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Home -> Cell Biology -> Stem Cell related Products -> Pluripotency Reporter Systems

Pluripotency Reporter Systems

Pluripotency Response Reporter Constructs
• Monitor and quantitate Oct4 and Sox2 activity
• Lentivector-based reporters with Oct4 or Sox2 response element
• Dual reporting system with both GFP and luciferase
• Optional constitutive EF1-Puro or -Neo selection cassette

The pluripotent state of your stem cells can be verified using Oct4 (CR4) or Sox2 (SRR2) conserved enhancer Pluripotency Response Reporter Constructs. These lentivector-based transcriptional reporters express copGFP and luciferase in response to Oct4 or Sox2 activity. They work for both human and mouse stem cells. Using Oct4 (CR4) or Sox2 (SRR2) Response Reporter Constructs, you can track pluripotency in living cells with GFP and quantitate activities of Oct4 or Sox2 with luciferase.

Image
SBI Pluripotency Response Reporter Vector.
Oct4 (CR4) or Sox2 (SRR2) conserved enhancer elements are cloned upstream of a minimal CMV promoter driving copGFP and luciferase expression in pluripotent cells.


Pluripotency Promoter Reporter Constructs
• Confirm and enrich for pluripotent stem cells
• Lentivector-based reporters with Oct4 or Nanog promoter
• Choice of fluorescent marker - copGFP or RFP
• Choice of selection marker - Zeocin or thymidine kinase

Pluripotency can be tracked in living cells using the Pluripotency Promoter Reporter Constructs. These lentivector-based constructs express copGFP or RFP under control of a stem cell-specific Oct4 or Nanog promoter. Both human and mouse Oct4 and Nanog Promoter Reporter Constructs are available. They can be applied for live cell imaging, sorting and selection of pluripotent cells.

Image
SBI Pluripotency Promoter Reporter Vector.
Oct4 or Nanog promoters drive expression of the fluorescent protein and selection marker in pluripotent cells. Different configurations with copGFP or RFP for sorting, and Zeocin resistance for positive selection or thymidin kinase for negative selection are available.

All Pluripotency Reporter Constructs are available as lentivector plasmids or as pre-packaged lentiviral preparations. Positive and negative transduction control plasmids and pre-packaged viral particles are also provided.

Image
Verification of the pluripotent state of stem cells.
Oct4 (CR4) or Sox2 (SRR2) Response Reporters (left) and Oct4 or Nanog Promoter Reporters (right) were used to confirm the pluripotent state of human iPS cells and human embryonic stem cells, respectively.
Related Links

Stem Cell Differentiation Reporter System
Overview: Stem Cell Related Products
All Products for Transcription Factor Activity Determination

 

 

