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GST Assays

Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.

GST Colorimetric Assay Kit

BioCat´s GST Colorimetric Activity Assay Kit is based upon the GST-catalyzed reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene, which has the broadest range of isozyme detectability). Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST. The GST-catalyzed formation of CDNB-GSH produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm. One unit of GST activity is defined as the amount of enzyme producing 1 mmol of CDNB-GSH conjugate/min under the conditions of the assay.

GST Fluorometric Activity Assay Kit

The GST Fluorometric Activity Assay Kit provides a simple, fluorescence-based in vitro assay for detecting the GST activity by fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces blue (ex/em = 380/461 nm) when reacts with glutathione. GST catalizes the MCB-glutathione reactions and the fluorescence levels are proportionally to the GST amounts presence in the reaction. Thus, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader.

 

 

Description Cat# Size Price    
GST Colorimetric Assay Kit K263-100-BV 100 assays 476 € DETAILS   Add to Cart 
GST Fluorometric Assay Kit K260-100-BV 100 assays 444 € DETAILS   Add to Cart 
GST Inhibitor-2 (Ethacrynic acid)) 1556-1000-BV 1 g 142 € DETAILS   Add to Cart 

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