
For efficient cDNA synthesis from bisulfite-converted RNA and enriched RNA fragments.
For RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process.