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Stem Cell Differentiation Reporter System

Monitor stem cells during differentiation
• Monitor cell differentiation in real-time
• Track 5 lineages: neural, hematopoietic, myogenic, structural and signaling
• Lentivector-based reporters with cell-specific promoter
• Choice of fluorescent marker - copGFP or RFP
• Choice of selection marker - Zeo or TK

Cell differentiation can be tracked in real-time using the Differentiation Reporter Constructs. Cell- and stage-specific promoters drive the expression of a fluorescent protein and a selection marker in differentiated cells. Lentivector-based reporters for 5 different lineages including neurogenic, hematopoietic, myogenic, structural and signaling are offered. They can be used to develop new directed ifferentiation protocols and to study cell fate specification.
All Differentiation Reporter Constructs are available as lentivector plasmids or as pre-packaged lentiviral peparations. Positive and negative transduction control plasmids and pre-packaged viral particles are also available.

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Neural (Astrocytes, Neurons, Microglia, Optic nerves)
Hematopoiesis (B-cells, T-cells, and Macrophages)
Myogenesis (Cardiac, Smooth & Skeletal Muscle)
Structural (Bone, Collagen, Skin)
Signaling (Islets, Beta-cells)

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SBI Differentiation Reporter Vector.
Cell-specific promoters drive expression of the fluorescent protein and selection marker in the corresponding cell type. Different configurations with copGFP or RFP for sorting, and Zeocin resistance for positive selection or thymidin kinase for negative selection are available.

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Stem cell differentiation tracking.
Different neural lineages can be tracked using specific differentiation reporter constructs. Cell-specific promoters: DCX (Doublecortin), GFAP (Glial Fibrillary Acidic Protein), GAD67 (GAD1, Glutamic Acid Decarboxylase 1), MAP2 (Microtubule-associated Protein 2).

Reference
Ravin, R. et al. (2008) Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 3:670-80.
Publication contains online Supplemental Materials with amazing Astrocyte differentiation movies with SBI´s GFAP Reporter.

Related Links

Pluripotency Reporter Vectors
Overview: Stem Cell Related Products
All Products for Transcription Factor Activity Determination

 

 

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Description Cat# Size Price    
Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-CMV (plasmid) SR501PA-1-SBI 10 ug 559 € DETAILS   Add to Cart 
Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-CMV (pre-packaged lentiviral particles) SR501VA-1-GVO-SBI >2 x 10^6 IFUs 685 € DETAILS   Add to Cart 
Negative Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-mCMV (plasmid) SR500PA-1-SBI 10 ug 559 € DETAILS   Add to Cart 
Negative Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-mCMV (pre-packaged lentiviral particles) SR500VA-1-GVO-SBI >2 x 10^6 IFUs 685 € DETAILS   Add to Cart 
Adipocyte Specific Stem Cell Differentiation Reporter Vector (plasmid), Mouse ALBP Promoter SR10036PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Adipocyte Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse ALBP Promoter SR10036VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Astrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Human GFAP Promoter SR10015PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Astrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Mouse GFAP Promoter SR10016PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Astrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human GFAP Promoter SR10015VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Astrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse GFAP Promoter SR10016VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Astrocyte Specific Stem Cell Differentiation Reporter Vector (pre-packaged lentiviral particles), Human GFAP Promoter, Puro SR10015VA-P-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Astrocyte Specific Stem Cell Differentiation Reporter Vector (pRedZeo, prepackaged lentiviral particles), Human GFAP Promoter SR10051PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Astrocyte Specific Stem Cell Differentiation Reporter Vector (pRedZeo, prepackaged lentiviral particles), Human GFAP Promoter SR10051VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
B-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Human B29 Promoter SR1004PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
B-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Mouse B29 Promoter SR1005PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
B-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human B29 Promoter SR1004VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
B-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse B29 Promoter SR1005VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Beta cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Human Insulin Promoter SR10028PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Beta cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Insulin Promoter SR10028VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Human mlC-2v Promoter SR10011PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Human Tnnt2 Promoter SR10012PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Mouse ACTC Promoter SR10010PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Mouse Tnnt2 Promoter SR10013PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human mlC-2v Promoter SR10011VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Tnnt2 Promoter SR10012VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse ACTC Promoter SR10010VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse Tnnt2 Promoter SR10013VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Cardiomyocyte Specific Stem Cell Differentiation Reporter Vector (plasmid), SR10068PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Cardiomyocyte Specific Stem Cell Differentiation Reporter Vector (plasmid), Human ACTC Promoter SR10049PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Cardiomyocyte Specific Stem Cell Differentiation Reporter Vector (plasmid), Human NKX2.5 Promoter SR10067PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Cardiomyocyte Specific Stem Cell Differentiation Reporter Vector (pre-packaged lentiviral particles), Alpha-Actin 2, ACTA2 Promoter SR10068VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Cardiomyocyte Specific Stem Cell Differentiation Reporter Vector (pre-packaged lentiviral particles), Human mlC-2v Promoter, EF1-Neo Marker SR10011VA-N-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Cardiomyocyte Specific Stem Cell Differentiation Reporter Vector (pre-packaged lentiviral particles), Human NKX2.5 Promoter SR10067VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Cardiomyocyte Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human ACTC Promoter SR10049VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
CD8 T-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Mouse CD8 Promoter SR1006PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
CD8 T-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse CD8 Promoter SR1006VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Chondrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Mouse col2a1 Promoter SR1001PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Chondrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse col2a1 Promoter SR1001VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Epithelium Specific Stem Cell Differentiation Reporter Vector (plasmid), Human Keratin 14 Promoter SR10038PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Epithelium Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Keratin 14 Promoter SR10038VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Erythroid Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (plasmid), Human HLA-Dra Promoter SR1007PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Erythroid Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human HLA-Dra Promoter SR1007VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Islet Specific Stem Cell Differentiation Reporter Vector (plasmid), Human NGN3 Promoter SR10037PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Islet Specific Stem Cell Differentiation Reporter Vector (plasmid), Human PDX1 Promoter SR10039PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Islet Specific Stem Cell Differentiation Reporter Vector (plasmid), Mouse PDX1 Promoter SR10040PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Islet Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human NGN3 Promoter SR10037VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Islet Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human PDX1 Promoter SR10039VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Islet Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse PDX1 Promoter SR10040VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Lymphocyte Specific Stem Cell Differentiation Reporter Vector (plasmid), Human LCK Promoter SR10032PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Lymphocyte Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human LCK Promoter SR10032VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
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