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Mycoplasma Detection

Contaminating mycoplasma in cell culture bind to cell membranes and cause severe difficulties in doing any experiment or cellular production process related to the cell membrane, including membrane receptor interactions and membrane fusions. The very strong binding of mycoplasma to the cell membrane induces non-specific signals through cellular membrane receptors. These non-specific signals are causing fault results and lead to misinterpretation of the results. Examples are transfection, cell signaling, immune response, stem cell research, primary cell culture & many more.


References for effects of mycoplasma contamination in cell cultures

Cell Biology
Microbiological contamination in cell culture: a potential role for hydrogen peroxide vapour (HPV) , Otter, J A. Cell Biology International Volume: 32 Issue: 2 (2008-02-01) p. 326-327.

Cancer Biology
Impact of Mycoplasma hyorhinis infection on L-arginine metabolism: differential regulation of the human and murine iNOS gene Biol Chem. 2005 Oct;386(10):1055-63.

Immunology
Mycoplasma-mediated alterations of in vitro generation and functions of human dendritic cells. J Biomed Sci. 2005;12(1):31-46.

Neurobiology
Amyloid-beta peptide degradation in cell cultures by mycoplasma contaminants. Haitian Zhao , BMC Research Notes 2008, 1:38

Pathology
Production of prostaglandin E2 in monocytes stimulated in vitro by Chlamydia trachomatis, Chlamydophila pneumoniae, and Mycoplasma fermentans. Microb Pathog. 2004 Sep;37(3):155-61.

Toxicology
Falsification of tetrazolium dye (MTT) based cytotoxicity assay results due to mycoplasma contamination of cell cultures. Anticancer Res. 1999 Mar-Apr;19(2A):1245-8.


Because of severe problem of mycoplasma contamination in cell culture, reviewers of scientific journals usually ask for additional data to prove that authors got the data from mycplasma-free conditioned cell culture.

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