Home -> Cell Biology -> Exosomes -> Exosome Mediated RNA and DNA Delivery to Transfection-resistant Cells

Exosome Mediated RNA and DNA Delivery to Transfection-resistant Cells

• Delivery to transfection-resistant cells
• Simple loading protocol
• Non-viral delivery platform
• Create stable cell lines using EV shuttles
• Human and Mouse EV shuttles available
• Boost exosome delivery with EV-Entry

EV Shuttle Kits
Working with hard-to-transfect cells? The EV Shuttle kit takes advantage of the natural ability of exosomes to be internalized and deliver functionally active biomolecules such as nucleic acids into recipient cells. Ready-to-use exosomes derived from human embryonic kidney cells (HEK 293) or murine dendritic cells (JAWS) can be transfected with nucleic acids including siRNA, miRNAs, mRNAs as well as plasmid DNA and added to a variety of hard-to-transfect cells for enhanced functional delivery. In addition, this technology can be used to create stable cell lines which is particularly useful for cells that are refractory to transfection using the piggyBac transposon system. EV shuttles also offer a significant advantage over virus-based transduction methodology because they are non-viral, easy to handle and cost effective method to deliver RNA and DNA into target cells.

For more details please refer to the specific products.


Easy exosome shuttle loading protocol

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The EV-Entry reagent significantly enhances the uptake and cargo unloading into recipient cells. See performance data below. The Reagent is sold separately.

The EV-Entry System
Extracellular vesicles (EVs), including exosomes, are nanocarriers used by cells to transport RNA and protein signals and are central to intercellular communication. EVs can be added to recipient cells to deliver exogenous cargo. The EV-Entry reagent system not only enhances the rate of uptake of EVs by the recipient cells but also increases the offloading of the transported cargo into the cytoplasm of the recipient cells. EV-Entry boosts the delivery of both RNA and protein cargo. The EV-Entry system is comprised of Reagent A and B components that are mixed together with EVs or exosomes just prior to use. The protocol takes less than an hour and highly efficient at enabling EVs and exosomes to deliver cargo to recipient cells.




Technical References

Peterson MF, Otoc N, Sethi JK, Gupta A, Antes TJ. Integrated systems for exosome investigation. Methods. 2015 Apr 24. pii: S1046-2023(15)00161-9.

Marcus ME, Leonard JN. FedExosomes: Engineering Therapeutic Biological Nanoparticles that Truly Deliver. Pharmaceuticals (Basel). 2013;6(5):659-80.

Zomer A, Vendrig T, Hopmans ES, van Eijndhoven M, Middeldorp JM, Pegtel DM. Exosomes: Fit to deliver small RNA. Commun Integr Biol. 2010 Sep;3(5):447-50.

Pegtel DM, Cosmopoulos K, Thorley-Lawson DA, van Eijndhoven MA, Hopmans ES, Lindenberg JL, de Gruijl TD, Wurdinger T, Middeldorp JM. Functional delivery of viral miRNAs via exosomes. Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6328-33.

Momen-Heravi F, Bala S, Bukong T, Szabo G. Exosome-mediated delivery of functionally active miRNA-155 inhibitor to macrophages. Nanomedicine. 2014 Mar 29. pii: S1549-9634(14)00132-4.

Related Links

ExoFect Exosome Transfection Reagent

 

 

Description Cat# Size Price    
EV Shuttle Kit Human HEK293 cell exosomes EVS105A-1-SBI 5 reactions 495 € DETAILS   Add to Cart 
EV Shuttle Kit Human HEK293 cell exosomes EVS110A-1-SBI 10 reactions 840 € DETAILS   Add to Cart 
EV Shuttle Kit Mouse JAWS II Mouse bone marrow dendritic cell exosomes EVS205A-1-SBI 5 reactions 495 € DETAILS   Add to Cart 
EV Shuttle Kit Mouse JAWS II Mouse bone marrow dendritic cell exosomes EVS210A-1-SBI 10 reactions 840 € DETAILS   Add to Cart 
EV-Entry System for Exosome Delivery EVEN105A-1-SBI 5 reactions 344 € DETAILS   Add to Cart 
EV-Entry System for Exosome Delivery EVEN110A-1-SBI 10 reactions 553 € DETAILS   Add to Cart 

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