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Intracellular Biosensors

• Direct expression in cells
• No exogenous chemical compounds or cofactors required
• High selectivity and sensitivity

Genetically-encoded fluorescent sensors are complex chimeric proteins consisting of a fluorescent protein and a sensing polypeptide fused in a specific manner. The sensors demonstrate clearly detectable changes in fluorescence in response to corresponding cell events. They allow for the precise targeting into various subcellular compartments or animal tissues and real-time in vivo measuring of signals in natural intracellular surroundings.
Genetically-encoded fluorescent indicators for monitoring changes in intracellular concentration of calcium ions (Ca2+) and hydrogen peroxide (H2O2) as well as sensors for early detection of caspase-3 apoptosis are offered.

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