Captify™ Nucleosome Panels

Captify™ Nucleosome Panels provide unprecedented access to epigenetic diversity in a physiologically relevant nucleosome context

• Unique mononucleosomes assembled from recombinant human histones
• Broad end-user applications
• Biotinylated 601 nucleosome positioning sequence
• With histone post-translational modifications (PTMs)
• Lack of free DNA
• Rigorous quality control

Each panel contains single and combinatorially-modified nucleosomes, and can be purchased as focused sets (Lysine Acylation, Arginine Methylation and the Lysine-Methylation / Oncostat panels) or as one large panel (Full Nucleosome Panel). The biotin group on the DNA facilitates pull-down experiments. EpiCypher mononucleosomes do not contain free DNA which could alter assayed activities.

All nucleosomes in the panel are subjected to EpiCypher’s rigorous quality control metrics, including: ESI-TOF mass spectrometry analysis of the modified proteins; SDS-PAGE to confirm octamer composition and purity; native PAGE to confirm nucleosome assembly and lack of free DNA; and Western blot analysis of the PTM or histone mutation (if applicable).

Three ways to leverage Captify Nucleosome Panel diversity

• Interrogating chromatin reader binding specificity: see application of the technology in Nature Article!
• Screening antibody specificity / efficiency
• As controls in IP, immunoblot, and ELISA

Background References

[1] Lowary & Widom J. Mol. Biol. (1998). PMID: 9514715
[2] Weinberg et al. Nature (2019). PMID: 31485078
[3] Weinberg et al. Nat. Genet. (2021). PMID: 33986537
[4] Dilworth et al. Nat. Chem. Biol (2022). PMID: 34782742
[5] Albanese et al. ACS Chem. Biol. (2020). PMID: 31634430

*Any manuscripts mentioning dCypher products and services reference nomenclature that existed at the time of that study. These products and services are now referred to as Captify.