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Exosome Targeting

• Display proteins on exosome surfaces
• Coat exosomes with targeting ligands
• Target specific cellular destinations
• Create stable XStamp cell lines


NEW XStamp™ Pro - Next Generation of XStamp Technology
For speed production of target-specific EVs

XStamp Pro takes advantage of the same EV surface-directing protein used in our original XStamp products but skips the expression vector and the transfection step for a faster, simpler workflow. You get an already purified peptide consisting of the target-specific ScFv fused to the EV surface-directing protein—the XStamp Pro ScFv.


XStamp™ Exosome Targeting Technology

Program Exosomes with Specific Addresses
Exosomes are extracellular nanoshuttles that facilitate communication between cells and can be engineered as therapeutic shuttles to deliver biological molecules or drugs to target disease cells. SBI has developed an exosome surface display system that enables desired protein sequences to be placed efficiently on the surfaces of engineered exosomes called the "XStamp" technology. The patented XStamp technology is based upon a C-terminal fusion of the C1C2 domain from MFG-E8. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes. The technology can be used to place cellular "addresses" on exosomes that send them to specific destinations for cargo delivery.

MFG-E8 Localizes to Exosome Surfaces (C1C2 domain)

Image
Fluorescently-labeled antibodies for MFG-E8, CD58 and CD81 were used in combination for FACs analysis. The CD58 marker is a known cell surface marker that is absent on exosomes. CD81 is known to be present both in cells and exosomes. The FACs data show that MFG-E8 is exclusively detected on exosomes and not present in the cells.


The XStamp System
To take advantage of the localization of MFG-E8 on exosomes, the C1C2 domain (XStamp domain) of the protein´s gene was cloned into SBI´s MSCV-MCS-EF1-Puro lentivector and a 5´ secretion signal sequence (SS) was placed within the multiple cloning site.
Image
The protein ligand chosen to display on exosomes is cloned into the MCS and fused to the C1C2 domain. The XStamp lentivector also features a downstream EF1-Puromycin cassette for selection and stable cell line development. The lentivector constructs can be used in transient transfection expression studies as well as for packaging into lentivirus to stably transduce cells to create cellular factories producing engineered exosomes.


Technical References

Batrakova EV, Kim MS. Using exosomes, naturally-equipped nanocarriers, for drug delivery. J Control Release. 2015 Aug 1. pii: S0168-3659(15)30042-0.

Ohno S, Takanashi M, Sudo K, Ueda S, Ishikawa A, Matsuyama N, Fujita K, Mizutani T, Ohgi T, Ochiya T, Gotoh N, Kuroda M. Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells. Mol Ther. 2013 Jan;21(1):185-91.

Delcayre A, Le Pecq JB. Exosomes as novel therapeutic nanodevices. Curr Opin Mol Ther. 2006 Feb;8(1):31-8. Review.

Hartman ZC, Wei J, Glass OK, Guo H, Lei G, Yang XY, Osada T, Hobeika A, Delcayre A, Le Pecq JB, Morse MA, Clay TM, Lyerly HK. Increasing vaccine potency through exosome antigen targeting. Vaccine. 2011 Nov 21;29(50):9361-7.

Cheng L, Huang FZ, Cheng LF, Zhu YQ, Hu Q, Li L, Wei L, Chen DW. GE11-modified liposomes for non-small cell lung cancer targeting: preparation, ex vitro and in vivo evaluation. Int J Nanomedicine. 2014 Feb 12;9:921-35.

Pasqualini R, Ruoslahti E. Organ targeting in vivo using phage display peptide libraries. Nature. 1996 Mar 28;380(6572):364-6.

Estelles A, Sperinde J, Roulon T, Aguilar B, Bonner C, LePecq JB, Delcayre A. Exosome nanovesicles displaying G protein-coupled receptors for drug discovery. Int J Nanomedicine. 2007;2(4):751-60.

Pirjo Laakkonen and Kirsi Vuorinena. Homing peptides as targeted delivery vehicles. Integr. Biol., 2010,2, 326-337.

Morse MA, Garst J, Osada T, Khan S, Hobeika A, Clay TM, Valente N, Shreeniwas R, Sutton MA, Delcayre A, Hsu DH, Le Pecq JB, Lyerly HK. A phase I study of dexosome immunotherapy in patients with advanced non-small cell lung cancer. J Transl Med. 2005 Feb 21;3(1):9.

Rountree RB, et al. Exosome targeting of tumor antigens expressed by cancer vaccines can improve antigen immunogenicity and therapeutic efficacy.Cancer Res. 2011 Aug 1;71(15):5235-44.

van der Meel R, Fens MH, Vader P, van Solinge WW, Eniola-Adefeso O, Schiffelers RM. Extracellular vesicles as drug delivery systems: lessons from the liposome field. J Control Release. 2014 Dec 10;195:72-85.

Gyorgy B, Hung ME, Breakefield XO, Leonard JN. Therapeutic applications of extracellular vesicles: clinical promise and open questions. Annu Rev Pharmacol Toxicol. 2015;55:439-64.
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Brochure Exosome - Research, Products and Services

 

 

Description Cat# Size Price    
XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro XSTP710PA-1-SBI 10 ug 802 € DETAILS   Add to Cart 
XStamp BHP1 exosome brain homing peptide targeting lentivector XSTP722PA-1-SBI 10 ug 830 € DETAILS   Add to Cart 
XStamp CD40L exosome CD40 receptor targeting lentivector XSTP725PA-1-SBI 10 ug 830 € DETAILS   Add to Cart 
XStamp GE11 exosome EGFR targeting lentivector XSTP723PA-1-SBI 10 ug 830 € DETAILS   Add to Cart 
XStamp Her2 ligand exosome Her2 receptor targeting lentivector XSTP724PA-1-SBI 10 ug 830 € DETAILS   Add to Cart 
XStamp IL2 exosome IL-2 receptor targeting lentivector XSTP726PA-1-SBI 10 ug 830 € DETAILS   Add to Cart 
XStamp mlN exosome Motilin receptor GI tract targeting lentivector XSTP720PA-1-SBI 10 ug 830 € DETAILS   Add to Cart 
XStamp NCAM exosome brain targeting lentivector XSTP721PA-1-SBI 10 ug 830 € DETAILS   Add to Cart 
XStamp Pro anti-CD16 EV Targeting Kit XSTP910A-1-SBI 10 rxn 794 € DETAILS   Add to Cart 
XStamp Pro anti-HIV EV Targeting Kit XSTP905A-1-SBI 10 rxn 794 € DETAILS   Add to Cart 
XStamp Pro anti-PD-1 EV Targeting Kit XSTP915A-1-SBI 10 rxn 794 € DETAILS   Add to Cart 
XStamp Pro Streptavidin Customizable EV Targeting Kit XSTP900A-1-SBI 10 rxn 794 € DETAILS   Add to Cart 

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