BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516
Home -> Cell Biology -> Exosomes -> Purified Exosomes from Human and Mouse Cell Lines

Purified Exosomes from Human and Mouse Cell Lines

Exosomes are 60 - 180 nm membrane vesicles secreted by most cell types in vivo and in vitro and contain distinct subsets of RNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery and functional characterization. Exosomes are nano-sized shuttles that transport signaling RNAs, lipids and proteins to other cells. Studying exosome contents are thus a "liquid biopsy" for biomarkers to gain insights into their roles in disease initiation and progression.

Applications
• Use highly purified exosomes for standards
• Utilize for protein, and RNA cargo studies
• Investigate Human Mesenchymal stem cell exosomes for activity in vivo
• Engineer the Murine dendritic cell dexosomes as RNA/protein
delivery vehicles

Preparation of the exosomes
• All cell lines are grown in exosome-depleted FBS (Exo-FBS)
• Exosomes are purified using ExoQuick-TC
• Characterized by NanoSight for size and intactness
• CD63 Western blot analysis verified
• Exosomes provided at >1x10^6 exosomes (50 ug protein)
• Exosomes are fully functional

Cancer, Stem cells and Exosomes
Tumor-secreted exosomes have intact and functional mRNAs, small RNAs, and proteins that can alter the cellular environment to favor tumor growth. Tumor metastasis involves manipulation of the tumor´s microenvironment to optimize conditions for survival and growth both locally and to begin seeding for expansion into distant sites. Potential locations for remote tumor implantation are thus prepared in advance of actual metastasis through exosomal intercellular communications. SBI has purified exosomes from numerous, model human cancer cell lines in culture to accelerate the studies of their signaling contents and how these tumor exosomes function.

Mesenchymal stem cells (MSCs) and their exosomes are the stem cell of choice for regenerative medicine and their exosomes are known to promote tissue injury repair and reduce inflammatory responses. There is great interest in exploring the cargo of MSC exosomes and engineering them for potential therapeutic delivery applications. SBI offers highly purified exosomes from human bone marrow and pre-adipocyte MSCs for advancing the research of MSC-derived exosomes. For use in animal models, murine bone marrow-derived immature dendritic cell line exosomes, called "dexosomes", are also available. These exosomes can be engineered as delivery shuttles in vivo after transfecting them with exogenous RNAs, and can even be used for shuttling some drugs to target cells.

Purified exosomes are positive for CD63
Aliquots of purified exosomes from the cell lines and from human serum were lysed with either RIPA or M-PER buffer to make exosome protein lysates. Approximately 20 ug of protein for each sample was separated on a gradient SDS-PAGE and then transferred to nitrocellulose membranes. The membranes were probed for CD63 profiles using SBI´s anti-CD63 antibody (cat# EXOAB-CD63A-1-SBI) at a 1:1,000 dilution. Bands were detected using the secondary HRP-conjugated antibody at 1:10,000 and blots imaged. All purified exosome preparations were positive, immunoreactive for CD63 with the expected, variable banding patterns common to published exosome CD63 profiles.

Image


Confirmed by NanoSight analysis: Purified exosomes are intact and bioactive
Approximately 5 ul of the purified exosomes sample was added to 995 ul of 0.2 um filtered 1X PBS (1:200 dilution). The diluted samples were incubated in a VWR 500 model ultrasonicator water bath set at 33°C for 10 minutes to ensure adequate exosome particle dispersion. The samples were diluted 1:10 then vortexed at 2.5k for 10 seconds. This eventual 1:2,000 dilution was used to gather between 1,000 to 3,000 particle tracks per sample analysis. The samples were then loaded into a NanoSight LM10HSB with a syringe pump and the sensitivity of the camera is set to auto 16 (the most sensitive auto-setting). All data were collected in triplicate. The purified exosomes displayed the expected size distribution profiles, with peak diameters between 90-110 nm and concentrations in the range expected for media exosomes at about 1x10^10 exosomes/ml.

