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Stably Transfected Dual-labeled Cells Expressing Luciferase and Fluorescent Protein

Luciferase and GFP Dual-Labeled Cancer Cell Lines from GeneCopoeia

• Study tumor cell properties using biochemical and genetic methods in vitro.
• Monitor tumor growth, progression, and metastasis in vivo in response to a variety of environmental stimuli, drugs, and other therapeutic treatments.


In vivo monitoring of tumor growth and metastasis provides a powerful means for studying cancer properties and development of effective therapies. Mouse models created with tumor xenografts, resulting from subcutaneous or tail vein injection of cells, have long been used for such purposes. However, without a convenient means to visualize cancer progression in these animals, invasive surgical procedures are required in order to estimate the size and weight of primary and metastatic tumors, and cannot be used for early stages in tumor development. Surgery often requires sacrificing the animals, leading to the need for large numbers of individuals and increasing the cost and risk of sampling errors.

GeneCopoeia´s pre-made labeled cancer cell lines are dual-labeled with luciferase and GFP.

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Robust luciferase experssion permits highly sensitive, non-invasive detection of cancer cell growth and progression in vivo, beginning soon after injection. In addition, GFP expression is useful for in vivo tumor monitoring without the need for substrate perfusion, or for in vitro visualization and immunocytochemistry. Pre-made cell lines from various tumor types, including breast, liver, pancreas, and colon are available.

Luciferase Activity

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Figure 1. Luciferase activity of dual-labeled cancer cell lines
A: SL001 (NCI-H1299 Lung Cancer Cell Line)
B: SL003 (NCI-H1975 LungCancer Cell Line)
C: SL016 (HCC70 Breast Cancer Cell Line)
D: SL017 (MCF7 Breast Cancer Cell Line)



GFP Expression

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Figure 2. Dual-labeled cancer cell line expressing GFP
GFP fluorescence after exposure for 100 mS at 95% cell confluence using a Nikon Fluorescence Microscope
A: SL001 (NCI-H1299 Lung Cancer Cell Line)
B: SL003 (NCI-H1975 Lung Cancer Cell Line)
C: SL016 (HCC70 Breast Cancer Cell Line)
D: SL017 (MCF7 Breast Cancer Cell Line)

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