Description Cat# Size Price    
Oct4 CR4 Pluripotency Response Reporter Vector without selection pGreenFire Oct4 CR4-mCMV (pre-packaged lentiviral particles) SR20070-VA-1-GVO-SBI >2 x 10^6 IFUs 744 € DETAILS   Add to Cart 
Oct4 CR4 Pluripotency Response Reporter Vector without selection pGreenFire Oct4 CR4-mCMV (plasmid) SR20070-PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Oct4 CR4 Pluripotency Response Reporter Vector with Neo selection pGreenFire EF1- Neo Oct4 CR4-mCMV (plasmid) SR20070-PA-N-SBI 10 ug 1677 € DETAILS   Add to Cart 
Oct4 CR4 Pluripotency Response Reporter Vector with Puro selection pGreenFire EF1- Puro Oct4 CR4-mCMV (plasmid) SR20070-PA-P-SBI 10 ug 1677 € DETAILS   Add to Cart 
Sox2 SRR2 Pluripotency Response Reporter Vector without selection pGreenFire Sox2 SRR2-mCMV (pre-packaged lentiviral particles) SR20071-VA-1-GVO-SBI >2 x 10^6 IFUs 744 € DETAILS   Add to Cart 
Sox2 SRR2 Pluripotency Response Reporter Vector with Puro selection pGreenFire EF1- Puro Sox2 SRR2-mCMV (pre-packaged lentiviral particles) SR20071-VA-P-GVO-SBI >2 x 10^6 IFUs 744 € DETAILS   Add to Cart 
Sox2 SRR2 Pluripotency Response Reporter Vector without selection pGreenFire Sox2 SRR2-mCMV (plasmid) SR20071-PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Sox2 SRR2 Pluripotency Response Reporter Vector with Neo selection pGreenFire EF1- Neo Sox2 SRR2-mCMV (plasmid) SR20071-PA-N-SBI 10 ug 1677 € DETAILS   Add to Cart 
Sox2 SRR2 Pluripotency Response Reporter Vector with Puro selection pGreenFire EF1- Puro Sox2 SRR2-mCMV (plasmid) SR20071-PA-P-SBI 10 ug 1677 € DETAILS   Add to Cart 
pGreenFire1-mCMV (pTRH1 mCMV dscGFP T2A Fluc) for cloning your own TREs or as negative transduction control (plasmid) TR010PA-1-SBI 10 ug 655 € DETAILS   Add to Cart 
pGreenFire1-mCMV (pTRH1 mCMV dscGFP T2A Fluc EF1-Neo) for cloning your own TREs or as negative transduction control (plasmid) TR010PA-N-SBI 10 ug 655 € DETAILS   Add to Cart 
pGreenFire1-mCMV (pTRH1 mCMV dscGFP T2A Fluc EF1-Puro) for cloning your own TREs or as negative transduction control (plasmid) TR010PA-P-SBI 10 ug 655 € DETAILS   Add to Cart 
pGreenFire1-mCMV (pTRH1 mCMV dscGFP T2A Fluc) for cloning your own TREs or as negative transduction control (pre-packaged) TR010VA-1-GVO-SBI >2 x 10^6 IFUs 792 € DETAILS   Add to Cart 
pGreenFire1-mCMV (pTRH1 mCMV dscGFP T2A Fluc EF1-Neo) for cloning your own TREs or as negative transduction control (pre-packaged) TR010VA-N-GVO-SBI >2 x 10^6 IFUs 792 € DETAILS   Add to Cart 
pGreenFire1-mCMV (pTRH1 mCMV dscGFP T2A Fluc EF1-Puro) for cloning your own TREs or as negative transduction control (pre-packaged) TR010VA-P-GVO-SBI >2 x 10^6 IFUs 792 € DETAILS   Add to Cart 
pGreenFire1-CMV as positive transduction control (plasmid) TR011PA-1-SBI 10 ug 655 € DETAILS   Add to Cart 
pGreenFire1-CMV as positive transduction control (pre-packaged) TR011VA-1-GVO-SBI >2 x 10^6 IFUs 792 € DETAILS   Add to Cart 
Nanog (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Human Nanog) (pre-packaged lentiviral particles) SR10030VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Nanog (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Human Nanog) (plasmid) SR10030PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Nanog (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Human Nanog) (pre-packaged lentiviral particles) SR10042VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Nanog (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Human Nanog) (plasmid) SR10042PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Nanog (Human) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Human Nanog) (pre-packaged lentiviral particles) SR10055VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Nanog (Human) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Human Nanog) (plasmid) SR10055PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Nanog (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Mouse Nanog) (pre-packaged lentiviral particles) SR10031VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Nanog (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Mouse Nanog) (plasmid) SR10031PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Nanog (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Mouse Nanog) (pre-packaged lentiviral particles) SR10044VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Nanog (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Mouse Nanog) (plasmid) SR10044PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Nanog (Mouse) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Mouse Nanog) (pre-packaged lentiviral particles) SR10056VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Nanog (Mouse) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Mouse Nanog) (plasmid) SR10056PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Oct4 (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Human Oct4) (pre-packaged lentiviral particles) SR10033VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Oct4 (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Human Oct4) (plasmid) SR10033PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Oct4 (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Human Oct4) (pre-packaged lentiviral particles) SR10043VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Oct4 (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Human Oct4) (plasmid) SR10043PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Oct4 (Human) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Human Oct4) (pre-packaged lentiviral particles) SR10053VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Oct4 (Human) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Human Oct4) (plasmid) SR10053PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Oct4 (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Mouse Oct4) (pre-packaged lentiviral particles) SR10029VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Oct4 (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Mouse Oct4) (plasmid) SR10029PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Oct4 (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Mouse Oct4) (pre-packaged lentiviral particles) SR10045VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Oct4 (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Mouse Oct4) (plasmid) SR10045PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Oct4 (Mouse) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Mouse Oct4) (pre-packaged lentiviral particles) SR10054VA-1-GVO-SBI >2 x 10^6 IFUs 763 € DETAILS   Add to Cart 
Oct4 (Mouse) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Mouse Oct4) (plasmid) SR10054PA-1-SBI 10 ug 1677 € DETAILS   Add to Cart 
Negative Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-mCMV (plasmid) SR500PA-1-SBI 10 ug 559 € DETAILS   Add to Cart 
Negative Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-mCMV (pre-packaged lentiviral particles) SR500VA-1-GVO-SBI >2 x 10^6 IFUs 685 € DETAILS   Add to Cart 
Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-CMV (plasmid) SR501PA-1-SBI 10 ug 559 € DETAILS   Add to Cart 
Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-CMV (pre-packaged lentiviral particles) SR501VA-1-GVO-SBI >2 x 10^6 IFUs 685 € DETAILS   Add to Cart 
Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pRedZeo-CMV (plasmid) SR10046PA-1-SBI 10 ug 559 € DETAILS   Add to Cart 
Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pRedZeo-CMV (pre-packaged lentiviral particles) SR10046VA-1-GVO-SBI >2 x 10^6 IFUs 702 € DETAILS   Add to Cart 
Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pRedTK-CMV(plasmid) SR10052PA-1-SBI 10 ug 559 € DETAILS   Add to Cart 
Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pRedTK-CMV (pre-packaged lentiviral particles) SR10052VA-1-GVO-SBI >2 x 10^6 IFUs 702 € DETAILS   Add to Cart 

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