Image
Image

 

 

Description Cat# Size Price    
Purified frozen exosomes from A549 Human non-small cell lung cancer cell line EXOP-120A-1-SBI >1x10^6 frozen exosomes / 50 ug 486 € DETAILS   Add to Cart 
Purified frozen exosomes from BC-3 Human B lymphocyte cell line EXOP-150A-1-SBI >1x10^6 frozen exosomes / 50 ug 592 € DETAILS   Add to Cart 
Purified frozen exosomes from DMS114 Human small cell lung cancer cell line EXOP-135A-1-SBI >1x10^6 frozen exosomes / 50 ug 486 € DETAILS   Add to Cart 
Purified frozen exosomes from H196 Human small cell lung cancer cell line EXOP-130A-1-SBI >1x10^6 frozen exosomes / 50 ug 486 € DETAILS   Add to Cart 
Purified frozen exosomes from H841 Human small cell lung cancer cell line EXOP-125A-1-SBI >1x10^6 frozen exosomes / 50 ug 486 € DETAILS   Add to Cart 
Purified frozen exosomes from HEK293 Human embryonic kidney cell line EXOP-110A-1-SBI >1x10^6 frozen exosomes / 50 ug 486 € DETAILS   Add to Cart 
Purified frozen exosomes from JAWSII Mouse bone marrow immature dendritic cell line EXOP-200A-1-SBI >1x10^6 frozen exosomes / 50 ug 592 € DETAILS   Add to Cart 
Purified frozen exosomes from JM1 Human T pre-B lymphoblast cell line EXOP-160A-1-SBI >1x10^6 frozen exosomes / 50 ug 592 € DETAILS   Add to Cart 
Purified frozen exosomes from Jurkat Clone E6-1 Human T lymphocyte cell line EXOP-155A-1-SBI >1x10^6 frozen exosomes / 50 ug 592 € DETAILS   Add to Cart 
Purified frozen exosomes from MCF-7 Human breast cancer, noninvasive cell line EXOP-100A-1-SBI >1x10^6 frozen exosomes / 50 ug 486 € DETAILS   Add to Cart 
Purified frozen exosomes from MDA-MB-231 Human breast cancer, aggressive/invasive/metastatic cell line EXOP-105A-1-SBI >1x10^6 frozen exosomes / 50 ug 486 € DETAILS   Add to Cart 
Purified frozen exosomes from PC-3 Human prostate cancer cells derived from metastatic cancer cell line EXOP-115A-1-SBI >1x10^6 frozen exosomes / 50 ug 486 € DETAILS   Add to Cart 
Purified frozen exosomes from PCS-500-011 Human pre-adipose derived mesenchymal stem cell EXOP-140A-1-SBI >1x10^6 frozen exosomes / 50 ug 592 € DETAILS   Add to Cart 
Purified frozen exosomes from PCS-500-012 Human bone marrow-derived mesenchymal stem cell line EXOP-145A-1-SBI >1x10^6 frozen exosomes / 50 ug 592 € DETAILS   Add to Cart 
Purified frozen exosomes from RAWS 264.7 Mouse macrophage cell line EXOP-165A-1-SBI >1x10^6 frozen exosomes / 50 ug 592 € DETAILS   Add to Cart 
Human Preadipocyte Derived Mesenchymal Stem Cell Exosomes, Frozen EXP-F100-ZB 100 ug 824 € DETAILS   Add to Cart 
Human Placental Derived Mesenchymal Stem Cell Exosomes, Frozen EXPLMSC-F100-ZB 100 ug 987 € DETAILS   Add to Cart 
Human Cord Blood Serum Exosomes, Frozen EXCBS-F100-ZB 100 ug 1040 € DETAILS   Add to Cart 
Custom Exosome Isolation from customer provided Conditioned Medium (Up to 390mls) CA-09-ZB each 649 € DETAILS   Add to Cart 

BioCat Autumn Special

Autumn Special

10% OFF Stellaris RNA FISH Probe Sets & Buffers

50 Euro Voucher

Email Newsletter

Subscribe to the BioCat Email Newsletter and receive a 50 € voucher.

Cell-based Assays

Cell-based assays

Choose from a wide variety of cell-based assays designed for greater accuracy and increased throughput.

50% OFF Fluorescent Proteins

Fluorescent Protein Vectors

Get 50% discount on the second and subsequent vectors when ordering two or more vectors with the same fluorescent protein.

Lentiviral Barcode Library

Lentiviral-based barcode library

Track clonal populations of cells over time or through different treatments using the lentiviral-based barcode library.

Support

Request a Catalog
Join our Mailing List
Contact us

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!