Best performance, value, and convenience in competent cells for cloning and expression.
Full-length cDNA Clones and ORF Clones
Extensive clone collections from our partners transOMIC and OriGene, two of the most renowned resources of premade full-length cDNA, ORF and shRNA Clones, are offered. They comprise the full Mammalian Gene Collection (MGC) plus many hard-to-obtain clones found nowhere else, e.g. certain transcript variants, difficult-to-clone genes, and many others. Use our dedicated Clone Search Engine to easily find the optimal clone for your gene of interest.
If you need support with the selection of your cDNA clones, just use our Clone Request Tool. This service is free.
PRECISOR High-Fidelity DNA Polymerase
PRECISOR High-Fidelity DNA Polymerase generates amplicons with the highest accuracy and speed, even from your most difficult templates.
Cold Fusion Cloning Technology
The Cold Fusion Cloning Kit allows you to directly clone any PCR product(s) to any linearized expression vector, at any site. It is an efficient and rapid one tube reaction, incubation at room temperature for 5 minutes is followed by 10 minutes on ice, resulting in a >95% positive cloning rate.
Cloning without Restriction Sites
Red/ET recombination is a powerful tool for DNA subcloning, e.g. from BACs, and DNA modification, overcoming the limitations of conventional methods with respect to fragment size and restriction sites.
PiggyBac Transposon System
The PiggyBac Transposon System enables genes of interest with no size limit (10-100kb) to be easily mobilized into target genomes. The integration is reversible as the transposon can be removed from the genome, footprint-free.
Enhanced Episomal Vectors (EEV) Technology
The EEV technology is based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA1) and avoids the random integration and mutagenesis challenges of viral transduction systems. EEV vectors replicate in synchrony with the host genome with each cell cycle division for sustained, nonviral and non-integrating transgene expression in vitro and in vivo. EEV cloning and expression vectors as well as reporter vectors all in constitutive and cumate-inducible formats are offered.
Cloning Kits & Competent Cells
CloneSmart Cloning Kits are designed for general purpose cloning, for shotgun, cDNA and BAC library construction, for cloning A/T rich genomes and sequences, or for cloning problematic genomes and toxic genes.
High-efficiency competent cells for different purposes including expression of toxic proteins are also offered.