Exosome Mediated RNA and DNA Delivery to Transfection-resistant Cells• Delivery to transfection-resistant cells
• Simple loading protocol
• Non-viral delivery platform
• Create stable cell lines using EV shuttles
• Human and Mouse EV shuttles available
• Boost exosome delivery with EV-Entry
EV Shuttle Kits
Working with hard-to-transfect cells? The EV Shuttle kit takes advantage of the natural ability of exosomes to be internalized and deliver functionally active biomolecules such as nucleic acids into recipient cells. Ready-to-use exosomes derived from human embryonic kidney cells (HEK 293) or murine dendritic cells (JAWS) can be transfected with nucleic acids including siRNA, miRNAs, mRNAs as well as plasmid DNA and added to a variety of hard-to-transfect cells for enhanced functional delivery. In addition, this technology can be used to create stable cell lines which is particularly useful for cells that are refractory to transfection using the piggyBac transposon system. EV shuttles also offer a significant advantage over virus-based transduction methodology because they are non-viral, easy to handle and cost effective method to deliver RNA and DNA into target cells.
For more details please refer to the specific products.
Easy exosome shuttle loading protocol
The EV-Entry reagent significantly enhances the uptake and cargo unloading into recipient cells. See performance data below. The Reagent is sold separately.
The EV-Entry System
Extracellular vesicles (EVs), including exosomes, are nanocarriers used by cells to transport RNA and protein signals and are central to intercellular communication. EVs can be added to recipient cells to deliver exogenous cargo. The EV-Entry reagent system not only enhances the rate of uptake of EVs by the recipient cells but also increases the offloading of the transported cargo into the cytoplasm of the recipient cells. EV-Entry boosts the delivery of both RNA and protein cargo. The EV-Entry system is comprised of Reagent A and B components that are mixed together with EVs or exosomes just prior to use. The protocol takes less than an hour and highly efficient at enabling EVs and exosomes to deliver cargo to recipient cells.
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|EV Shuttle Kit Human HEK293 cell exosomes||EVS105A-1-SBI||5 reactions||495 €||DETAILS||Add to Cart|
|EV Shuttle Kit Human HEK293 cell exosomes||EVS110A-1-SBI||10 reactions||840 €||DETAILS||Add to Cart|
|EV Shuttle Kit Mouse JAWS II Mouse bone marrow dendritic cell exosomes||EVS205A-1-SBI||5 reactions||495 €||DETAILS||Add to Cart|
|EV Shuttle Kit Mouse JAWS II Mouse bone marrow dendritic cell exosomes||EVS210A-1-SBI||10 reactions||840 €||DETAILS||Add to Cart|
|EV-Entry System for Exosome Delivery||EVEN105A-1-SBI||5 reactions||344 €||DETAILS||Add to Cart|
|EV-Entry System for Exosome Delivery||EVEN110A-1-SBI||10 reactions||553 €||DETAILS||Add to Cart|